Ube2m (NM_145578) Mouse Tagged ORF Clone
CAT#: MG201663
- TrueORF®
Ube2m (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) (Ube2m)
"NM_145578" in other vectors (5)
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CNY 2,850.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2510040H03Rik; Ubc-rs2; UBC12 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201663 representing NM_145578
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAAGCTGTTCTCGCTGAAGCAGCAGAAGAAGGAGGAGGAGTCGGCCGGCGGCACCAAGGGCAGCA GCAAGAAGGCGTCGGCGGCGCAGCTCCGGATTCAGAAGGACATTAACGAGCTGAACCTGCCCAAGACGTG TGACATCAGCTTCTCAGACCCAGACGACCTCCTCAACTTCAAGCTGGTGATCTGTCCTGATGAAGGCTTC TACAAAAGTGGCAAGTTTGTATTCAGCTTTAAGGTGGGACAGGGTTACCCACATGACCCTCCCAAGGTGA AGTGTGAAACAATGGTTTATCATCCCAACATTGACCTCGAGGGCAACGTCTGCCTCAACATCCTCAGAGA GGACTGGAAGCCAGTCCTTACGATAAACTCCATAATTTATGGCCTGCAGTATCTCTTCTTGGAGCCGAAC CCTGAGGACCCACTGAACAAGGAGGCTGCTGAGGTCCTGCAGAACAACCGGCGGCTGTTTGAACAAAATG TGCAGCGCTCCATGAGAGGTGGTTACATCGGGTCCACCTACTTCGAGCGCTGCCTGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201663 representing NM_145578
Red=Cloning site Green=Tags(s) MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGF YKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPN PEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_145578 |
ORF Size | 1292 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145578.3, NP_663553.1 |
RefSeq Size | 1199 bp |
RefSeq ORF | 552 bp |
Locus ID | 22192 |
UniProt ID | P61082 |
Gene Summary | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202916 | Ube2m (untagged) - Mouse ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) (Ube2m), transcript variant 1, (10ug) |
CNY 2,400.00 |
|
MG221562 | Ube2m (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2M (UBC12 homolog yeast) (Ube2m) transcript variant 1, (10ug) |
CNY 2,090.00 |
|
MR221562 | Ube2m (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) (Ube2m), transcript variant 1 |
CNY 1,900.00 |
|
MR221562L3 | Lenti ORF clone of Ube2m (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) (Ube2m), transcript variant 1 |
CNY 3,800.00 |
|
MR221562L4 | Lenti ORF clone of Ube2m (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) (Ube2m), transcript variant 1 |
CNY 3,800.00 |