Tdrd7 (NM_001290475) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730495N10Rik; AI447470; PCTAIRE2BP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229386 representing NM_001290475 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGCCAGCAGTCTACAACCCAGAGGAGGGTCCTCAGCAGAACCGGACCAAGCTGGCACCCGGATC CTGGACTTTCAGCCTCCAGAACCGCAAAGATGCTGGAGGCAGATCTGGTTTCAAAGATGCTAAGGGCTGT CCTGCAGTCTCATAAGAACGGGATCGTATTACCCCGTCTCCAAGGAGAGTACCGATCTTTGACTGGAGAC TGGATCCCCTTCAAGCAGCTGGGCTACCCTACTTTGGAAGCCTACCTGAGAAGCGTGCCAGCAGTTGTCA GGATAGAGGCTAGTAGATCTGGAGAGATCGTCTGCTATGCAGTAGCCTGCACAGAAACTGCAAGGATCGC TCAGCTTGTGGCTCGTCAGAGGACCTCTAAAAGGAAAATAGGGCGACAAATTAATTGTCAGATGAGAGTG AAGAAAGCCATGCCGTTCTTTCTAGAAGGAAAGCCAAAGGCAACTCTCAGACAACCAGGATTTGCCTCAG ATTATTCCATCAGCAGGAAACCTAATTCAGCGCTGTTAAGAGACAGAGGAAGCGCCTTGGGAGTTAAGGC TGACGTGGACATGCCGCCGTACCCAGACACTCCAGTGCAGAGGCACGCGAGCATGTCTGCCAATAGCAGG TTCAGTCCAAAGTCATCCTTGCCAGCGTCTTTCCAGACACACATCTCAAGGGCCTGCCCTACGGAAGTCA ATGATAATTTAAATCAGACTGTTGAGAAACCAAATATCACGCCTCCTGCCTCTTACACTAATAAGATGGA TGAGGTTCAAAACCGCATAAAGGAAATATTAGACAAGCATAACAATGGCATTTGGATATCTAAGCTTCCA CATTTTTACAAAGAGTTTTATAAAGAAGACCTTAACCAAGGAGTTTTACAGCAGTTTGAACACTGGCCTC ACATTTGCACGGTGGAAAAACCTTGTGGTGGTGGTCAAGATTCACTTCTTTATCCTGCCAGGAGAGAGCA GCCCCTGAAAAGTGACCAGGACCCTGAGAAGGAGCTCCCACCTCCACCTCCTGCTCCCAAGCAGGAGGTT CCATCCCAAGGGAGCCCAGCAGTGATGCCAGATGTCAAGGAGAAAGTGGCAGAACTGCTGGGCAAGTACT CAAGTGGCCTTTGGGCAAGTGCGCTCCCCAAAGCATTTGAGGACATGTACAAAGTCAAGTTCCCTGAGGA TGCCTTAAAAAATCTCGCCTCGCTCTCTGATGTGTGCACCATAAACTACATTTCTGGAAACACCCAGAAG GCAATTCTCTATGCGAAACTCCCATTGCCCACTGACAAGATCTTAAAGGATGAAGGGCAGGCACAAGGAG ATTTTGACATCAAGTCGATGATTGAACAAGAGTATTTGCAGATAGAAAAAAACATGGCTGAAAGTGCTGA TGAGTTTCTGGAGGACATAACTGTGCCGCCTCTGGTAATTCCTACCGAGGCCTCACCGTCTGTGTTGGTG GTTGAGCTGAGCAACACAAATGATGTGGTTATCAGGTATGTAGGCAAAGACTACTCAGCTGCTCAGGAAC TGATGGAGGATGAGATGAAGGAGTTTTACAGTAAGAACCCCAGAGTCACACCGATCCAGACTGTGCACGT GGGGCAGCTGCTGGCAGTCAATGCAGAGGAGGATGCCTGGTTGCGAGCACAGATCATTTCCACGGATGAG AACAAGATAAAGGTGTGCTATGTTGACTATGGCTTTTGTGAAAACATTGAGAAGAGTAAAGCGTACAGAC TGAACCCACGGTTCTGCTCGCTTTCCTTTCAAGCCACCAAGTGCAAGCTGGCAGGGTTGGAGGTTCTGAA TGATGACCCTGACCTTGTGAAGGCGGTGGAATCACTAACATGTGGGAAGATCTTTGCTGTGGAGATCCTG GACAAGTCAGATGTCCCGCTGGTGGTGCTGTACGACACGTCAGGAGAGGACGATATCAACATCAATGCCA CCTGCCTCAAGGCAATATGTGACAGATCTCTACAAGTTCATCTCCAGGTGGATGCCATGTACACAAATGT CAAAGTGACCAACATTTGCTCTGATGGAACACTCTACTGCCAGGTGCCCTGCAAGGGTCTGAATAAACTC AATGACCTTCTGCATAAGACAGAGGACTACTTCCACTGCAAGCACATGACCTCTGAGTACTTCATCTCGC TACCCTTCTGTGGGAAGATCTGCCTCTTCCATTGCAAAGGGAAATGGTTACGAGTGGAGATCACTAATGT TCATAGCAGCCGTGCACTCGACGTTCAGTTCCTGGACTCTGGCAATTCGACATCCGTGAAAGTGTCAGAA CTCAGAGAAATCCCACCGCGGTTCCTGCAGGAAATGCTTGCCATACCCCCTCAGGCTATAAAGTGCTGCT TGGCTGATCTTCCACAGTCCATTGGCATGTGGACACCAGATGCTGTTCTGTGGTTAAGAGATTCTGTTTT GAATTGCTCGGACTGTAGTATTAAGGTTACAAAAATGGATGAGACCAAGGGGGTTGCCTATGTGTACTTA TTCACTCCCAATAACTTCCCGGATCCCCATCGCAGTATTAACCGGCAGATCACAAACGCAGACCTGTGGA AACATCAGAAGGATGTGTTTCTGAGCGCGGTGTCTACTGCGGCCAGTTCCCCTGGCAACAGGAATGGTGG CACACCTGCTCCGGGCAGCCCTGCAGAGAGTCTCAGAAAGAGCCACCCAGAGGTTATCAAGAAGTCTGTC CTGGACCATACCAGCTCTTTCTCCTTGGAGGAACTGCCCCCTCCTGTCCACCTGTCAAGGTCAGGGGAAC ACATGGATGTGTATGTGCCTGTGGCCTGTCACCCAGGCCATTTTGTCATCCAGCCCTGGCAGGAGATCCA TAAGCTAGAAGTGCTGATGGAAGAGATGATCCTGTACTACAGCGTGTCCGAGGAGCGCCACATAGCGGTG GAGCGAGACCAAGTGTACGCAGCCAAAGTGGAGAATAAGTGGTACAGGGTACTTTTAAAAGGAATCCTGA CCAATGGGCTGGTGTCTGTGTACGAGCTGGACTACGGCAAGCATGAGCTCGTCAACATAAGGAAAGTGCA GCCCCTAGTGGACGTGTTCCGAAAGCTGCCCTTCCAGGCGGTCACCGCTCAACTGGCAGGCGTGAAGTGC AGCCAGTGGTCGGAGGAGGCTTCGATGGTGTTTCGAAATCACGTGGAGAAGAAAGCCCTGGTGGCGTTGG TGCAGACAGTTGTTGAGCACACTAATCCTTGGGACCGGAAAGTGGTGCTCTATCTCGTAGACACGTCGCT GCCCGACACTGACACCTGGATTCATGACTTTATGTCACAGTATCTGCTAGAGCTTTCGAAAGTTAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290475
Insert Size	3360 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290475.1, NP_001277404.1
RefSeq Size	3658 bp
RefSeq ORF	3360 bp
Locus ID	100121
UniProt ID	Q8K1H1
Gene Summary	Component of specific cytoplasmic RNA granules involved in post-transcriptional regulation of specific genes: probably acts by binding to specific mRNAs and regulating their translation. Required for lens transparency during lens development, by regulating translation of genes such as CRYBB3 and HSPB1 in the developing lens. Also required during spermatogenesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1).

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