Ephx2 (NM_001271421) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CEH; Eph2; SEH; sEP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227962 representing NM_001271421 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAAAGCTACAGGAAGTCCTCCAAAGCCTGTGGAGCCAATCTACCTGAGAATTTCTCCATAAGTC AAATATTCAGCCAAGCTATGGCAGCAAGAAGCATCAACCGCCCCATGCTTCAGGCAGCCATTGCTCTCAA AAAGAAAGGATTCACAACATGCATTGTCACCAACAACTGGCTGGACGACGGAGACAAGAGAGACAGCCTG GCCCAGATGATGTGTGAGCTGAGCCAACACTTTGACTTCCTGATAGAGTCCTGTCAGGTTGGGATGATCA AGCCTGAGCCTCAGATCTACAATTTTTTACTGGATACCCTGAAGGCAAAACCCAATGAGGTTGTTTTCCT AGATGACTTTGGAAGTAATCTGAAGCCAGCCCGTGACATGGGGATGGTTACCATCCTGGTCCACAACACA GCCTCCGCTCTGAGAGAACTGGAGAAGGTCACAGGGACACAGTTTCCTGAGGCCCCACTGCCAGTCCCAT GCAATCCAAATGACGTCAGCCATGGATATGTGACAGTGAAGCCAGGGATCCGCCTGCATTTTGTGGAGAT GGGCTCTGGCCCTGCCCTATGCCTTTGCCATGGGTTTCCTGAGAGCTGGTTTTCTTGGAGGTACCAGATC CCTGCTCTGGCCCAGGCAGGCTTTCGTGTTCTGGCTATAGACATGAAAGGCTATGGAGACTCATCTTCTC CTCCTGAAATAGAAGAATATGCCATGGAATTGCTGTGTAAGGAGATGGTGACATTCCTGGATAAGCTGGG AATCCCTCAAGCAGTGTTCATTGGCCATGACTGGGCTGGTGTGATGGTGTGGAACATGGCTCTCTTCTAC CCTGAGAGAGTGAGGGCTGTGGCCAGTTTGAACACGCCGTTTATGCCACCAGATCCTGATGTGTCTCCCA TGAAAGTTATCCGATCTATCCCAGTTTTCAATTATCAGCTGTACTTTCAAGAACCAGGAGTGGCCGAGGC TGAACTGGAGAAGAACATGAGTCGGACTTTCAAAAGCTTCTTCCGAGCCAGTGATGAGACAGGTTTCATC GCTGTGCATAAAGCCACTGAAATAGGGGGAATCCTTGTGAATACTCCAGAAGATCCCAACCTCAGCAAAA TTACTACTGAGGAAGAAATAGAGTTTTACATACAGCAGTTCAAGAAGACTGGCTTCAGAGGTCCTCTGAA CTGGTACCGGAACACAGAAAGAAACTGGAAGTGGAGCTGTAAAGGGTTGGGACGAAAGATCTTGGTCCCA GCCCTGATGGTCACAGCTGAGAAGGACATTGTACTCCGTCCTGAAATGTCCAAGAACATGGAAAAGTGGA TCCCTTTCCTGAAAAGGGGACACATTGAAGACTGTGGTCACTGGACACAGATAGAGAAACCAACTGAGGT GAACCAGATTCTCATCAAGTGGCTGCAGACTGAAGTCCAGAACCCATCAGTGACCTCCAAGATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271421
Insert Size	1467 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271421.1, NP_001258350.1
RefSeq Size	2037 bp
RefSeq ORF	1467 bp
Locus ID	13850
Gene Summary	Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. This variant is considered to be 5' complete based on evidence from PMID: 17900570. The encoded isoform (d) has a shorter N-terminus than isoform a.

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