Elmo2 (NM_001302754) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1190002F24Rik; CED-12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228759 representing NM_001302754 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCCTCCGTCTGACATTGTCAAAGTGGCCATCGAGTGGCCAGGTGCTAACGCCCAGCTCCTTGAAA TTGACCAGAAACGGCCGCTGGCATCCATCATCAAGGAGGTGTGCGATGGGTGGTCACTGCCGAACCCGGA GTACTACACCCTCCGCTATGCAGATGGGCCTCAGCTCTACGTCACGGAGCAGACCCGAAATGACATTAAG AACGGGACAATCTTACAACTGGCCGTCTCCCCGTCCCGGGCTGCACGCCAGCTGATGGAAAGGACCCAGT CATCTAGTATGGAGACCCGGCTGGATGCCATGAAGGAGTTGGCTAAGCTCTCAGCTGACGTGACTTTCGC CACGGAGTTCATTAACATGGACGGCATCATTGTGCTGACGAGGCTCGTGGAGAGTGGGACCAAGCTCCTG TCCCATGAGATGCTGGCATTCACCCTGACTGCCTTCTTAGAGCTCATGGATCATGGCATTGTCTCCTGGG ACATGGTTTCAGTCACCTTTATTAAGCAGATTGCAGGGTACGTGAGCCAGCCCATGGTCGATGTGTCCAT CCTCCAGCGGTCCCTGGCCATCCTAGAGAGCATGGTACTAAACAGCCAGAGCCTGTACCAGAAGATAGCG GAGGAGATCACCGTGGGACAGCTCATCTCCCACCTGCAGGTCTCCAACCAGGAGATCCAGACCTACGCCA TTGCTCTGATTAACGCGCTGTTCCTGAAGGCCCCCGAAGACAAGAGACAGGACATGGCCAATGCCTTTGC ACAGAAGCACCTTCGGTCCATAATCCTGAACCATGTGATCAGAGGGAATCGTCCAATCAAAACAGAGATG GCCCATCAGCTGTATGTCCTTCAGGTCTTGACCTTTAACCTTCTGGAAGAAAGAATGATGACCAAGATGG ATCCCAATGACCAGGCTCAGAGAGACATTATATTTGAACTGAGGAGGATTGCCTTCGACGCAGAGTCTGA CCCCAGCAACGTCCCCGGGAGTGGGACTGAAAAGCGCAAGGCCATGTATACCAAGGACTATAAAATGCTG GGCTTCACCAACCATATCAACCCAGCCTTGGACTTCACCCAGACTCCTCCTGGAATGCTGGCGCTGGACA ACATGCTGTACCTGGCTAAAGTCCACCAGGACACCTACATCCGGATCGTGCTGGAGAACAGCAGTCGGGA GGACAAACACGAGTGTCCGTTCGGCCGCAGTGCCATCGAGCTCACCAAGATGCTCTGTGAGATCCTGCAG GTCGGGGAGCTCCCTAATGAAGGGCGCAATGACTACCACCCCATGTTCTTCACCCACGACCGAGCCTTCG AGGAACTCTTCGGGATCTGCATCCAGCTGCTGAACAAGACCTGGAAGGAGATGAGGGCGACAGCCGAGGA TTTCAACAAGGTTATGCAAGTTGTCCGAGAGCAGATCACCCGGGCTCTGCCCTCTAAACCCAACTCTTTG GATCAGTTCAAGAGTAAACTTCGTAGCCTGAGCTACTCAGAAATTCTGCGGTTGCGCCAGTCTGAGAGGA TGAGCCAGGATGATTTCCAGTCCCCACCAATTGTGGAGCTTCGAGAGAAGATACAGCCTGAGATCCTGGA GCTGATCAAGCAACAGCGCCTCAACCGGCTATGCGAGGGCAGCAGCTTCCGGAAAATCGGGAACCGTCGT CGGCAAGAGAGGTTCTGGCACTGCCGCTTGGCACTGAACCACAAGGTTTTGCATTACGGTGACTTGGATG ACAACCCTCAAGGGGAGGTGACATTCGAATCCCTGCAGGAGAAAATTCCTGTTGCAGATATTAAGGCCAT TGTTACTGGAAAAGACTGTCCTCACATGAAAGAGAAGAGTGCCCTGAAACAGAACAAGGAGGTGTTGGAA TTGGCCTTCTCCATCCTGTATGATCCTGATGAGACACTGAATTTCATTGCTCCCAACAAGTATGAGTACT GCATCTGGATTGATGGACTCAGCGCTCTCCTGGGGAAGGATATGTCCAGCGAGCTAACCAAGAGCGACTT GGACACGCTGCTGAGCATGGAGATGAAGCTGCGGCTTCTGGACTTGGAGAACATTCAGATCCCCGAGGCG CCACCTCCGGTCCCCAAGGAGCCCAGCAGCTATGACTTCGTCTATCACTATGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302754
Insert Size	2157 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302754.1, NP_001289683.1
RefSeq Size	4538 bp
RefSeq ORF	2157 bp
Locus ID	140579
UniProt ID	Q8BHL5
Gene Summary	Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR, uses an alternate in-frame splice site in the 5' coding region, and lacks an exon in the coding region compared to variant 2. It encodes isoform 4, which is shorter than isoform 2.

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