Dtx3l (BC085093) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AU042200; BC023741
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210070 representing BC085093 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCAGTCCCGACCCGCCGTCCCCGCTACTCGTACGGCTGCGGGAGTCCATCCCCAAGGCGCACA GGAAGCTCGAGATATACTTCCAGAGCCGGGCCTCGGGAGGTGGGGAGTGCTCTGTCCAGCCCGTGGGCCC CAGCGCTCCGGACACCTACGAGGTGAAGTTCCTAAAAAAAGCAGATAAGGAGAAAGTGTTGAAAAAGAGC GAACACGAGATGTTGGTCCATAACAAACCTGTGACCATTGTCCTGGAAACTACTAAAAAGCCAGTAGAGG ACCTGAGACCCAGACTCCCATCCTTGACACAGCCAGTGGAGACACCAAGCTCCAGACCTCCGTCCTTGAC GGGGTCTCTGGATGAAGCACTTTGTGATGACATACATCCCCAGGACGGGCTCGTTTCTAACTCTGTTGAC TCAGTTGTCCAAAAGATCTTTCTTGCTGTGACCGCTGAGCTGAACTGTGACCTGCTCTCTAAAGAGCAGA GAGCATCTATAACCACTGTCTGCCCTCACATCATCAAAAGCATGGAGGGTAGTGATGGAATTAAGAAGGT GTGTGGCAACTTCAAAGATATTGAAAAGATACATCACTTCTTGAGTGAGCAGCTTTTGGAACGGGAGCAG AAACGGAAGGGAAGCGAGCAGAAACGGAAGTGCGCCCCACAGAAACACACACCTCCCGATGTGGAGCGGG AGCCCCCTGATCAGAGCAGTATTCAAGTTCCTGTGCTTCTCCTTGAATATTTCAAGCATGTTAATCCGGG TAGACTAGAGTTCATAGAGTACAAATTTGGTGTAAACATTGAAATCCAAGCTAGTTCTCCCAATATGGTC ACTGTAGGCTTCACCTCCAGCCCATTTGGCAACGTAGAAGAAGCAAGTCAGTCCTTTGTCAGAGACTTTC AGAAATGCTCGCAGTCTCTGAAGCAAGATTGTATCTCTTTAGAGGAGCACCAGAGAGCAAAGGAGGTCAG ACAGGAGCTGAGTCGCTGCTTCCCAAAGCTCTTGATAAAGGGACAGGGAAGAACGCTGACTCTCCTCGGC TCTCCTGCTGACATTTCAGCCGCCACAGAAAAAGTCTCCCAAGGTCTTGGCCTGAGACCTGTGAAAATAA CCGCATCTGGGTACACGACGGGCATCGAGGTCGATTCAACACGCTTTAAGCTTCTAGAGCCTGAACTGCT CCAGGAAATCTCAGAGATCGAGCAGAAGTTTAACACCCGTGGCAAAGTCCAGGAAAAAGGCCAGAAAACC TGCATTCTTTTTGTCCCCAAGGATAAAGACTTAGACCTGTCAGTGCAGTCCTACACAGGTTTTACTGATG CCTTCCAGCGTGCCACGTGGCAGCTGAGGACAGAAGTTCTGTCGCTGAAAGGGTTGGGCAAGGAGAGAGC TCGCTTACACAATACCAAGTTTGCCGACAACTTTAAAAAAGAGCACCCGAATGTGCACTTTGTGACATCT CAGGAGTCAGTGACCTTGACTGGCTTGCCACATCACCTTGCGCAGGCAATGCAGTATGTCTCCAAAAGAA TGGGACTGGCACCGTCATCTGGAGAGAAACTCGCTATGGATCAGGAAACCCCCATGGAGATCAGCAGTAG TGACCCCCATGGAGGTCAGCAGGAGAATGCAGCCTTACCTGCTCCCCGAGGCACCTCTAGCAGCCCTGCA GCTTCGAAGGGGACTGAGGACTACTGTGTCATCTGCATGGATACCATCAGCAACAAGCACGTGCTCCCCA AGTGCAAGCATGAATTCTGCACCTCGTGTATCAGCAAAGCCATGCTTATCAAGCCTGTCTGTCCTGTGTG TCTGACTTCCTACGGCATCCAGAAAGGGAACCAGCCAGAGGGAACCATGTCTTACTCCACTCAAAAAGGG TCACTTCCAGGTTATGAAGGCTGTGGCACCATTGTGATTAATTATGAAATAAAAGATGGCATCCAAACAA AAGAGCACCCAAACCCAGGAAAGGCTTATCATGGAACACGGCGAACTGCATACTTGCCTGATAATACTGA GGGAAGAAAGGTTTTGGATCTGCTCCACGAAGCCTTTAAGCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210070 representing BC085093 Red=Cloning site Green=Tags(s) MASSPDPPSPLLVRLRESIPKAHRKLEIYFQSRASGGGECSVQPVGPSAPDTYEVKFLKKADKEKVLKKS EHEMLVHNKPVTIVLETTKKPVEDLRPRLPSLTQPVETPSSRPPSLTGSLDEALCDDIHPQDGLVSNSVD SVVQKIFLAVTAELNCDLLSKEQRASITTVCPHIIKSMEGSDGIKKVCGNFKDIEKIHHFLSEQLLEREQ KRKGSEQKRKCAPQKHTPPDVEREPPDQSSIQVPVLLLEYFKHVNPGRLEFIEYKFGVNIEIQASSPNMV TVGFTSSPFGNVEEASQSFVRDFQKCSQSLKQDCISLEEHQRAKEVRQELSRCFPKLLIKGQGRTLTLLG SPADISAATEKVSQGLGLRPVKITASGYTTGIEVDSTRFKLLEPELLQEISEIEQKFNTRGKVQEKGQKT CILFVPKDKDLDLSVQSYTGFTDAFQRATWQLRTEVLSLKGLGKERARLHNTKFADNFKKEHPNVHFVTS QESVTLTGLPHHLAQAMQYVSKRMGLAPSSGEKLAMDQETPMEISSSDPHGGQQENAALPAPRGTSSSPA ASKGTEDYCVICMDTISNKHVLPKCKHEFCTSCISKAMLIKPVCPVCLTSYGIQKGNQPEGTMSYSTQKG SLPGYEGCGTIVINYEIKDGIQTKEHPNPGKAYHGTRRTAYLPDNTEGRKVLDLLHEAFKH TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC085093
ORF Size	2075 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC085093, AAH85093
RefSeq Size	5137 bp
RefSeq ORF	2075 bp
Locus ID	209200
Gene Summary	E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses. Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4. In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1). The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. By monoubiquitinating histone H2B HIST1H2BH/H2BJ and thereby promoting chromatin remodeling, positively regulates STAT1-dependent interferon-stimulated gene transcription and thus STAT1-mediated control of viral replication. Independently of its catalytic activity, promotes the sorting of chemokine receptor CXCR4 from early endosome to lysosome following CXCL12 stimulation by reducing E3 ligase ITCH activity and thus ITCH-mediated ubiquitination of endosomal sorting complex required for transport ESCRT-0 components HGS and STAM. In addition, required for the recruitment of HGS and STAM to early endosomes.[UniProtKB/Swiss-Prot Function]

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