Fbxw11 (NM_001271349) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310065A07Rik; AA536858; BTRC2; BTRCP2; Fbxw1b; HOS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228095 representing NM_001271349 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGACTCGGTGATTGAAGACAAGACCATCGAGCTCATGATCAGTAATGGAACGTCATCTGTGA TTGTCTCCAGAAAGAGGCCGTCAGAGGGGAACTACCAGAAAGAAAAGGACTTGTGCATTAAGTACTTTGA CCAGTGGTCTGAATCAGATCAGGTGGAATTTGTGGAGCATCTTATCTCACGGATGTGTCATTATCAGCAT GGACATATTAACTCTTACCTGAAGCCCATGTTGCAGCGGGACTTTATCACTGCTTTACCAGAGCAAGGCT TAGATCACATAGCAGAAAACATTCTCTCCTACCTGGATGCCAGGTCTCTGTGTGCAGCAGAGCTGGTGTG TAAAGAATGGCAGCGAGTGATCTCAGAAGGGATGCTTTGGAAGAAGCTGATTGAGAGGATGGTGCGCACC GACCCTCTCTGGAAGGGACTCTCAGAAAGAAGAGGCTGGGATCAGTACCTGTTTAAAAACAGACCTACAG ATGGCCCTCCCAACTCATTTTATAGATCATTATACCCAAAGATTATCCAGGACATAGAGACCATAGAATC CAACTGGCGGTGTGGACGACACAACTTGCAGAGGATCCAGTGCCGCTCTGAAAATAGTAAGGGTGTCTAC TGTTTGCAATATGATGATGACAAAATTATCAGTGGCCTCCGGGACAACTCTATCAAGATCTGGGATAAAA GCAGCTTGGAATGTTTGAAAGTGCTAACGGGCCACACAGGCTCTGTCCTCTGCCTCCAGTATGATGAGCG AGTCATTGTAACTGGTTCTTCAGACTCCACGGTGAGAGTCTGGGATGTGAACACTGGTGAGGTGCTCAAC ACACTCATCCACCACAATGAAGCCGTACTGCACTTACGCTTCAGCAATGGACTGATGGTGACTTGTTCCA AGGACCGTTCCATTGCCGTGTGGGACATGGCTTCTGCCACCGATATCACTTTACGCCGTGTTCTGGTTGG CCACCGTGCTGCTGTCAATGTAGTAGACTTTGATGATAAATACATCGTGTCTGCTTCAGGAGACAGGACC ATTAAAGTGTGGAGCACGAGCACATGTGAGTTTGTCCGCACTCTGAATGGGCACAAGCGAGGCATCGCCT GTCTGCAGTACCGCGACCGGCTTGTTGTTAGTGGATCATCAGATAATACCATCCGGTTATGGGATATTGA ATGTGGTGCCTGTTTAAGAGTCCTAGAGGGGCACGAAGAATTGGTCCGGTGCATCCGTTTTGATAACAAG AGGATTGTCAGTGGCGCCTATGATGGGAAGATTAAAGTCTGGGACTTGCAGGCTGCTCTTGACCCTCGGG CCCCAGCAAGCACATTGTGTCTGCGCACCTTGGTGGAACACTCTGGACGTGTGTTTCGGCTGCAGTTTGA TGAGTTTCAGATCATCAGCAGCTCCCATGATGACACTATTTTGATTTGGGATTTCTTAAATGTGCCTCCC AGTGCCCAGAATGAGACCCGCTCTCCCTCCAGAACCTACACCTATATCTCCAGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271349
Insert Size	1527 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271349.1, NP_001258278.1
RefSeq Size	4174 bp
RefSeq ORF	1527 bp
Locus ID	103583
UniProt ID	Q5SRY7
Gene Summary	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. SCF(FBXW11) mediates the ubiquitination of phosphorylated CTNNB1 and participates in Wnt signaling. SCF(FBXW11) mediates the ubiquitination of phosphorylated NFKBIA, which degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. SCF(FBXW11) mediates the ubiquitination of IFNAR1. SCF(FBXW11) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (By similarity). Involved in the oxidative stress-induced a ubiquitin-mediated decrease in RCAN1. Mediates the degradation of CDC25A induced by ionizing radiation in cells progressing through S phase and thus may function in the intra-S-phase checkpoint. Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and phosphorylated PER2. SCF(FBXW11) mediates the ubiquitination of CYTH1, and probably CYTH2 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks two alternate in-frame exons, compared to variant 1. The encoded isoform (d) is shorter than isoform a.

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