Lpin3 (NM_001199118) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9130206L11Rik; AA438110; AV236139; mKIAA4023
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229040 representing NM_001199118 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTATGTAGGGCAGCTGGCAGAGACAGTGTTTGGAACGGTTAAGGAGCTGTACAGGGGCCTGAACC CGGCCACACTGAGCGGCGGCATCGATGTGCTGGTGGTAAGGCAGCGGGACGGCTCCTTCCGATGCTCACC CTTCCACGTGCGCTTCGGCAAGCTGGGTGTCCTGCGGTCACGGGAGAAGGTGGTGGACATTGAGATCAAT GGTGAGCCCGTGGACCTGCATATGAAACTGGGGGACAGCGGCGAGGCCTTCTTTGTCCAGGAGCTGGACA GTGATGAGGAAGATGTGCCTCCTCGTCTGTGCACCTCACCCATCCCATGGGGAGGCCTGTCGGGCTTTCC CTCAGACTCCCAGATAGGCACAGCCAGTGAGCCTGAGGGCCTAGTCATTACAGGGCGGAGAAAGAGGCGG CGAAGGAGGAAACCGAGGCGCAGGGAGGAGGATGCCGTGGACTCCAGTTCTGAAGAGTTGGAGGCAGGTG CGGAGAGTGAGCTGACATTGCTGGAAAAGCCAACACCAGAGTCCCCAAGTGCTCAGGAGGCAGAGGAGCC CTCATCGCAGCCTAAGGACATACACCCCTACTCCGATGGCGAGTGTACCCCACAGGCCAACCTCTCCTCT GGTGACCTAATGTCCCCTAAGAGTGACTCAGAGCTAGAGCTGCGGTCCTTGGAGCCCAGTCCTCTGAGAG CTGAGTCTCACATGCAGTGGGTCTGGGGCAGACTGCCTAAGGTGGCGAAAGCTGAACGGCCTGAGTTCTC GTTGATCCTTGAAAGTATGGCTGAGGCGATCTGTGCTCTTCCTGAGGAGCCTAGCCCTTCCTCCTCTCCC TCTGAGGCTGGTGTGGACACTTTGAGTCCCCCGGTGCTGCACCCAGGAGTCAGGGCTGATACATTCCATC CTGCTGTGGAGGCCCATTGTGAGGAAACTGCAGTGGATTCTCCTCTTGCTGCCCCAGAGAGTAAGGAAAC CAAGACTCAGAACTCCAGGGGTGCAGGCCACCCTCCTGCCACTAAATCATGGAGCTGGACTACTCCAGAG AGCCATACTCCCTCTGGGCATCCACAAGTTTCCAGGGGAAAGGGTTCCCTGAAGAGAAACCAGCACTTGG GCCCAAGTGACATCTACCTGGATGATCTACCCTCCCTGGACTCTGAGAACGTAGCCCTTTATTTCCCCAA GAGTGAGTATGGGATGGGGGCCAGAAGGTGGAGTGAGCCCAGCAACCAGAAGCTCCTGGAGAGCCCCAAC CCTGAGCACATAGCAGAATGCACTCTGGACTCAGTGGACAAAATAGAATTGTCCCTTTGTGGTGGACTGG CTGACAACCGAGATATCTCCCTGGAGAAGTTCACCCAGCACATGGTCTCCTATGAAGACCTCACTAAGAA TCCCGGGCTCCTGGATGACCCAAACCTCGTGGTGAAAATCAACGAGAAGCATTACAACTGGGCTGTGGCT GCGCCCATGATTCTTTCTCTGCAAGCCTTCCAGAAGAACTTACCTGAGAGCACTGTGGACAAGCTGGAGA AGGAGAAGATGCCCCGGAAGGGTGGGCGATGGTGGTTTTCCTGGAGACGCAGGGACTTCCCAGCTGAGGA GCACAGTTCCCAGAGGGAGAAAGCCGCAACCAGGAAACAGCAGGGAGAGAAGACAGAAGTCTTAAGCAGC GATGACGATGTCCCAGACAGCCCTGTGATCCTTGAAGTCCCCCCACTGCCATCCTCCACTCCAGGCTATG TCCCGACCTACAAGAAGTCTCTTCGTCTCTCTTCTGATCAGATTCGGTGTCTAAACCTGAACGAAGGAGC TAACGATGTGGTCTTCAGTGTGACGACCCAGTACCAAGGCACCTGCCGTTGCAAAGCCACCATCTACCTG TGGAATTGGGATGACAAGGTGGTCATCTCTGACATAGATGGCACCATCACCAAGTCGGATGCTCTGGGCC ACATTCTGCCCCAGCTGGGGAAAGACTGGACACACCAGGGCATTACTAGTCTCTACCACAAGATCCATCT AAATGGGTACAAGTTCCTGTACTGCTCAGCACGGGCTATCGGCATGGCTGACCTCACCAAGGGGTACTTG CAGTGGGTGAGCGAGCATGGCTGTGGCCTTCCTAAGGGCCCCATCTTGCTATCTCCCAGCAGCCTCTTCT CCGCTCTCCACAGGGAGGTAATCGAGAAAAAGCCGGAGGTGTTCAAGGTTGCCTGCCTGAGTGATATCCA GCAGCTGTTTCTGCCCCAAAGACAGCCCTTCCATGCTGCCTTTGGGAACAGGCCCAATGATGTTTTCGCC TACAGGCAGGTGGGCCTCCCTGAGTCCCGAATCTTCACAGTCAACCCCCGGGGGGAGCTCATCCAGGAGC TCATAAAGAGCCACAAATCCACGTACCAGCGGCTCGGAGAGGTGGTTGAGCTGCTCTTCCCACCTGTGGT GCGCGGTCCCAGCACAGACCTGGCCAGCCCTGAATACAGCAACCTCAGCTATTGGCGGAAGCCACTGCCG TATGTGGACTTTGAGGCCCTGGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199118
Insert Size	2547 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199118.1, NP_001186047.1
RefSeq Size	3396 bp
RefSeq ORF	2547 bp
Locus ID	64899
UniProt ID	Q99PI4
Gene Summary	Regulates fatty acid metabolism. Magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

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