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Rnf34 (NM_030564) Mouse Tagged ORF Clone
CAT＃: MG205854
- TrueORF®
Rnf34 (tGFP-tagged) - Mouse ring finger protein 34 (Rnf34)
ORF Plasmid: DDK
"NM_030564" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW061037; AW536122; BC004042; C88279; RIFF |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205854 representing NM_030564
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCGGGTGCTACTTCTATGTGGGCTTCATGCTGTGGGCTGCTGAATGAAGTCATGGGAACTGGAG CTGTCCGAGGCCAGCAGGCGGGATTTCCGGGAAGCACTGGCCCATTCAGATTTACACCGAGCTCTGACTT CCCCACCTACCCACCAGCAGCTACTGAAGGACCCAATATAGTTTGCAAAGCCTGTGGGCTTTCGTTTTCA GTCTTTAGAAAGAAGCATGTCTGCTGTGACTGCAAGAAGGATTTCTGCTCCCTCTGCTCAGTCTCCCAAG AAAACCTCCGCAGATGTTCTACTTGTCACCTACTACAAGAGACGGCCTTCCAGCGCCCTCAGTTGATGCG ACTAAAAGTGAAGGACCTGCGGCAGTATCTCCTCCTCAGAAACATACCAACCGACACTTGTCGTGAGAAG GAAGACTTGGTGGATCTAGTACTGTGCCACCGTGGACTAGGCTCAGGGGATGACCTGGACTCAAGTAGCC TGAATTCCTCAAGGTCCCAGACTTCTAGTTTTTTTACACAGTCCCTCTTTTCAAATTACACTCCTCCCTC TGCTACTGTGTCCTCCTTCCAGGGAGAGCTCATGGACAGAGATGGCGCCTTCAGATCAGAAGTCCTGGCA CAGGTACAAAGTGAAATAGCTTCAGCAAACACAGATGATGATGACGACGACGATGATGATGACGACGATG ATGAAGATGACGACGACGAACAAGAGGAGGAGGAACAGAACCCGGGGCTCTCTAAAAAGAAAGCAAGAGC TTCCCTGTCTGACCTCTCAAGCCTTGAAGAGGTAGAAGGGATGAGTGTGCGCCAGCTGAAGGAGATCCTG GCTCGGAATTTTGTCAACTATTCTGGCTGTTGTGAAAAATGGGAGCTGGTAGAGAAAGTCAACCGGCTGT ACAAAGAGAATGAAGAAAACCAAAAGTCATATGGTGAGCGGATGCAACTTCAGGATGAGGAAGATGACAG CCTGTGCCGAATCTGCATGGATGCGGTCATTGACTGTGTCCTGCTGGAGTGCGGGCACATGGTCACCTGC ACCAAGTGTGGCAAGCGAATGAGTGAGTGTCCCATCTGCAGGCAGTATGTGGTTCGAGCTGTGCATGTTT TTAAGTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205854 representing NM_030564
Red=Cloning site Green=Tags(s) MKAGATSMWASCCGLLNEVMGTGAVRGQQAGFPGSTGPFRFTPSSDFPTYPPAATEGPNIVCKACGLSFS VFRKKHVCCDCKKDFCSLCSVSQENLRRCSTCHLLQETAFQRPQLMRLKVKDLRQYLLLRNIPTDTCREK EDLVDLVLCHRGLGSGDDLDSSSLNSSRSQTSSFFTQSLFSNYTPPSATVSSFQGELMDRDGAFRSEVLA QVQSEIASANTDDDDDDDDDDDDDEDDDDEQEEEEQNPGLSKKKARASLSDLSSLEEVEGMSVRQLKEIL ARNFVNYSGCCEKWELVEKVNRLYKENEENQKSYGERMQLQDEEDDSLCRICMDAVIDCVLLECGHMVTC TKCGKRMSECPICRQYVVRAVHVFKS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030564 |
| ORF Size | 1128 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030564.1, NP_085041.1 |
| RefSeq Size | 1980 bp |
| RefSeq ORF | 1131 bp |
| Locus ID | 80751 |
| UniProt ID | Q99KR6 |
| Gene Summary | E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200181 | Rnf34 (untagged) - Mouse ring finger protein 34 (Rnf34), (10ug) |
CNY 3,656.00 |
|
| MR205854 | Rnf34 (Myc-DDK-tagged) - Mouse ring finger protein 34 (Rnf34) |
CNY 3,656.00 |
|
| MR205854L3 | Lenti ORF clone of Rnf34 (Myc-DDK-tagged) - Mouse ring finger protein 34 (Rnf34) |
CNY 4,750.00 |
|
| MR205854L4 | Lenti ORF clone of Rnf34 (mGFP-tagged) - Mouse ring finger protein 34 (Rnf34) |
CNY 4,750.00 |
