Shroom3 (NM_001077595) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AL022960; D5Ertd287e; Shrm; Shrm3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229666 representing NM_001077595 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCAGATATCTCAGGGCACAATGGGCCCTCCTTGGCACCAGAGCTACCATTCCAGCTCTTCGACCA GTGACCTCTCCAACTACGACCATGCTTATCTGAGGCGGAGCCCTGACCAGTGCAGCTCCCAGGGGAGCAT GGAAAGCCTGGAGCCCAGTGGCGGATACCCACCCTGCCATCTCCTTTCTCCTGCCAAGTCCACGAGCAGC ATCGACCAGCTAGGCCACCTTCATAACAAAAGGGACTCCGCATATAGCTCCTTCTCCACCAGTTCGAGCA TCTTCGAGTACCCACCCCCTGGTGGCTCTGCCCGAGAACGTTCGGGTTCCATGGACGTGATTTCTGCCCG GGGTGGCCTCCTAGAAGGGATGAGGCAGGCAGACATCCGCTACGTCAAGACGGTCTATGATACCCGGAGA GGAGTATCTTCAGAGTATGAGGTGAACCCTTCAGCCTTGCTCCTTCAAGGTAGGGATGCCCATGCCTCAG CCGACAGTCAGGGCTGTGCTAAATGGCACAGCATTCCTCGGGGCAAGGGAACGCCTTCCCCGTCTTGGAG CCAGCAGTGTTCTGGTTCCCTGGAGACTGCTACTGACAACCTGCCCCAGAAGGCAGGCGCACCCCTGCCC CCCACCCGCAGTGATAGCTATGCAGCCTTCCGCCATCGCGAGCGTCCCAGCTCCTGGTCCAGCCTTGATC AGAAAAGGTTCTGCCGGCCTCAGACAAACTCTTCAGGCTCCCAGAAAACCCCATTCGCTGAGGACCAGCT GCATACAGTCCCAGAGAGGAGTCCAGAGAACAGCCCCCCAGTGAAATCTAAGCATAATTATACCCAGAAG GCGCAGCCTGGCCAACCTCTGCTGCCCACTGGCATCTATCCGGTACCTTCTCCGGAGCCTCACTTTGCCC AGGTGCCCCAGCCCTCTGTGAGTAGTAATGGCACCGTCTACCCTGCGCTAGTCAAAGAGAGTGGATACAC AGCTGCTCAGGGAACCTGTAACAAGATGGCTACCTTAGATGAGAACGGGAATCAAAATGAAGCTAGCAGG CCTGGGTTTGCCTTCTGTCAGCCCCTAGAACACAACTCGGTGACTCCGGTGGAGAAAAGGCCAGAACCCA CGGCCAAATACATCTACAAAGTCCATTTTTCTTCAGTGCCTGAAAATGAAGACTCCTCCCTAAAGAGGCA CATCACACCTCCCCACGGCCATAGTCCATATCCCAGCGAGAGAAAGAACATCCACGGTGGCAGCAGAGCG TGTTCCAATCACCACAGTCTCTCGTCCCCCCAGGCCCAGGCCCTGCACGTGGGTGATGACAGGAGGCCTT CCAGGCTCTCTCAGCCCTGGGAGGGTGACTTTCAGGAAGATCACAATGCCAACCTCAGGCAGAAAGTGGA GAGGGAGGGCCAAGGCCAGGGCCTGTCGGGCAACTCTGGCAGGACCAGATCGGCCTTCTCATCTCTGCAG AATATTCCAGAGAGTCTGAGAAGACAAAGCAACGTGGAGCTTGGAGAAGCCCAGGAGGTTCACCCCGGGG GCAGGTCCAAGGTGGAGGACCCTGGAAGGAAAGCAGGGGCTTCAGACATCAGGGGCTACCTGGACAGATC TGTTTCCTATCCGAGGCCCGAGGGGAAAATGAACGCCGTAGACTCCGTCCACAGTGCAGACTCCAGGTAT GAGGAGTCCCCGGCCCCAGCGCTGCCACAGACATCCGGTGCGAGCCAGCGGCGGCTCAGCTCCAGCAGCT CCGCCGCCCCGCAGTACAGGAAGCCCCATTGCTCCGTGCTGGAGAAGGTTTCTAGAATAGAGGAACGAGA GCAGGGGCGCCACAGACCCCTGAGCGTGGGCAGCTCTGCATACGGCCCCGGCTACAGGCCTGGGAGGACC GGCCCGACCCCCAGTACTTCCAGCAGTGACCTCGACGATCCAAAAGCCGGCAGCGTCCATTTCTCGGAGT CCACTGAACACCTGCGCAATGGGGAGCAGAACCCCCCAAACGGGGAGGCTAAGCAGGAAGAGGCTTCCCG GCCCCAGTGCAGCCATTTGATCCGGAGGGCCCCTGCGGACGGCCGTGGACCTCCTGCCCGAGGCGGCGAG CCCTCAAGGCCGGAGGCCCGCCTGCTCCGCAGCCAGAGCACCTTTCAGCTCTACAGTGAGGCGGAGAGGG AAGCCTCGTGGTCAGAGGACAGGCCCGGTACCCCTGAGTCGCCCCTGCTCGATGCGCCTTTCAGCCGCGC CTACAGAAACAGCATCAAGGATGCCCAGTCCCGCGTCCTGGGGGCCACGTCGTTTCGTCGCCGAGACCTG GAACCGGGCACCCCCGCCACCTCGAGGCCCTGGCGCCCGCGACCCGCCTCCGCCCACGTGGGGATGCGGA GCCCCGAGGCCGCGGTGCCCTCGTCCTCCCCGCACACCCCTCGGGAGCGACACAGCGTGACCCCGGCCGC GCCCCAGGCTGCCCGCCGAGGCCCACGCCGGCGCCTCACTGTCGAGCAGAAGAAGCGCTCCTACTCGGAG CCCGAGAAGATGAACGAGGTAGGGGTCTCGGAGGAGGCCGAGCCCACGCCCTGCGGCCCGCCGAGGCCCG CGCAGCCGCGCTTCTCGGAGAGCACCGTGGCCGACCGGCGCCGCATCTTCGAGCGCGACGGCAAGGCCTG CTCCACGCTCAGCCTGTCGGGCCCCGAGCTCAAGCAGTTCCAGCAGAGCGCCCTGGCCGACTACATCCAG CGTAAGACGGGCAAGCGGCCCACAGGAGCCGCCTGCACACCGGAGGCCGGGCTGCGCGAGCGCGCACAGA GCGCCTACCTCCAGGCCGGCCCCGCGGCGCCCGATGGCCCCGGCCTGGCCTCTGCCTGTAGCCTGAGCTC CCTGCGAGAGCCCGAGGCGCTGCCCCGCAAGGAGCACACTCATCCGTCGGCCGCGGACGGCCCGCAGGCG CCCCGTGATCGCAGCAGCTCCTTCGCCAGCGGACGCCTAGTCGGGGAGCGACGTCGCTGGGACCCTCAGG TCCCCAGGCAGCTGCTCAGCGGAGCGAACTGCGAACCGAGGGGCGTCCAGAGGATGGACGGGGCCCCGGG CGGGCCGCCTTCCTGGGGCATGGTGGCCGGGAAGGCCGGGAAGTCCAAGTCAGCCGAGGATCTGCTGGAG CGCTCCGACACACTGGCGGTCCCTGTGCATGTGAGGTCCAGGTCGTCTCCCACCTCCGACAAGAAAGGTC AGGATGTGCTCCTGAGGGAAGGCAGCAACTTTGGTTTTGTAAAGGATCCATGCTGTTTGGCTGGCCCTGG ACCTAGGTCTCTCAGCTGCTCAGACAAAGGCCAGAATGAGCTGGCATTGCCCCTGCACCACCCTACGCCC TGCTGGAACGGCTCAGGCTGCAAAGCCACTGTGGCTTCCTCAGCTCCTCCCGAATCTTCTGGAGCCGCGG ATCATCTGAAGCAACGTAGAGCGCCTGGTCCTAGGCCACTCTCAGCAGGAATGCACGGCCACTTCCCAGA CGCCAGAGCTGCCTCACTCAGCTCCCCTCTGCCATCACCTGTCCCCTCCAGTTACCGGTCACAGCTTGCC ATGGATCAGCAGACTGGGCAACAGCCTCCCTCATCCCCTGCCTCTGCAGTGACCCAGCCTACCAGTCCAA GAAGCCCTGAGCTCAGCAGCCCAGCATATGGGCTAGGGGAGGGGATGTGGAAGAGGACATCCCTGCCTCA GCGACCCCCTCCCCCTTGGGTGAAATGGGCCCACGCGGTCAGAGAGGACGGCCTTGCTGAGGACACCTTG GCGCCTGAGTTTGCGAACCTGAAGCACTATAGAAACCAGCCGAGTCGTCCGAGTTCCTGCAGCACTTCTG ACCCAGACACTCCAGGAAGGATCTCGCTCCGAATATCGGAGTCAGCCCTTCAACCTTCCCCACCGCCCCG GGGGGACTATGATGACGAAGTGTTCATGAAGGACTTGCACCCCAAGGTTACTTCAAGCCCCACATTTGAA GCTCTTCCCCCACCCCCGCCACCTTCGCCACCGAGCGAGGAACCTCTAGTGAATGGCACAGATGACTTTC CTCCGCCTCCTCCTCCCCAAGCCCTGTGTGAGGTACTGCTGGATGGTGAGGCATCCACGGAAGCTGGCAG CGGCCCCTGCAGAATTCCCAGGGTGATGGTCACAAGGGAAGGGCACGTGCCTGGGGCAGCCCATTCCGAA GGTAGTCAGATAATGACTGCCACACCACCCCAAACCTCGGCCAAAGGTTCAGAAGCCGAGTCCAACACGC CTTCCAGCGCGAGCGCCCAGCCTCAGCTCAACGGTTCTCCTGGCAAGCAGCTCTGTCCCAGCCAGACCCG AAACCTCACTTATGAACCTGTCGAGAGAACTCAGGATCTAGGAAAGAAAACTCACGCCGAGCCTCAGAAG ACCTCAGAAGATATCCGGACAGAGGCTCTAGCCAAGGAAATTGTCCACCAAGACAAGTCTCTGGCAGACA TTTTAGATCCTGACTCCAGGATGAAGACAACGATGGACCTGATGGAGGGTTTATTCCCTGGAGACGCCAG TGTGCTGATGGATAGTGGCGCAAAGAGGAAAGCCTTAGACATCACCGCCAGGCGTGCGGGATGTGAAGCC AAGGCGAGCGACCACAAGGAGGCAGTGAGTGTGCTGGTCAACTGTCCTGCCTACTACAGTGTCTCTGCTG CCAAGGCGGAGCTGCTCAACAAAATCAAAGACATGCCGGAGGAGCTGCAGGAGGAGGAGGGGCAGGAGGA TGTCAATGAGAAAAAGGCTGAGCTTATCGGAAGCCTTACCCACAAGCTGGAGAGCCTCCAGGAAGCCAAG GGGAGCCTGCTCACGGACATCAAGCTGAACAATGCCCTGGGAGAGGAGGTGGAGGCTCTGATCAGTGAGC TCTGCAAACCCAACGAGTTTGACAAGTACAAGATGTTCATAGGGGACCTGGACAAGGTGGTCAACCTGCT GCTGTCCCTGTCTGGACGCCTGGCCCGCGTGGAGAACGTCCTCAGAGGCCTTGGTGAAGACGCCAGCAAA GAGGAAAGGAGTTCTCTGAACGAGAAAAGGAAGGTTCTGGCTGGCCAGCATGAGGATGCACGGGAGCTCA AGGAGAACCTGGACCGGAGGGAGCGCGTGGTGCTGGATATCCTGGCCAACTACCTGTCCGCCGAGCAGCT GCAGGACTACCAGCACTTCGTGAAGATGAAGTCCACACTCCTCATTGAGCAGCGGAAGCTGGATGACAAG ATAAAGCTGGGCCAGGAGCAGGTCAGGTGCCTGCTGGAGTCGCTTCCCTCGGACTTCAGGCCCAAGGCAG GGGCCATCTCCCTCCCTCCAGCCCTCACCGGCCATGGGACTCCTGGGGGGACATCTGTATTCGGTGGTGT TTTCCCAACGTTGACCTCTCCTCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001077595
Insert Size	5418 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001077595.2, NP_001071063.1
RefSeq Size	6838 bp
RefSeq ORF	5418 bp
Locus ID	27428
UniProt ID	Q9QXN0
Gene Summary	Controls cell shape changes in the neuroepithelium during neural tube closure. Induces apical constriction in epithelial cells by promoting the apical accumulation of F-actin and myosin II, and probably by bundling stress fibers. Induces apicobasal cell elongation by redistributing gamma-tubulin and directing the assembly of robust apicobasal microtubule arrays.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2).

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