Ppp2r3c (BC024754) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	G5pr, G4-1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207229 representing BC024754 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGGAAAGACGTGCTTCGCCGGCGGTTAGCGTCGCCCAACACGGATCCAAAGAGGAAAAAAAGCG AACAAGAATTAAAAGATGAAGAAATGGATTTATTTACCAAATACTACTCAGAGTGGAAAGGAGGTAGAAA AAACACAAACGAGTTCTATAAGACCATACCCCGGTTTTATTACAGGTTGCCAGCTGAAGATGAAGTCTTA CTACAGAAATTACGAGAGGAATCTAGAGCTGTCTTTCTACAGAGGAAAAGCAGAGAACTCTTAGATAACG AAGAGTTGCAGAACTTATGGTTTTTGCTGGATAAACACCAGATACCACCTATGATTGGAGAGGAAGCAAT GATCAATTATGAAAATTTTTTGAAGGTTGGTGAAAAGGCTGGACCAAAGTGCAAGCAATTTTTTACTGCA AAAGTCTTTGCCAAACTCCTTCATACAGATTCATATGGACGAATTTCCATCATGCAGTTCTTTAACTACG TCATGCGAAAAGTTTGGCTGCATCAGACAAGAATAGGACTCAGTTTATATGATGTTGCTGGCCAAGGATA CCTTCGGGAATCAGACCTGGAGAACTACATCCTGGAGCTGATCCCGACGCTGCCACAGCTGGACGGGCTG GAGAAGTCCTTCTACTCCTTCTATGTCTGCACTGCAGTCAGGAAGTTCTTCTTCTTCTTGGACCCTCTAA GAACAGGGAAGATCAAAATTCAAGATATTTTGGCATGCAGTTTTCTAGATGATTTACTGGAGCTAAGAGA TGAGGAATTGTCCAAAGAAAGTCAAGAAACAAATTGGTTTTCTGCTCCTTCTGCCCTGAGGGTCTATGGT CAGTATTTGAATCTTGATAAAGATCATAATGGCATGCTAAGTAAAGAGGAGCTCTCCCGTTACGGAACAG CAACCATGACCAATGTCTTCTTAGACCGAGTTTTCCAGGAGTGTCTCACTTACGATGGAGAAATGGACTA TAAGACCTACCTGGACTTTGTTCTTGCCTTAGAAAACAGAAAGGAGCCTGCAGCTCTGCAGTACATTTTC AAACTGCTGGACATTGAGAACAAGGGATACCTGAATGTCTTTTCCCTTAATTATTTCTTTAGGGCCATAC AAGAACTAATGAAAATCCATGGACAGGATCCTGTGTCATTTCAAGATGTCAAGGATGAAATCTTTGACAT GGTCAAACCAAAGGATCCTTTGAAAATCTCACTTCAGGATTTAATCAACAGTAATCAAGGAGACACAGTC ACTACCATTCTAATTGATCTCAATGGCTTCTGGACCTACGAGAACAGAGAAGCCCTTGTTGCAAATGACA ACGAGAACTCTGCAGATCTTGATGACACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207229 representing BC024754 Red=Cloning site Green=Tags(s) MDWKDVLRRRLASPNTDPKRKKSEQELKDEEMDLFTKYYSEWKGGRKNTNEFYKTIPRFYYRLPAEDEVL LQKLREESRAVFLQRKSRELLDNEELQNLWFLLDKHQIPPMIGEEAMINYENFLKVGEKAGPKCKQFFTA KVFAKLLHTDSYGRISIMQFFNYVMRKVWLHQTRIGLSLYDVAGQGYLRESDLENYILELIPTLPQLDGL EKSFYSFYVCTAVRKFFFFLDPLRTGKIKIQDILACSFLDDLLELRDEELSKESQETNWFSAPSALRVYG QYLNLDKDHNGMLSKEELSRYGTATMTNVFLDRVFQECLTYDGEMDYKTYLDFVLALENRKEPAALQYIF KLLDIENKGYLNVFSLNYFFRAIQELMKIHGQDPVSFQDVKDEIFDMVKPKDPLKISLQDLINSNQGDTV TTILIDLNGFWTYENREALVANDNENSADLDDT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC024754
ORF Size	1361 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC024754, AAH24754
RefSeq Size	2418 bp
RefSeq ORF	1361 bp
Locus ID	59032
Gene Summary	May regulate MCM3AP phosphorylation through phosphatase recruitment. May act as a negative regulator of ABCB1 expression and function through the dephosphorylation of ABCB1 by TFPI2/PPP2R3C complex. May play a role in the activation-induced cell death of B-cells.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.