Taf1a (BC061106) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mTAFI48; TAFI48
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207231 representing BC061106 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGTGATTTCGGTGAAGAGTTGACAAAGCTGGCTGTGGCAGAGGATAACCCAGAAACGTCGGTGC TCTCTAAAACGGGAATGCATTTCCCATGGCTTCACAAGCACGTTGAAGCTGTAGTGACTGGAGGGAAGAA GAGGAAGGATTTTGCTCAGACGACAAGTGCTTGTTTAAGCTTTATCCAGGAAGCCCTGCTGAAGCACCAG TGGCAGCAAGCTGCAGAGTATATGCACAGTTACCTGCAGACCCTGGAGGACTCAGACACCGACAAGAGAC AGGCTGCCCCTGAGATCATTTGGAAGCTTGGAAGTGAAATTCTATTTTACCACCCCAAAAGCAATGTGGA GACTTTTAATAGCTTCGCTGACCGGATGAAAAATATTGGTGTCTTGAATTATTTAAAGATCTCCTTACAA CACGCCTTGTATCTTTTACATCATGGAATGCTTGATGATGCGAACAGGAACCTGAGTAAGGCAGAGACGT GGAGGTATGGAGAAAAGTCGTCTTCTCAGGAAGTGTTGATCAACCTTGTTCAGGCTTACAAAGGGCTTCT GCAGTACTACACTTGGACCAGAAAGAAGATGGAGTTGTCAAAGCTTGATGAAGATGATTACGCTTACGCC GCCAAAACCCGAACCATGCTCAGCCAGAGCTGCAAGACGTCTACAAATATCTGTGCACTGGTCAAAACCC CCGGGGTCTGGGATCCTTTTGTGAAGAGTTATGTGGAGATGCTGGAGTTCTATGGTGATCAAGATGGAGC CCGAGAGATGCTCACCAATTATGCATATGATGAGAAGTTCCCGTCGAACCCCAACGCCCACGTCTACTTA TACGAGTTTCTCAAGAGAGAGAAGGCACCGAGGGCGAAGCTCATAAGTGTTCTTAAGATTTTACATGAGA TTGTGCCATCCCATACACTAATGTTAGAATTCCATACATTGCTTAGAAAATCAGACACAGAAGAACACCA GAAACTGGGCTTGTCTGTGTTATTTGAAGTCCTGGACTTCGCTGGATGCAATAAGAACATAACGGCCTGG AAATACTTGGCAAAGTATCTGAAGCAGATATTAGTGGGAAGCCATCACGAGTGGGTTGAAGAAGAGTGGA AGTCTAGGAGGAACTGGTGGCCAGCTTTTCACTTCAGCTTCTTTTGGGCGAAGAGTGACTGGAAGGCAGA CACTGACTTGGCTTGTGAGAAAGCTTTTGTGGCAGGGGTCCTTTTAGGAAAAGGTTGTAAATATTTTCGG TATATTTTAAAACAAGATCATGAAACCTTGAAGAAGAAAATCAAGCGAATGAAGAAGTCTGTGAAAAAAT ACACTATTGTAAATCCAGGTGTTCACACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207231 representing BC061106 Red=Cloning site Green=Tags(s) MMSDFGEELTKLAVAEDNPETSVLSKTGMHFPWLHKHVEAVVTGGKKRKDFAQTTSACLSFIQEALLKHQ WQQAAEYMHSYLQTLEDSDTDKRQAAPEIIWKLGSEILFYHPKSNVETFNSFADRMKNIGVLNYLKISLQ HALYLLHHGMLDDANRNLSKAETWRYGEKSSSQEVLINLVQAYKGLLQYYTWTRKKMELSKLDEDDYAYA AKTRTMLSQSCKTSTNICALVKTPGVWDPFVKSYVEMLEFYGDQDGAREMLTNYAYDEKFPSNPNAHVYL YEFLKREKAPRAKLISVLKILHEIVPSHTLMLEFHTLLRKSDTEEHQKLGLSVLFEVLDFAGCNKNITAW KYLAKYLKQILVGSHHEWVEEEWKSRRNWWPAFHFSFFWAKSDWKADTDLACEKAFVAGVLLGKGCKYFR YILKQDHETLKKKIKRMKKSVKKYTIVNPGVHT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC061106
ORF Size	1361 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC061106, AAH61106
RefSeq Size	2421 bp
RefSeq ORF	1361 bp
Locus ID	21339
Gene Summary	Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits (By similarity).[UniProtKB/Swiss-Prot Function]

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