Elmo2 (BC023954) Mouse Tagged ORF Clone

CAT#: MG210342

  • TrueORF®

Elmo2 (tGFP-tagged) - Mouse engulfment and cell motility 2, ced-12 homolog (C



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Mouse Protein / Lysate Request

CNY 6,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms CED-12, mKIAA1834
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210342 representing BC023954
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCCTCCGTCTGACATTGTCAAAGTGGCCATCGAGTGGCCAGGTGCTAACGCCCAGCTCCTTGAAA
TTGACCAGAAACGGCCGCTGGCATCCATCATCAAGGAGGTGTGCGATGGGTGGTCACTGCCGAACCCGGA
GTACTACACCCTCCGCTATGCAGATGGGCCTCAGCTCTACGTCACGGAGCAGACCCGAAATGACATTAAG
AACGGGACAATCTTACAACTGGCCGTCTCCCCGTCCCGGGCTGCACGCCAGCTGATGGAAAGGACCCAGT
CATCTAGTATGGAGACCCGGCTGGATGCCATGAAGGAGTTGGCTAAGCTCTCAGCTGACGTGACTTTCGC
CACGGAGTTCATTAACATGGACGGCATCATTGTGCTGACGAGGCTCGTGGAGAGTGGGACCAAGCTCCTG
TCCCACTACAGTGAGATGCTGGCATTCACCCTGACTGCCTTCTTAGAGCTCATGGATCATGGCATTGTCT
CCTGGGACATGGTTTCAGTCACCTTTATTAAGCAGATTGCAGGGTACGTGAGCCAGCCCATGGTCGATGT
GTCCATCCTCCAGCGGTCCCTGGCCATCCTAGAGAGCATGGTACTAAACAGCCAGAGCCTGTACCAGAAG
ATAGCGGAGGAGATCACCGTGGGACAGCTCATCTCCCACCTGCAGGTCTCCAACCAGGAGATCCAGACCT
ACGCCATTGCTCTGATTAACGCGCTGTTCCTGAAGGCCCCCGAAGACAAGAGACAGGACAAGCACCTTAA
CCCTCTAGACCTGCCCGTCACTGACATGGCCAATGCCTTTGCACAGAAGCACCTTCGGTCCATAATCCTG
AACCATGTGATCAGAGGGAATCGTCCAATCAAAACAGAGATGGCCCATCAGCTGTATGTCCTTCAGGTCT
TGACCTTTAACCTTCTGGAAGAAAGAATGATGACCAAGATGGATCCCAATGACCAGGCTCAGAGAGACAT
TATATTTGAACTGAGGAGGATTGCCTTCGACGCAGAGTCTGACCCCAGCAACGTCCCCGGGAGTGGGACT
GAAAAGCGCAAGGCCATGTATACCAAGGACTATAAAATGCTGGGCTTCACCAACCATATCAACCCAGCCT
TGGACTTCACCCAGACTCCTCCTGGAATGCTGGCGCTGGACAACATGCTGTACCTGGCTAAAGTCCACCA
GGACACCTACATCCGGATCGTGCTGGAGAACAGCAGTCGGGAGGACAAACACGAGTGTCCGTTCGGCCGC
AGTGCCATCGAGCTCACCAAGATGCTCTGTGAGATCCTGCAGGTCGGGGAGCTCCCTAATGAAGGGCGCA
ATGACTACCACCCCATGTTCTTCACCCACGACCGAGCCTTCGAGGAACTCTTCGGGATCTGCATCCAGCT
GCTGAACAAGACCTGGAAGGAGATGAGGGCGACAGCCGAGGATTTCAACAAGGTTATGCAAGTTGTCCGA
GAGCAGATCACCCGGGCTCTGCCCTCTAAACCCAACTCTTTGGATCAGTTCAAGAGTAAACTTCGTAGCC
TGAGCTACTCAGAAATTCTGCGGTTGCGCCAGTCTGAGAGGATGAGCCAGGATGATTTCCAGTCCCCACC
AATTGTGGAGCTTCGAGAGAAGATACAGCCTGAGATCCTGGAGCTGATCAAGCAACAGCGCCTCAACCGG
CTATGCGAGGGCAGCAGCTTCCGGAAAATCGGGAACCGTCGTCGGCAAGAGAGGTTCTGGCACTGCCGCT
TGGCACTGAACCACAAGGTTTTGCATTACGGTGACTTGGATGACAACCCTCAAGGGGAGGTGACATTCGA
ATCCCTGCAGGAGAAAATTCCTGTTGCAGATATTAAGGCCATTGTTACTGGAAAAGACTGTCCTCACATG
AAAGAGAAGAGTGCCCTGAAACAGAACAAGGAGGTGTTGGAATTGGCCTTCTCCATCCTGTATGATCCTG
ATGAGACACTGAATTTCATTGCTCCCAACAAGTATGAGTACTGCATCTGGATTGATGGACTCAGCGCTCT
CCTGGGGAAGGATATGTCCAGCGAGCTAACCAAGAGCGACTTGGACACGCTGCTGAGCATGGAGATGAAG
CTGCGGCTTCTGGACTTGGAGAACATTCAGATCCCCGAGGCGCCACCTCCGGTCCCCAAGGAGCCCAGCA
GCTATGACTTCGTCTATCACTATGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210342 representing BC023954
Red=Cloning site Green=Tags(s)

MPPPSDIVKVAIEWPGANAQLLEIDQKRPLASIIKEVCDGWSLPNPEYYTLRYADGPQLYVTEQTRNDIK
NGTILQLAVSPSRAARQLMERTQSSSMETRLDAMKELAKLSADVTFATEFINMDGIIVLTRLVESGTKLL
SHYSEMLAFTLTAFLELMDHGIVSWDMVSVTFIKQIAGYVSQPMVDVSILQRSLAILESMVLNSQSLYQK
IAEEITVGQLISHLQVSNQEIQTYAIALINALFLKAPEDKRQDKHLNPLDLPVTDMANAFAQKHLRSIIL
NHVIRGNRPIKTEMAHQLYVLQVLTFNLLEERMMTKMDPNDQAQRDIIFELRRIAFDAESDPSNVPGSGT
EKRKAMYTKDYKMLGFTNHINPALDFTQTPPGMLALDNMLYLAKVHQDTYIRIVLENSSREDKHECPFGR
SAIELTKMLCEILQVGELPNEGRNDYHPMFFTHDRAFEELFGICIQLLNKTWKEMRATAEDFNKVMQVVR
EQITRALPSKPNSLDQFKSKLRSLSYSEILRLRQSERMSQDDFQSPPIVELREKIQPEILELIKQQRLNR
LCEGSSFRKIGNRRRQERFWHCRLALNHKVLHYGDLDDNPQGEVTFESLQEKIPVADIKAIVTGKDCPHM
KEKSALKQNKEVLELAFSILYDPDETLNFIAPNKYEYCIWIDGLSALLGKDMSSELTKSDLDTLLSMEMK
LRLLDLENIQIPEAPPPVPKEPSSYDFVYHYG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC023954
ORF Size 2198 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC023954, AAH23954
RefSeq Size 3501 bp
RefSeq ORF 2198 bp
Locus ID 140579
Gene Summary Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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