Nfib (NM_001286127) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6720429L07Rik; CTF; E030026I10Rik; NF-I/B; NF1-B; NFI-B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228010 representing NM_001286127 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTATTCTCCCATCTGTCTCACTCAGGATGAATTCCACCCATTTATTGAGGCACTTCTTCCTCACG TCCGTGCAATTGCCTATACTTGGTTCAACCTGCAGGCTCGAAAACGCAAGTACTTTAAAAAGCATGAGAA ACGAATGTCGAAGGATGAAGAAAGGGCAGTCAAAGACGAGCTGCTCAGTGAGAAGCCCGAAATCAAGCAG AAGTGGGCATCCAGGCTCCTGGCCAAACTGCGCAAAGATATCCGCCAGGAGTACCGGGAGGACTTTGTGC TTACCGTGACTGGCAAGAAGCACCCGTGCTGTGTCTTATCCAATCCAGACCAGAAGGGTAAGATTAGGAG GATCGACTGCCTGCGACAGGCAGACAAAGTCTGGCGTCTGGATCTAGTCATGGTGATCCTGTTCAAAGGC ATCCCTTTGGAGAGTACGGATGGAGAGCGACTCATGAAGTCCCCGCACTGCACAAACCCAGCACTTTGTG TTCAGCCACACCACATCACAGTATCAGTTAAGGAGCTTGACTTGTTTTTGGCATACTACGTGCAGGAGCA AGATTCTGGACAATCAGGAAGTCCAAGCCACAGTGATCCTGCCAAGAATCCTCCAGGGTACCTCGAGGAC AGCTTTGTAAAATCCGGAGTCTTCAATGTATCAGAGCTTGTGAGAGTATCCAGAACACCCATAACCCAGG GAACTGGAGTCAACTTCCCAATCGGAGAAATTCCCAGCCAACCATACTATCATGACATGAACTCTGGTGT GAACCTGCAGAGGTCGCTGTCTTCTCCACCGAGCAGCAAAAGACCCAAAACTATATCTATAGATGAAAAT ATGGAGCCAAGTCCTACAGGAGACTTTTACCCCTCTCCAAATTCACCAGCTGCTGGAAGTCGAACATGGC ATGAACGAGATCAAGATATGTCTTCTCCAACTACAATGAAGAAGCCTGAGAAGCCACTGTTTAGCTCTAC ATCTCCACAGGATTCTTCCCCAAGATTGAGCACTTTCCCCCAGCACCATCATCCCGGAATACCTGGAGTC GCGCACAGTGTCATCTCAACTCGAACTCCACCTCCGCCCTCACCGTTGCCATTTCCGACGCAAGCTATCC TTCCTCCGGCACCTTCCAGCTACTTCTCTCATCCAACAATCAGATATCCTCCTCACCTGAATCCTCAGGA TACTCTGAAGAACTACGTACCTTCTTATGACCCATCCAGTCCTCAAACGAGCCAGCCTAACAGCAGTGGT CAAGTAGTAGGGAAAGTGCCTGGCCATTTCACACCTGTCTTGGCACCCTCTCCCCATCCCAGTGCAGTGC GACCTGTGACCCTGACCATGACAGATACTAAACCCATCACTACATCCACTGAAGCCTACACAGCCTCAGG CACATCTCAAGCCAATCGATATGTGGGACTAAGCCCAAGAGACCCATCCTTCCTGCATCAGCAACAGTCC TGGTACCTGGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286127
Insert Size	1485 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286127.1, NP_001273056.1
RefSeq Size	9154 bp
RefSeq ORF	1485 bp
Locus ID	18028
Gene Summary	Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate splice site and lacks an alternate exon in the 3' coding region, resulting in a frameshift and an early stop codon, compared to variant 1. It encodes isoform 4, which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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