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Rab5a (BC004842) Mouse Tagged ORF Clone
CAT＃: MG202370
- TrueORF®
Rab5a (tGFP-tagged) - Mouse RAB5A, member RAS oncogene family (cDNA clone MGC:6509 IMAGE:2649317)
ORF Plasmid: DDK
"BC004842" in other vectors (4)
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CNY 4,000.00
| Cited in 1 publication. |
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2410015H04Rik; AI663973; AU021172; nnyRab5a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC004842 |
| ORF Size | 645 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC004842.1 |
| RefSeq Size | 2246 bp |
| RefSeq ORF | 647 bp |
| Locus ID | 271457 |
| Gene Summary | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (By similarity). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (PubMed:18425118).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Fscn1 is required for the trafficking of TGF-ß family type I receptors during endoderm formation
,Liu, Z;Ning, G;Xu, R;Cao, Y;Meng, A;Wang, Q;,
Nat Commun
,PubMed ID 27545838
[Rab5a]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207182 | Rab5a (untagged) - Mouse RAB5A, member RAS oncogene family (cDNA clone MGC:6509 IMAGE:2649317), (10ug) |
CNY 3,230.00 |
|
| MR202370 | Rab5a (Myc-DDK-tagged) - Mouse RAB5A, member RAS oncogene family (cDNA clone MGC:6509 IMAGE:2649317) |
CNY 2,400.00 |
|
| MR202370L3 | Lenti ORF clone of Rab5a (Myc-DDK-tagged) - Mouse RAB5A, member RAS oncogene family (cDNA clone MGC:6509 IMAGE:2649317) |
CNY 4,090.00 |
|
| MR202370L4 | Lenti ORF clone of Rab5a (mGFP-tagged) - Mouse RAB5A, member RAS oncogene family (cDNA clone MGC:6509 IMAGE:2649317) |
CNY 4,090.00 |
