Zdhhc16 (BC010835) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1500015N03Rik; Aph2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205528 representing BC010835 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGCCAGCGAAGTCTGCTGTTGGGGCCAGCCCGCCTCTGCCTGCGCCTCCTTTTGCTCCTGGGCT ACCGGCGTCGATGCCCACCTCTGCTCCGGGGCCTGGTACAGCGCTGGCGTTATGGCAAGGTTTGCCTGCG CTCCCTGCTCTACAATTCCTTTGGAGGCAGTGACACTGCCGTTGATGCTGCCTTTGAGCCTGTCTACTGG CTGGTGGACAATGTGATCCGCTGGTTTGGAGTGGTGTTTGTGGTGCTGGTGATCGTGCTGACCGGGTCCA TCGTGGCCATTGCTTATCTGTGTGTCCTGCCCCTCATCCTCCGAACCTACTCGGTACCACGGCTCTGCTG GCATTTCTTCTATAGTCACTGGAATCTGATCCTCATCGTCTTCCATTACTACCAGGCCATCACCACTCCT CCTGGATACCCACCCCAGGGCAGGAATGACATTGCTACTGTCTCCATCTGTAAGAAGTGCATTTATCCCA AGCCAGCCCGAACACACCACCGCAGCATCTGTAATAGGTGTGTGCTGAAGATGGATCATCACTGCCCCTG GCTAAACAACTGTGTGGGCCACTATAACCATCGCTACTTCTTCTCTTTCTGCTTTTTCATGACTCTGGGC TGTGTCTACTGTAGCTATGGAAGTTGGGACCTTTTCCGGGAGGCTTATGCTGCCATTGAGACGTACCACC AGACTCCACCACCTACCTTCTCCTTTCGAGAAAGGATAACTCACAAGAGTCTTGTCTACCTCTGGTTCCT GTGCAGTTCTGTGGCCCTGGCCCTGGGTGCCCTAACCATGTGGCATGCTGTTCTCATCAGTCGGGGTGAG ACTAGCATCGAAAGGCATATCAACAAGAAGGAGAGGCGCCGGTTACAGGCCAAGGGCAGGGTGTTTAGGA ATCCTTACAACTATGGCTGCTTGGACAACTGGAAGGTGTTCCTAGGTGTGGACACAGGAAGGCATTGGCT GACCCGGGTGCTGTTACCTTCTAGTCACCTGCCCCATGGGAATGGGATGAGCTGGGACCCTCCTCCCTGG GTGACTGCTCACTCAGCCTCTGTGATGGCCGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205528 representing BC010835 Red=Cloning site Green=Tags(s) MRGQRSLLLGPARLCLRLLLLLGYRRRCPPLLRGLVQRWRYGKVCLRSLLYNSFGGSDTAVDAAFEPVYW LVDNVIRWFGVVFVVLVIVLTGSIVAIAYLCVLPLILRTYSVPRLCWHFFYSHWNLILIVFHYYQAITTP PGYPPQGRNDIATVSICKKCIYPKPARTHHRSICNRCVLKMDHHCPWLNNCVGHYNHRYFFSFCFFMTLG CVYCSYGSWDLFREAYAAIETYHQTPPPTFSFRERITHKSLVYLWFLCSSVALALGALTMWHAVLISRGE TSIERHINKKERRRLQAKGRVFRNPYNYGCLDNWKVFLGVDTGRHWLTRVLLPSSHLPHGNGMSWDPPPW VTAHSASVMAV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC010835
ORF Size	1085 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC010835, AAH10835
RefSeq Size	1816 bp
RefSeq ORF	1085 bp
Locus ID	74168
Gene Summary	Palmitoyl acyltransferase that mediates palmitoylation of proteins such as PLN and ZDHHC6 (PubMed:26644582). Required during embryonic heart development and cardiac function, possibly by mediating palmitoylation of PLN, thereby affecting PLN phosphorylation and homooligomerization (PubMed:26644582). Also required for eye development (PubMed:26644582). Palmitoylates ZDHHC6, affecting the quaternary assembly of ZDHHC6, its localization, stability and function (By similarity). May play a role in DNA damage response (PubMed:27159997). May be involved in apoptosis regulation (PubMed:12021275). Involved in the proliferation of neural stem cells by regulating the FGF/ERK pathway (By similarity).[UniProtKB/Swiss-Prot Function]

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