Gpam (NM_008149) Mouse Tagged ORF Clone
CAT#: MG210843
- TrueORF®
Gpam (tGFP-tagged) - Mouse glycerol-3-phosphate acyltransferase, mitochondrial (Gpam)
ORF Plasmid: DDK
"NM_008149" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,120.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | GPAT; GPAT1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210843 representing NM_008149
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGTCTTCAGTGACAGTTGGCACAATAGACGTTTCTTATCTGCCCAGTTCATCGGAATACAGCC TTGGCCGATGTAAACACACCAGTGAGGACTGGGTTGACTGTGGGTTCAAACCTACCTTCTTCAGATCTGC AACACTGAAATGGAAGGAGAGCCTTATGAGCCGGAAGAGGCCCTTCGTGGGAAGGTGCTGCTATTCCTGC ACGCCACAGAGCTGGGAAAGGTTTTTCAACCCCAGTATCCCATCTCTGGGTTTGCGGAATGTTATTTATA TCAATGAAACGCACACAAGGCACAGAGGATGGCTGGCGAGACGGCTGTCTTACATCCTTTTTGTTCAAGA GCGAGACGTCCATAAGGGCATGTTTGCCACCAGTGTTACTGAGAATGTACTGAGCAGCAGCAGAGTCCAA GAGGCAATTGCTGAAGTGGCTGCGGAGTTGAACCCAGATGGATCTGCCCAGCAGCAGTCCAAAGCCATCC AGAAGGTGAAAAGGAAAGCCAGGAAGATCCTCCAGGAGATGGTCGCCACCGTCTCCCCAGGGATGATCAG GCTGACTGGCTGGGTGTTACTAAAGCTCTTCAACAGCTTCTTCTGGAACATTCAGATTCACAAGGGTCAA CTCGAGATGGTCAAGGCTGCAACTGAGACGAACCTGCCGCTCTTGTTTCTGCCGGTGCACAGATCCCACA TTGACTACCTGTTGCTCACCTTCATCCTCTTTTGCCACAACATCAAGGCGCCGTACATCGCCTCGGGCAA TAATCTCAACATCCCCGTCTTCAGTACCTTGATTCACAAGCTTGGGGGCTTTTTCATAAGACGGAGGCTC GATGAAACCCCAGATGGACGCAAAGACATTCTGTACAGAGCGTTGCTCCATGGGCATGTAGTTGAACTCC TCCGACAGCAGCAGTTCCTGGAGATCTTCCTGGAAGGCACCCGCTCCCGCAGTGGCAAGACCTCCTGTGC CCGGGCAGGGCTCCTCTCAGTGGTAGTGGATACTCTGTCGTCCAACACCATCCCCGACATCCTCGTCATA CCCGTGGGCATCTCGTATGATCGCATAATCGAAGGTCACTACAATGGCGAACAGTTGGGAAAGCCCAAGA AGAACGAGAGCCTCTGGAGTGTGGCGAGAGGCGTTATCAGAATGCTGCGGAAAAACTACGGCTACGTCCG AGTGGATTTTGCACAGCCATTTTCCTTGAAGGAATATTTAGAAGGCCAGAGTCAGAAACCTGTATCTGCC CCCCTTTCTCTGGAGCAAGCACTGTTACCAGCGATCCTTCCTTCAAGACCGAATGATGTTGCTGATGAAC ATCAAGACCTATCCAGTAACGAGTCCAGAAACCCAGCAGACGAAGCCTTCCGACGAAGGCTGATTGCAAA CCTGGCTGAGCACATTCTCTTCACCGCCAGCAAGTCCTGCGCTATCATGTCCACCCACATTGTGGCCTGT CTGCTCCTCTACAGACACAGGCAGGGAATCCATCTCTCCACGCTTGTGGAAGACTTCTTTGTGATGAAGG AGGAAGTCCTAGCTCGCGATTTCGACCTAGGCTTCTCCGGGAATTCAGAAGATGTCGTCATGCATGCTAT TCAGCTTCTGGGGAACTGTGTCACAATCACCCACACGAGCAGGAAAGATGAGTTTTTTATTACTCCCAGC ACAACTGTCCCGTCAGTCTTTGAACTCAACTTCTACAGCAATGGCGTACTTCATGTGTTCATCATGGAAG CCATCATAGCTTGCAGCATCTATGCAGTCCTGAATAAGAGGTGCTCTGGAGGGTCCGCTGGAGGCCTCGG CAACCTGATCAGCCAGGAGCAGCTGGTGAGGAAGGCCGCCAGCCTGTGCTACCTTCTCTCTAACGAAGGT ACCATTTCTCTGCCCTGCCAGACTTTTTACCAAGTTTGTCATGAGACAGTTGGCAAGTTCATCCAGTATG GCATTCTCACAGTGGCAGAGCAAGATGACCAGGAAGATGTCAGTCCTGGCCTTGCAGAACAGCAGTGGGA CAAGAAGCTTCCGGAACTGAACTGGAGAAGTGACGAGGAAGATGAAGACAGTGACTTTGGTGAGGAGCAG CGAGATTGCTATCTCAAGGTGAGCCAGTCCAAGGAGCACCAGCAATTCATCACCTTTCTTCAGAGGCTTC TAGGTCCCCTGCTAGAAGCCTACAGCTCTGCTGCCATCTTTGTCCACAACTTCAGCGGTCCAGTTCCCGA GTCTGAGTACCTGCAGAAACTGCACAGGTACCTTATCACCAGGACGGAAAGGAACGTTGCCGTGTACGCT GAGAGTGCCACATACTGTCTCGTGAAGAACGCTGTGAAAATGTTTAAGGACATCGGGGTTTTCAAAGAGA CCAAGCAAAAGCGAGTGTCTGTTCTAGAACTGAGCAGTACTTTCCTACCTCAGTGCAACCGGCAGAAACT CCTAGAGTATATTCTGAGTTTTGTGGTGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210843 representing NM_008149
Red=Cloning site Green=Tags(s) MEESSVTVGTIDVSYLPSSSEYSLGRCKHTSEDWVDCGFKPTFFRSATLKWKESLMSRKRPFVGRCCYSC TPQSWERFFNPSIPSLGLRNVIYINETHTRHRGWLARRLSYILFVQERDVHKGMFATSVTENVLSSSRVQ EAIAEVAAELNPDGSAQQQSKAIQKVKRKARKILQEMVATVSPGMIRLTGWVLLKLFNSFFWNIQIHKGQ LEMVKAATETNLPLLFLPVHRSHIDYLLLTFILFCHNIKAPYIASGNNLNIPVFSTLIHKLGGFFIRRRL DETPDGRKDILYRALLHGHVVELLRQQQFLEIFLEGTRSRSGKTSCARAGLLSVVVDTLSSNTIPDILVI PVGISYDRIIEGHYNGEQLGKPKKNESLWSVARGVIRMLRKNYGYVRVDFAQPFSLKEYLEGQSQKPVSA PLSLEQALLPAILPSRPNDVADEHQDLSSNESRNPADEAFRRRLIANLAEHILFTASKSCAIMSTHIVAC LLLYRHRQGIHLSTLVEDFFVMKEEVLARDFDLGFSGNSEDVVMHAIQLLGNCVTITHTSRKDEFFITPS TTVPSVFELNFYSNGVLHVFIMEAIIACSIYAVLNKRCSGGSAGGLGNLISQEQLVRKAASLCYLLSNEG TISLPCQTFYQVCHETVGKFIQYGILTVAEQDDQEDVSPGLAEQQWDKKLPELNWRSDEEDEDSDFGEEQ RDCYLKVSQSKEHQQFITFLQRLLGPLLEAYSSAAIFVHNFSGPVPESEYLQKLHRYLITRTERNVAVYA ESATYCLVKNAVKMFKDIGVFKETKQKRVSVLELSSTFLPQCNRQKLLEYILSFVVL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_008149 |
ORF Size | 2481 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008149.4 |
RefSeq Size | 3852 bp |
RefSeq ORF | 2484 bp |
Locus ID | 14732 |
UniProt ID | Q61586 |
Gene Summary | Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201591 | Gpam (untagged) - Mouse glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 9,096.00 |
|
MR210843 | Gpam (Myc-DDK-tagged) - Mouse glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), nuclear gene encoding mitochondrial protein |
CNY 9,088.00 |
|
MR210843L3 | Lenti ORF clone of Gpam (Myc-DDK-tagged) - Mouse glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), nuclear gene encoding mitochondrial protein |
CNY 10,170.00 |
|
MR210843L4 | Lenti ORF clone of Gpam (mGFP-tagged) - Mouse glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), nuclear gene encoding mitochondrial protein |
CNY 10,170.00 |