Arl4a (NM_007487) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI467555; Arl4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226252 representing NM_007487 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAATGGACTGTCAGACCAGACTTCCATCCTGTCCAGCCTGCCGTCCTTTCAGTCCTTTCACATTG TTATTCTGGGTTTGGACTGTGCTGGAAAGACAACAGTTTTATACAGGCTGCAGTTCAACGAATTTGTAAA TACCGTACCTACCAAAGGATTTAACACTGAGAAAATTAAGGTAACCTTGGGCAATTCCAAAACAGTCACT TTTCACTTCTGGGATGTAGGTGGTCAAGAGAAGTTAAGACCACTGTGGAAGTCATACACCCGATGCACAG ATGGTATTGTGTTTGTGGTGGACTCTGTCGATGTTGAGAGAATGGAAGAAGCCAAAACTGAACTTCATAA AATAACTAGGATATCCGAAAATCAGGGAGTCCCTGTGCTTATAGTTGCTAATAAACAAGACCTGAGGAAC TCGCTCTCTCTCTCAGAGATTGAGAAGTTGTTAGCAATGGGGGAACTGAGCTCATCGACTCCTTGGCATT TGCAGCCCACCTGTGCAATCATAGGAGATGGGCTAAAGGAAGGACTCGAGAAACTACATGATATGATAAT TAAAAGGAGAAAAATGTTGCGGCAACAGAAAAAGAAGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007487
Insert Size	603 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007487.3, NP_031513.1
RefSeq Size	3657 bp
RefSeq ORF	603 bp
Locus ID	11861
UniProt ID	P61213
Gene Summary	Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) and variant 2 encode the same protein.

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