Capn9 (BC058748) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2200003B16Rik; GC36; nCL-4; nCL4
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210066 representing BC058748 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTACCTGCATCGGTCCCTGAGGCCACAGCCACAGCCAGTCCCCGGGGATGCCAGGACTATCCACT CCTCAGGTCAGAGCTTTGAGCAGCTGAGGCAGGGCTGCCTGCAGTCTGGCACCTTGTTTGAGGATGCTGA CTTCCCTGCCAGCAATGTCTCCCTGTTCTACAGCGAGAGGCCCCAGGTCCCCTTTGTGTGGAAACGGCCA GGGGAAATTGTGGAAAAACCAGAATTCATTCTTGGAGGGGCCACCAGGACCGACATTTGCCAAGGGGAGC TGGGAGACTGCTGGCTCCTGGCAGCCATTGCCTCCCTAACCCTCAATCAGAAAGCACTGGCCAGGGTGGT TCCCCAGGACCAAGGATTTGGTTCTGGCTATGCCGGGATATTTCATTTCCAGTTCTGGCAGCACAGTGAG TGGCTGGACGTAGTGATTGATGACCGCCTGCCCACCTTCAAGGACCGCCTGGTCTTCCTCCACTCTGCTG ATCACAATGAGTTCTGGAGCGCACTGCTGGAGAAAGCCTATGCCAAGCTTAACGGGAGTTATGAGGCACT GAAGGGAGGCAGTGCCATTGAAGCCATGGAAGACTTCACTGGGGGTGTGGCTGAGAACTTCCAAATCCGA GAGGCACCAGAGGATTTCTTTGAGATTCTGGAGAAGGCTTTGAAGCGTGGTTCCTTGCTGGGATGCTCCA TAGATACCCTGAATGCCTCAGAATCTGAAGCTCGAACATCTCTTGGTCTCATTAAGGGCCATGCCTACAC CGTGACGGGGCTCGATCAGGTAAACTTCCATGGCCAGAGAATCAAGCTCATCAGAGTCCGTAACCCTTGG GGCCAGGTGGAGTGGAACGGGCCATGGAGTGACAGCTCTCCTGAGTGGCGCTCTGTGGACCTGGAGGAGC AGAAGCGCCTGGGCCACACTGCCCTTGATGACGGGGAGTTCTGGATGGCATTCAAAGACTTCAAGATCCA TTTTGACAAAGTGGAGATCTGCAACCTCACACCTGATGCCCTGGAGGACAGCGCCCTCCACAGGTGGGAG GTGACCATCCACCAAGGAAGCTGGGTCCGTGGCTCCACTGCTGGTGGCTGTCGCAACTTCCTGGACACCT TCTGGACCAACCCTCAGATAAAGCTGTCCCTGACCGAGAGGGACGAGGGCCAGGAGGGCTGCACTTTCCT AGCAGCCTTGATGCAGAAAGACCGCAGGAGGCTCAAGAGGTTTGGCGCCAACATGCTGACCATTGGCTAC GCCATTTACCAGTGCCCAGACAAGGACGGACACCTGAGTAGGGACTTTTTCAGGTACCATGCCTCCCTGG CCCGAAGCAAGACGTTCATCAACTTGAGAGAAGTCTCAGAGCGATTCCAGCTGCCCCCGGGAGACTATAT CCTCATCCCCAGCACCTTTGAGCCGCATCAGGAGGCCGACTTCTGCCTGAGAATCTTCTCTGAGAAGAGA GCTGTGACTCGGGACCTGGACGAGAACATAGACATTGACCTTCCTGAGCTTCCAAAGCCGACTCCACAGG AGGAGGAAACAGAAGAGGAACAGCAGTTCCGGGCCCTGTTCCAGCGAGTTGCTGGCGAGGACATGGAGGT GTCAGCTGAGGAGCTTGAATATGTTCTAAATGCTGTTCTGCAGAAGAAAACAGCCCTCAAGTTCAAGAGG CTGAGCCTGCTGTCCTGCAGAAACATCATCTCTCTGATGGATACCAGTGGCAACGGGAAGCTGGAGTTTG AAGAGTTCCGGGTCTTCTGGGACAAGCTGAAACACTGGATGGACCTGTTTCTCCAGTTCGATGTGGACAA GTCTGGCACCATGTCTTCCTATGAGCTGCGGACGGCACTGAAAGCTGCAGGCTTTCAGCTGGGTGGCCAT CTCCTGCAGCTGATTGTCCTCAGGTATGCAGATGAGGACCTCCAGCTGGATTTCGATGACTACCTCAACT GCCTGGTGCGGCTGGAGAATGCAAGCCGGGTGTTCCAATCTCTCAGTGTGAAGAACAAGGACTTCATCCA TCTCAACATAAACGAGTTCATCAGCCTGACCATGAACATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210066 representing BC058748 Red=Cloning site Green=Tags(s) MPYLHRSLRPQPQPVPGDARTIHSSGQSFEQLRQGCLQSGTLFEDADFPASNVSLFYSERPQVPFVWKRP GEIVEKPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVVPQDQGFGSGYAGIFHFQFWQHSE WLDVVIDDRLPTFKDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAENFQIR EAPEDFFEILEKALKRGSLLGCSIDTLNASESEARTSLGLIKGHAYTVTGLDQVNFHGQRIKLIRVRNPW GQVEWNGPWSDSSPEWRSVDLEEQKRLGHTALDDGEFWMAFKDFKIHFDKVEICNLTPDALEDSALHRWE VTIHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTERDEGQEGCTFLAALMQKDRRRLKRFGANMLTIGY AIYQCPDKDGHLSRDFFRYHASLARSKTFINLREVSERFQLPPGDYILIPSTFEPHQEADFCLRIFSEKR AVTRDLDENIDIDLPELPKPTPQEEETEEEQQFRALFQRVAGEDMEVSAEELEYVLNAVLQKKTALKFKR LSLLSCRNIISLMDTSGNGKLEFEEFRVFWDKLKHWMDLFLQFDVDKSGTMSSYELRTALKAAGFQLGGH LLQLIVLRYADEDLQLDFDDYLNCLVRLENASRVFQSLSVKNKDFIHLNINEFISLTMNI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058748
ORF Size	2072 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058748, AAH58748
RefSeq Size	2325 bp
RefSeq ORF	2072 bp
Locus ID	73647
Gene Summary	Calcium-regulated non-lysosomal thiol-protease.[UniProtKB/Swiss-Prot Function]

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