Elmo2 (BC023954) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	CED-12, mKIAA1834
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210342 representing BC023954 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCCTCCGTCTGACATTGTCAAAGTGGCCATCGAGTGGCCAGGTGCTAACGCCCAGCTCCTTGAAA TTGACCAGAAACGGCCGCTGGCATCCATCATCAAGGAGGTGTGCGATGGGTGGTCACTGCCGAACCCGGA GTACTACACCCTCCGCTATGCAGATGGGCCTCAGCTCTACGTCACGGAGCAGACCCGAAATGACATTAAG AACGGGACAATCTTACAACTGGCCGTCTCCCCGTCCCGGGCTGCACGCCAGCTGATGGAAAGGACCCAGT CATCTAGTATGGAGACCCGGCTGGATGCCATGAAGGAGTTGGCTAAGCTCTCAGCTGACGTGACTTTCGC CACGGAGTTCATTAACATGGACGGCATCATTGTGCTGACGAGGCTCGTGGAGAGTGGGACCAAGCTCCTG TCCCACTACAGTGAGATGCTGGCATTCACCCTGACTGCCTTCTTAGAGCTCATGGATCATGGCATTGTCT CCTGGGACATGGTTTCAGTCACCTTTATTAAGCAGATTGCAGGGTACGTGAGCCAGCCCATGGTCGATGT GTCCATCCTCCAGCGGTCCCTGGCCATCCTAGAGAGCATGGTACTAAACAGCCAGAGCCTGTACCAGAAG ATAGCGGAGGAGATCACCGTGGGACAGCTCATCTCCCACCTGCAGGTCTCCAACCAGGAGATCCAGACCT ACGCCATTGCTCTGATTAACGCGCTGTTCCTGAAGGCCCCCGAAGACAAGAGACAGGACAAGCACCTTAA CCCTCTAGACCTGCCCGTCACTGACATGGCCAATGCCTTTGCACAGAAGCACCTTCGGTCCATAATCCTG AACCATGTGATCAGAGGGAATCGTCCAATCAAAACAGAGATGGCCCATCAGCTGTATGTCCTTCAGGTCT TGACCTTTAACCTTCTGGAAGAAAGAATGATGACCAAGATGGATCCCAATGACCAGGCTCAGAGAGACAT TATATTTGAACTGAGGAGGATTGCCTTCGACGCAGAGTCTGACCCCAGCAACGTCCCCGGGAGTGGGACT GAAAAGCGCAAGGCCATGTATACCAAGGACTATAAAATGCTGGGCTTCACCAACCATATCAACCCAGCCT TGGACTTCACCCAGACTCCTCCTGGAATGCTGGCGCTGGACAACATGCTGTACCTGGCTAAAGTCCACCA GGACACCTACATCCGGATCGTGCTGGAGAACAGCAGTCGGGAGGACAAACACGAGTGTCCGTTCGGCCGC AGTGCCATCGAGCTCACCAAGATGCTCTGTGAGATCCTGCAGGTCGGGGAGCTCCCTAATGAAGGGCGCA ATGACTACCACCCCATGTTCTTCACCCACGACCGAGCCTTCGAGGAACTCTTCGGGATCTGCATCCAGCT GCTGAACAAGACCTGGAAGGAGATGAGGGCGACAGCCGAGGATTTCAACAAGGTTATGCAAGTTGTCCGA GAGCAGATCACCCGGGCTCTGCCCTCTAAACCCAACTCTTTGGATCAGTTCAAGAGTAAACTTCGTAGCC TGAGCTACTCAGAAATTCTGCGGTTGCGCCAGTCTGAGAGGATGAGCCAGGATGATTTCCAGTCCCCACC AATTGTGGAGCTTCGAGAGAAGATACAGCCTGAGATCCTGGAGCTGATCAAGCAACAGCGCCTCAACCGG CTATGCGAGGGCAGCAGCTTCCGGAAAATCGGGAACCGTCGTCGGCAAGAGAGGTTCTGGCACTGCCGCT TGGCACTGAACCACAAGGTTTTGCATTACGGTGACTTGGATGACAACCCTCAAGGGGAGGTGACATTCGA ATCCCTGCAGGAGAAAATTCCTGTTGCAGATATTAAGGCCATTGTTACTGGAAAAGACTGTCCTCACATG AAAGAGAAGAGTGCCCTGAAACAGAACAAGGAGGTGTTGGAATTGGCCTTCTCCATCCTGTATGATCCTG ATGAGACACTGAATTTCATTGCTCCCAACAAGTATGAGTACTGCATCTGGATTGATGGACTCAGCGCTCT CCTGGGGAAGGATATGTCCAGCGAGCTAACCAAGAGCGACTTGGACACGCTGCTGAGCATGGAGATGAAG CTGCGGCTTCTGGACTTGGAGAACATTCAGATCCCCGAGGCGCCACCTCCGGTCCCCAAGGAGCCCAGCA GCTATGACTTCGTCTATCACTATGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210342 representing BC023954 Red=Cloning site Green=Tags(s) MPPPSDIVKVAIEWPGANAQLLEIDQKRPLASIIKEVCDGWSLPNPEYYTLRYADGPQLYVTEQTRNDIK NGTILQLAVSPSRAARQLMERTQSSSMETRLDAMKELAKLSADVTFATEFINMDGIIVLTRLVESGTKLL SHYSEMLAFTLTAFLELMDHGIVSWDMVSVTFIKQIAGYVSQPMVDVSILQRSLAILESMVLNSQSLYQK IAEEITVGQLISHLQVSNQEIQTYAIALINALFLKAPEDKRQDKHLNPLDLPVTDMANAFAQKHLRSIIL NHVIRGNRPIKTEMAHQLYVLQVLTFNLLEERMMTKMDPNDQAQRDIIFELRRIAFDAESDPSNVPGSGT EKRKAMYTKDYKMLGFTNHINPALDFTQTPPGMLALDNMLYLAKVHQDTYIRIVLENSSREDKHECPFGR SAIELTKMLCEILQVGELPNEGRNDYHPMFFTHDRAFEELFGICIQLLNKTWKEMRATAEDFNKVMQVVR EQITRALPSKPNSLDQFKSKLRSLSYSEILRLRQSERMSQDDFQSPPIVELREKIQPEILELIKQQRLNR LCEGSSFRKIGNRRRQERFWHCRLALNHKVLHYGDLDDNPQGEVTFESLQEKIPVADIKAIVTGKDCPHM KEKSALKQNKEVLELAFSILYDPDETLNFIAPNKYEYCIWIDGLSALLGKDMSSELTKSDLDTLLSMEMK LRLLDLENIQIPEAPPPVPKEPSSYDFVYHYG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC023954
ORF Size	2198 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC023954, AAH23954
RefSeq Size	3501 bp
RefSeq ORF	2198 bp
Locus ID	140579
Gene Summary	Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).[UniProtKB/Swiss-Prot Function]

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