Vcan (NM_001081249) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5430420N07Rik; 9430051N09; Cspg2; DPEAAE; hdf; NG2; PG-M; PG-M(V0); PG-M(V1)
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229771 representing NM_001081249 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGATAAATATGAAAGGCATCTTATGGATGTGCTCAACCTTATTACTAACCCATGCACTACATCAAG CCAAAATGGAAACCAGCCCACCTGTTAAAGGCTCTCTGTCTGGAAAAGTGGTCCTACCTTGTCATTTTTC AACCTTACCTACCTTACCACCCAATTACAACACGAGTGAATTTCTCAGAATCAAATGGTCTAAGATGGAA GTGGACAAAAATGGAAAAGATATAAAGGAGACGACTGTCTTGGTGGCCCAGAACGGAAATATCAAGATTG GTCAGGACTACAAGGGGCGAGTGTCCGTGCCTACACATCCCGATGATGTAGGTGATGCTTCCCTCACCAT GGTCAAACTCCGGGCTAGTGATGCAGGCGTCTACCGATGTGATGTCATGTATGGGATTGAAGACACTCAG GACACCATGTCACTGGCTGTGGATGGTGTTGTGTTTCACTACAGGGCAGCCACCAGCAGGTACACTCTGA ACTTTGCCGCTGCTCAACAGGCTTGTTTGGATATCGGGGCGGTCATAGCAAGCCCAGAGCAGCTGTTTGC CGCCTATGAGGATGGATTTGAGCAGTGTGATGCAGGATGGCTGTCTGATCAAACTGTCAGATATCCCATA CGGGCTCCCCGAGAGGGCTGTTACGGAGACATGATGGGGAAGGAAGGGGTTCGGACCTATGGATTCCGCT CTCCCCAGGAAACCTATGATGTGTATTGTTATGTGGATCATCTGGATGGCGATGTGTTCCACATCACTGC TCCCAGTAAGTTCACCTTCGAGGAGGCCGAAGCAGAGTGTACAAGCAGGGATGCGAGGCTGGCGACTGTT GGAGAACTTCAGGCAGCTTGGAGAAATGGCTTTGACCAATGCGATTACGGCTGGCTGTCGGATGCCAGCG TGCGGCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGAGGTCTACTTGGGGTGAGAACCCTGTATCG TTTTGAGAACCAGACATGCTTCCCTCTCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACCTAAACAG AATATATCAGAGGCAACCACCATCGAAATGAATATCCTCGCAGAAACTTCATCACCCAGTTTATCCAAAG AACCACACATGGTACCTGACAGAGCTACACCAGTCATTCCATTAGCCACCGAATTACCTATTTTTACCAC ACACTTCCCTCCTGCAGGAAATATAGTCAATTCTGAACAGAAATCTGTAGTCTATTCTCAGGCTATCACA GGCAGATTAGCCATGGAATCACCCACAACTACTCGGAATACCATCAACTCTTGGGATCTGAATGACTCCT TGGCTTCTGGGTCAGGGCCACTTGGAATGCCAGACATATCTGAAATTAAGGAGGAGGAACTCCGAAGTAC AACTGTCATCTCCCAACATGCTACTGGCTCACAGGCTGTAATTACAGAGGATACGCAAACACATGAATCA GTTTCACAGATTGAACAAATAGAAGTGGGCCCCTTAGTAACATCCATGGAAATCACAAATCATATTTCTC TCAAGGAATTGCCAGAAAAAAACAAAACACCATATGAATCCACAGAAGTGACCCTGGAACACACGACTGA AATGCCAACGGTAAGCGCCAGTCCTGAATTGGCAACCACAAGTCACTATGGATTCACCTTGAGAGAAGAT GATAGAGAAGACAGAACACTGACAGTTAGATCTGATCAGAGCACTCGGGTCTTTAGCCAAATCCCCGAAG TCATCACAGTATCAAAAACATCAGAAGACACTACCTACTCACAGCTAGGGGACTTAGAGTCCATTTCAAC ATCAACAATAACCATGCTTGGCACAGATAGATCACTGATAGATAAAGAGAAAGAACCCAAAACTAATGGT AAGGTGACTGAAGATGAGTTTGGTCAATCACAGCCTACAACTACCTTCCCATCACAGCATCTTACAGAAG TAGAATTACTTCCATATTCTGGTGACACAACATCAGTAGAGGGAATATCCACAGTTATTTATCCATCATT ACAAACAGATGTGACACAAGGAAGAGAGAGGACAGAAACACCAAGACCAGAGTTAAAAAAAGATCCGTAC ACAGTTGATGAAATACCAGAAAAGGTCACTAAAGATCCATTTATAGGTAAAACAGAAGAAGTCTTCTCTG GAATGCCTCTCTCTACATCTTCCTCAGAGTCCTCAGTGGAAAGGACAGAATCTGTTTCCCCTGCATTGAC AATTGAGAAGTTAACTGTGAAGCCAACAGAAGCAAGAGATGTGGAAGAAATGACAACCCTGACTAGATTA GAAACAGATGTTACAAAGTCTGACAAAGATGTTACAAGAGTTCATTTGACCCACAGTACTTTAAATGTGG AAGTGGTCACAGTATCAAAATGGCCAGGGGATGAAGATAATTCAACTTCAAAGCCTTTACCCTCAACAGA ACATGCAGGCTTTACAAAATTGCCTCCTGTCCCACTTAGTACTATAGGAATAAATGGAAAAGATAAAGAA ATCCCAAGTTTCACAGATGGAGGAGGTGAATACACTCTTTTCCCAGATGGTACTCCAAAACCATTAGAAA AGGTTTCTGAAGAAGATTTAGCATCTGGAGAATTGACAGTCACCTTTCATACATCTACATCAATTGGCAG TGCAGAAAAGTCAGCTTCAGGAGAGCCTACAACTGGAGACAGGTTTCTGCCCACCACAAGCACAGAAGAC CAGGTCATCAATGCAACTGCGGAAGGGAGTGCTTTGGGTGAAGATATAGAAGCCTCCAAGCCACTATTTA CTGGCCCCCCATTTGTCCACACCTCCGATGTAGAAGAATTGGCATTTGTTAATTATAGTAGCACGCAGGA GCCCACTACTTATGTAGACATATCCCATACTAGTCCTCTTTCTATAATTCCCAAGACAGAATGGAGTGTG TTGGAAACTTCTGTTTCACTAGAAGATGAGATTCTAGGCAAATCTGATCAAGATATCCTTGAACAGACTC ATCTAGAAGCAACCATGTCTCCTGGAGCTTTGAGGACAACAGGAGTTTCACAGGGAGAAACCCAGGAAGA ACCACAAACTCCAGGGAGCCCATTTCCTACTTTCAGTTCTACTGCAGTTATGGCCAAGGAGACAACAGCA TTTGAAGAGGGGGAGGGGTCAACATATACTCCCTCTGAAGGCAGATTGATGACAGGTTCTGAAAGAGTCC CAGGTTTAGAAACAACTCCAGTTGGCACATCTTATCCACCTGGTGCAATAACAGACCAGGAAGTGGAAAT GGATACAATGGTAACACTGATGTCAACCATTAGGCCAACAGTGGTTTCAAGTACAGAGCCAGAAGTAATT TATGAAGCAGAAGGTAGTAGTCCAACAGAATTTGCATCAACTTTGAGGCCTTTCCAGACTCATGTCACAC AGCTTATGGAAGAAACTACTGAGGAAGGAAAAAAGGCATCCTTAGATTATACAGATTTAGGCTCAGGGTT ATTTGAACCCAGAGCCACCGAGCTCCCAAAATTTCCAACAACTCCAAGTGATATCAGTGTCTTTACTGCC ATCGACAGCCTTCACAGAACGCCACCCTTGAGTCCGTCTTCATCATTCACTGAGGAACAACGTGTCTTTG AGGAAGAGTCCAGTGAGAAAACAACAGGTGACATACTTCCTGGAGAATCTGTAACACAGCACCCTGTGAC AACTCTCATTGATATCGTAGCCATGAAAACAGAATCTGATATTGATCACATGACCTCAAAACCTCCTGTA ACACAGCCAACAAGACCATCAGTTGTGGAAAGAAAAACAACCTCCAAAACTCAAGAGTTGTCTACTTCAA CACCTGCAGCAGGGACCAAGTTCCACCCTGACATAAATGTTTATATTATTGAGGTCAGAGAAAACAAGAC AGGTCGATTGAGTGATATGATTGTAAGTGGTCATCCAATAGATTCAGAATCTAAAGAAGAGGAACCTTGC AGTGAAGAAACAGATCCACTGCATGATCTGTTTGCTGAAATTTTACCCGAGTTACCAGACTCATTTGAAA TAGACATATATCACAGTGAGGAAGATGAAGACGGAGAGGAGGACTGTGTAAATGCAACGGATGTAACAAC TACTCCGTCAGTGCAGTATATCAATGGGAAGCAGCTCGTTACCACAGTGCCTAAGGACCCGGAAGCTGCA GAAGCTAGGCGTGGCCAGTACGAAAGTGTTGCACCTTCTCAGAATTTCCCAGATAGTTCTGCAACTGACA CCCATCAGTTTATACTAGCAGAAACAGAATCGTCAACTACCATGCAATTTAAGAAATCTAAAGAAGGCAC GGAATTGTTAGAAATCACATGGAAACCCGAGACCTACCCTGAAACACCAGACCATGTTTCAAGTGGTGAG CCTGATGTTTTCCCTACTCTCTCATCCCATGATGGTAAAACCACCAGATGGTCAGAGTCCATCACAGAGA GCAGTCCAAACCTTGAAAATCCAGTGCACAAACAACCTAAGCCTGTCCCTCTGTTTCCTGAAGAGTCTTC AGGAGAGGGTGCCATTGAACAAGCATCCCAGGAAACAATCTTATCTAGGGCTACAGAAGTAGCACTTGGT AAAGAGACAGACCAAAGTCCTACCTTATCTACCTCTAGCATACTTTCAAGTTCTGTGTCAGTAAATGTCT TGGAGGAAGAACCACTTACTCTAACAGGAATCTCACAGACTGATGAATCCATGTCTACTATAGAAAGCTG GGTAGAGATAACACCTAGCCAAACTGTAAAGTTCTCGGAGAGTTCTTCAGCTCCTATCATTGAAGGGTCT GGGGAAGTAGAAGAAAATAAAAATAAAATCTTTAACATGGTAACTGATTTACCACAGAGAGATCCTACAG ATACACTCAGCCCTTTGGATATGAGCAAGATAATGATCACAAACCATCACATTTATATTCCTGCAACCAC CGCTCCTTTGGATTCAAAACTACCTTCTCCAGATGCAAGGCCTACACAGTTTGGAATTCAAACAGCCACC TCTGAGTGGGTTTCCGATAAGTCTTTTGAAGGAAGAAAAAGAGAAGAAGATGAAGAGGGAGCTGTGAATG CAGCCCACCAAGGAGAAGTTCGAGCAGCAACAGAGAGGTCAGATCACTTACTTTTAACACCAGAATTAGA AAGTTCAAATGTAGATGCATCTAGTGATTTGGCTACCTGGGAAGGTTTTATTCTAGAGACAACACCCACA GAGTCTGAAAAGGAAATGGCAAATTCAACTCCTGTTTTTAGAGAAACAATTGGTGTAGCCAATGTGGAGG CACAGCCCTTTGAGCATAGCAGTAGTAGTCATCCCAGGGTTCAGGAAGAGCTGACCACTCTCTCAGGAAA TCCCCCCTCTCTTTTTACGGACCTGGGCTCAGGAGATGCTAGTACTGGTATGGAACTCATCACTGCTTCT TTATTTACATTAGATTTAGAGTCTGAAACCAAAGTCAAAAAGGAATTACCCAGTACTCCGTCTCCCAGTG TGGAGATATCATCCTCTTTTGAGCCAACAGGATTAACCCCGAGTACTGTACTAGACATAGAAATTGCTGG AGTTATGAGCCAGACATCGCAGAAGACACTGATTTCTGAAATATCAGGAAAGCCAACTTCCCAGTCAGGA GTAAGGGATTTGTATACAGGCTTTCCAATGGGAGAAGATTTTAGTGGTGACTTCAGTGAATATCCAACAG TGTCTTATCCAACGATGAAAGAGGAAACAGTTGGGATGGGAGGTTCTGACGATGAACGAGTTAGGGATAC ACAGACTTCATCATCTATACCGACTACCTCAGACAACATCTACCCCGTGCCTGACTCAAAAGGACCTGAC AGTACTGTGGCCAGCACCACAGCCTTCCCCTGGGAAGAGGTCATGTCCTCAGCTGAGGGGTCAGGTGAGC AATTAGCCTCAGTCAGGAGCTCTGTTGGTCCAGTGCTTCCACTAGCTGTGGACATTTTTTCTGGAACAGA GTCTCCATATTTTGATGAAGAGTTTGAAGAAGTGGCTGCAGTGACAGAAGCAAATGAAAGACCCACTGTT TTGCCAACAGCAGCAAGTGGAAATACTGTAGATCTTACAGAAAATGGGTACATAGAGGTAAATAGCACGA TGTCACTGGACTTTCCCCAGACAATGGAGCCATCCAAATTGTGGTCTAAACCAGAAGTCAATCTTGATAA ACAAGAGATTGGAAGAGAAACAGTGACAAAGGAAAAAGCTCAGGGACAAAAGACTTTTGAATCGCTCCAC AGTTCTTTTGCACCAGAACAAACCATTTTGGAAACCCAATCACTTATTGAAACTGAATTCCAAACTTCCG ATTACTCTATGTTAACAACACTGAAAACGTACATTACTAATAAGGAAGTGGAGGAGGAAGGTATGTCCAT AGCGCATATGTCTACTCCAGGTCCAGGGATCAAAGACTTGGAATCATATACTACCCATCCCGAAGCTCCT GGAAAGTCACACTCTTTCTCAGCTACTGCTTTAGTGACTGAATCTGGAGCAGCTAGAAGTGTACTCATGG ATTCCTCAACTCAAGAGGAAGAAAGCATAAAACTCTTCCAAAAGGGTGTGAAACTAACAAATAAAGAGTC AAATGCTGATCTCTCATTCTCTGGACTGGGATCAGGAGAGGCTTTGCCTCCTCTACCTACAACATCAGTG AATTTGACTGACATGAAGCAGATCATTAGCACATTGTATGCTGAGACATCTCACATGGAAAGCTTAGGAA CAAGCATCTTAGGTGATAAAATGGAAGACCATGAAAGAATGGAAGATGTGTCCAATGAAGTTAGAATGCT CATTTCCAAAACAGGCAGCATCTCTCAAGACAGCACAGAGGCACTCGACACAACTTTGAGTCATACCGGA ACAGAAGAACCTACAACTTCAACTCTTCCTTTCGTGAAGCTCATGGACTTGGAACGTTCTCCAAAGCAGA CCCTCAGGTGGGAAGAGGAAACCCAGACCCATAGACCACAAACTATGAGTGGACTGATCTCTAATGAAAA TTCTTCAGCATCAGAAGCTGAAGAAGCAGCAACTTCCCCCACTGCTTTTCTGCCTCAAACTTACAGTGTT GAAATGACCAAACACTTTGCTCCATCAGAGTCACAACCATCCGACTTGTTTAATGTAAACTCTGGAGAGG GATCTGGAGAAGTGGATACTCTTGACTTAGTGTACACTTCTGGAACTACTCAGGCAAGCAGTCAAGGAGA CAGCATGCTTGCTTCTCATGGATTTCTGGAAAAACATCCAGAGGTGTCAAAGACTGAGGCTGGAGCTACT GATGTATCTCCAACAGCCTCAGCAATGTTCCTGCATCACTCAGAGTACAAGAGTTCTCTGTATCCAACCA GCACACTGCCAAGCACAGAGCCATATAAAAGTCCCTCAGAAGGTATTGAAGATGGTTTGCAAGACAATAT CCAATTTGAAGGTTCTACCTTGAAACCTAGCAGAAGAAAAACCACTGAAAGCATTATTATAGATCTAGAC AAAGAGGATAGTAAGGACCTAGGATTGACAATTACTGAGAGTGCTATTGTTGAAATTCTCCCTGAGCTGA CATCAGATAAAAATATTATTATAGACATTGATCACACTAAACCTGTATATGAGTACATCCCTGGAATACA AACAGACCTAGATCCAGAGATAAAATTGGAATCACATGGCAGTAGTGAAGAAAGCCTTCAAGTTCAAGAG AAGTATGAGGGAGCTGTTACCCTTTCTCCAACTGAGGAATCATTTGAAGGCTCTGGTGATGCGCTTCTTG CTGGCTATACTCAAGCAATATACAATGAGTCTGTGACTCCCAACGATGGAAAGCAGGCAGAGGACATAAG CTTTAGTTTTGCCACTGGGATCCCAGTTTCTAGCACAGAGACAGAATTACACACTTTCTTCCCCACAGCG TCAACCCTGCACATTCCTAGTAAACTAACCACAGCTAGTCCGGAGATTGATAAACCCAACATTGAAGCAA TATCTCTGGATGATATATTTGAATCAAGCACCTTGTCTGATGGCCAAGCTATTGCAGACCAGAGTGAAGT AATATCAACATTAGGGCATTTGGAAAAAACACAGGAAGAATATGAAGAAAAGAAATATGGAGGTCCTTCA TTTCAGCCAGAATTCTTTTCAGGAGTGGGGGAAGTATTGACAGATCCCCCTGCTTATGTAAGTATTGGTA GTACATACCTTATAGCTCAGACTTTAACAGAATTACCCAATGTGGTAAGACCATCTGACTCCACACATTA CACTGAAGCAACACCTGAAGTTTCCTCTCTGGCAGAGCTCTCTCCACAGATACCATCTTCTCCCTTCCCT GTCTACGTAGACAATGGAGTCTCTAAATTCCCAGAGGTTCCCCATACAAGTGCTCAGCCAGTTTCTACTG TCACCTCATCACAAAAGTCTATAGAGAGTCCTTTTAAGGAAGTCCATGCTAATATTGAAGAAACCATCAA GCCATTGGGTGGAAATGTCCACAGAACAGAGCCTCCATCCATGTCTCGTGACCCTGCTTTAGATGTTTCA GAAGATGAAAGTAAGCATAAGTTACTAGAAGAACTGGAAACGTCTCCCACCAAACCTGAGACTTCCCAAG ACTTCCCAAACAAAGCAAAGGATCACATTCCAGGAGAAACAGTTGGGATGCTTGCTGGTATCAGAACAAC AGAGAGCGAACCTGTCATTACTGCAGACGACATGGAGCTAGGAGGTGCCACACAGCAGCCACATTCTGCT TCTGCAGCTTTCAGAGTTGAGACAGGTATGGTACCTCAGCCCATCCAACAGGAGCCTGAGAGGCCAACCT TTCCTTCCTTGGAAATTAACCATGAAACACACACATCATTATTTGGAGAATCTATACTGGCAACATCTGA AAAACAAGTGTCTCAAAAAATTCTTGATAATAGTAACCAGGCAACAGTCAGTAGTACTCTGGATCTACAT ACTGCACATGCATTATCACCATTTTCCATTCTGGACAATTCTAATGAAACTGCTTTCCTGATTGGCATTA GTGAAGAGTCCGTGGAAGGCACAGCAGTTTACCTACCAGGACCTGATCTCTGCAAAACAAACCCATGCCT CAACGGAGGCACCTGTTATCCTACCGAGACTTCCTATGTGTGCACCTGTGCACCTGGATACAGCGGAGAC CAGTGTGAACTTGATTTTGATGAATGTCACTCTAACCCTTGTCGGAATGGTGCCACCTGTGTGGATGGTT TTAATACATTTAGATGTCTCTGTCTCCCAAGTTATGTTGGTGCACTCTGTGAACAAGACACTGAGACATG TGACTATGGCTGGCACAAATTCCAAGGACAGTGCTACAAGTACTTTGCTCATCGACGCACATGGGATGCT GCTGAAAGGGAGTGTCGCCTGCAGGGTGCCCACCTCACAAGCATCCTTTCTCATGAGGAACAAATGTTTG TGAATCGTGTGGGCCATGATTACCAGTGGATCGGCCTCAATGACAAGATGTTTGAACATGACTTCCGCTG GACTGACGGCAGTGCACTGCAATATGAGAACTGGAGACCCAACCAGCCAGACAGTTTCTTTTCTGCAGGA GAAGACTGCGTTGTGATCATTTGGCATGAAAATGGTCAGTGGAATGACGTCCCCTGCAATTACCACCTCA CCTACACTTGCAAGAAGGGAACAGTTGCTTGCGGCCAACCCCCTGTTGTAGAAAATGCCAAGACCTTTGG AAAGATGAAACCACGCTATGAAATCAACTCCTTGATTAGATATCACTGCAAAGATGGTTTCATTCAGCGA CACCTTCCAACTATCCGGTGCCTAGGAAACGGGAGATGGGCAATGCCTAAAATAACCTGCATGAACCCAT CTGCATACCAAAGGACTTATTCTAAGAAATACTTAAAAAATTCCTCATCCGCAAAGGACAATTCTATAAA TACATCAAAACATGAGCATCGCTGGAGCCGGAGGCGGCAGGAAACCAGGCGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081249
Insert Size	10065 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081249.1, NP_001074718.1
RefSeq Size	12432 bp
RefSeq ORF	10065 bp
Locus ID	13003

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