Nsun2 (BC013625) Mouse Tagged ORF Clone

CAT#: MG208356

  • TrueORF®

Nsun2 (tGFP-tagged) - Mouse NOL1/NOP2/Sun domain family 2 (cDNA clone MGC:18985 IMAGE:4011674)

ORF Plasmid: DDK tGFP



  "BC013625" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,230.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms D13Wsu123e; Misu
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208356 representing BC013625
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGTAAACCATGACGCATCCAGCATACCTAGACTTACAGTAGATGTGGACGGAAGGAAAGAGATTC
TCTTCTATGATCGAATTTTATGTGATGTCCCTTGCAGTGGCGATGGCACAATGAGAAAAAACATTGATGT
CTGGAAGAAATGGACAACCTTAAACAGCTTGCAGCTCCATGGCCTGCAGCTTCGGATTGCAACTCGAGGT
GCTGAGCAGCTGGCGGAAGGTGGCAGGATGGTGTATTCCACGTGTTCCTTGAACCCCGTGGAGGATGAAG
CAGTGATCGCAGCTCTGCTAGAGAAGAGTGAAGGAGCTCTTGAGCTTGCTGATGTGTCTGCTGAGTTGCC
AGGACTGAAGTGGATGCCTGGAGTCTCACAGTGGAAGGTCATGACTAGAGACGGGCAGTGGTTTGCAGAC
TGGCATGAGGTTCCCCAGGGCAGGCATACACAAATCCGACCTACCATGTTCCCACCAACGGACCTGGAGA
AGCTACAGGCAATGCATCTAGAGCGATGCCTTCGAATCCTGCCCCATCATCAGAATACTGGAGGGTTCTT
TGTGGCAGTATTGGTCAAGAAAGCACCAATGCCGTGGAACAAACGTCAGCCCAAGGTCCAGAATAAATCT
GCAGAAGCCAGAGAACCCAGGGTATCCAGCCATGTGGCTGCCACAGAGGGAAATCCCAGTGACCAGTCTG
AGCTGGAAAGTCAGATGATAACTGGAGCTGGTGACTCAGAAACAGCTCACAACACTGAGAACACAGAGAG
CAATGAGAAGAAAGATGGCGTGTGTGGCCCTCCTCCATCAAAGAAAATGAAGTTGTTTGGATTTAAAGAA
GATCCATTTGTATTCATTCCTGAAGATGATCCTTTATTTCCACCTATTGAGAAGTTTTATGCCTTGGATC
CTTCATTCCCGAGGATGAATCTGTTAACCCGAACCACAGAAGGAAAGAAGCGGCAGCTTTATATGGTCTC
CAAGGAGCTGAGGAATGTACTGCTGAACAACAGCGAGAAGATGAAGGTCATTAACACTGGGATAAAAGTC
TGGTGTCGAAATAACAGTGGTGAAGAATTCGATTGTGCATTCCGTTTGGCACAGGAGGGAATATATACAT
TGTATCCATTTATCAATTCAAGAATCATCACTGTATCAATGGAAGACGTGAAGACACTGTTGACCCAGGA
GAACCCATTCTTTAGAAAACTGAGCAGTGAGGCCTACAGTCAAGTCAAGGACCTCGCAAAGGGAAGTGTT
GTGCTGAAGTATGAGCCAGATTCTGCGAATCCAGACACCCTCCAGTGCCCCATCGTGCTGTGTGGGTGGC
GGGGAAAGGCCTCTATTCGAACTTTTGTGCCCAAAAATGAGCGGCTTCATTATCTCAGGATGATGGGTCT
GGAGGTACTGGGAGAAAAGAAGAAAGAGGGAGTCATTCTTACCAATGAGAATGCTGCCAGCCCAGAGCAG
CCTGGAGATGAGGATGCCAAGCAGACAGCACAAGACCCCTGCGTCCCAGACTCCGTCCCTGGCTGTGATG
CAGCTGCAGCTGAGCCATCCCGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208356 representing BC013625
Red=Cloning site Green=Tags(s)

MVVNHDASSIPRLTVDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRG
AEQLAEGGRMVYSTCSLNPVEDEAVIAALLEKSEGALELADVSAELPGLKWMPGVSQWKVMTRDGQWFAD
WHEVPQGRHTQIRPTMFPPTDLEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKAPMPWNKRQPKVQNKS
AEAREPRVSSHVAATEGNPSDQSELESQMITGAGDSETAHNTENTESNEKKDGVCGPPPSKKMKLFGFKE
DPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKV
WCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKTLLTQENPFFRKLSSEAYSQVKDLAKGSV
VLKYEPDSANPDTLQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNENAASPEQ
PGDEDAKQTAQDPCVPDSVPGCDAAAAEPSR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC013625
ORF Size 1565 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC013625, AAH13625
RefSeq Size 2325 bp
RefSeq ORF 1565 bp
Locus ID 28114
Gene Summary RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors (By similarity). May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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