Scyl3 (NM_001286003) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1200016D23Rik; 6030457O16; AW214499; Pace1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228089 representing NM_001286003 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGAGTGTCGGCCAGCACTCAGCACCTTACTGTCCCATGATTTCTTCAGAAATGATTTTCTAGAAG TTGTGAACTTCTTGAAAAGCTTGACATTAAAGAGTGAAGATGAAAAGACTGAATTTTTCAAATTTCTGCT GGACCGAGTGAGCTGCCTGTCAGAAGAATTAATAGCTTCAAGATTGGTGCCTCTTCTGCTTAACCAGCTG GTGTTTGCTGAGCCAGTAGCTGTTAAGAGCTTTCTTCCCTATCTACTTGGTCCTAAAAAAGAGAACGCAC CAGGAGAAACCCCTTGCTTGCTCTCGCCAGCGCTGTTCCAGTCCCGAGTGATCCCTGTGCTTCTCCGGCT GTTCGAGGTGCACGAGGAGCACGTGCGGATGGTGTTACTGTCCCACATCGAGGCCTACGTGGAGCACTTC ACTCAGGAACAGCTAAAGAAAGTCATCCTGCCACAGGTACTACTGGGCCTTCGTGATACCAGCAATTCCA TTGTGGCAATTACTCTCCGTAGCCTGGCAGTGCTGGTGTCTTTACTTGGACCAGAAGTGGTTGTGGGAGG AGAACGAACCAAGATCTTTAAGCGCACCGCTCCAAGCTTCACCAAAACTAGTGACCTTTCCCCTGAAGGT TCTCCCATGCATGTTGTCTGCAGCCAGCAGAGTCGGGTCTCAAAAGTCTTGGAGGACCCCTCGTCTAACG TATTCCCTAAATGGTTGTCTGGCAACGTGCCCAGCAGCAGCAGGAAGCGCATACAGGAAGAATGTTACAG CTCTCTCTCACAGACAGGTGATCAGTTTTCTCACACCATTAAATTCCCTATGAATGGACTCTCAGATGTG AAAAACACTTCAGAGGACAATGGAAGCTTCCCTGCAGGTTCTAATAAGCCCGAGGAGTGGCCTGACTGGA GCGAGCCTGAGGAGCCTGAGCAGCAGCCTGCCAGTATTCACAGATGGCCCCGAGAACCCTGTGATGTAGC AGAGTCCCAGCACACAAACCTGACCGCAGAGGAGGTGACCTGGGATGATGGTGAGGCTAGCTTTGGCACT GAAATAAACTCAACAGCTACAGCCTCTGCTCCTGTGCCTGTTACCTCAGGGGGACAGTCAACCTCTGCAG CTTTGGTTCCTCTCACTGAAGAGTCAAAGCCTTTGCAATCCAGTCCTTCCTCAAAGACCAGTCATAGACA GCATGAGGAAGTCAAACCACCCCAAGTGTCACAAGAAAGACCCCTGAAGGCTCCGTCAGGACTTGGTTTG GGAGAAGAGTTTACCATTCAAGTAAAAAAGAAGCCAGTACAAGACCCGGAGTTGGACTGGTTTGCAGATA TGATCCCAGAAATTAAGCCTTCAGGTACTTTTCTTATTCTGCCTGAACTAAGGACAGAAGTGATGGTCCC TGACAAGGATAACGTCTCATCACTGATGCAGTTTTCCTCAAAGTTTGCTGCAACAGAAATGACCGAGGGA GAGGCAGAAGGCTGGGAAGGAGAAGAGCTAGCCTGGGAAGATAACAACTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286003
Insert Size	1524 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286003.1, NP_001272932.1
RefSeq Size	4411 bp
RefSeq ORF	1524 bp
Locus ID	240880
UniProt ID	Q9DBQ7
Gene Summary	May play a role in regulating cell adhesion/migration complexes in migrating cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate 5' structure and initiates translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 3) has a shorter N-terminus than isoform 1. Both variants 3 and 4 encode the same isoform.

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