Svil (NM_153153) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AU024053; B430302E16Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225209 representing NM_153153 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAGAAAAGAAAGAATTGCTAGGCGCTTAGAAGGAATAGAAAATGACTCCCAGCCCATCCTGTTGC AGAGCTGCACAGGGCTAGTGACTCATCGGCTGCTAGAAGAAGACACACCCAGATACATGCGTGCCACAGA CCCTGCAAGCCCTCATATTGGGCGATCCAAAGAAGAGGAAGACACTCCTGGGTCCTCCCTAGAAAAGCAA ACTCCTTCTAAATACTGCATAGAAACCTCAGGCATCCATAGTTCGGGCTCCATGGACACCCACAGTCTGG AGTCCAAAGCGGAAAGGATCGCAAGATACAAAGCAGAGAGGAGGCGGCAGCTGGCCGAAAAGTACGGGCT GACCTTGGACCCAGAAGCTGACTCTGAATACTTGTCTCGATATGCCAAGTCTCGGAAGGACCCAGATGTG ACTGAGAGACGAGGAAAAAGTGACAAGCAAGAAGAACAGAGCAAGGATGCCAATTCCCGGCACTCCAGGA CAGAGTCAGGGCCCAGGACCAGCTTGGTGGCGTCCCAGGACTGTACCCCGCTGGGAAGCAATATGTCTGA CCAGGAGCAGCTGCTCAATGTGGAGAACCAGAGAAGAGTCCAAGATCCCCCACTAGGAGAAGATGGCTCC TCTGCCTTCTTTTCTGAGCGAAGCATTTCCTTCCCCGAAGTGCCCAGGTCTCCAAAGCAGATACCCAGCT CACCACTGCAACAGCCAGCCTCCCCAAACCACCCCGGTGACTCACCACTGCCCACAGAGGCCCGAGCCAG TACAGGGAAGCCCACACATGAGTGGTTTCTGCAGAGAGATTCTGAAGGCGATACACCTTCACTTATCAAC TGGCCTTCCAGAGTTAAAGTTAGAGAAAAATTGGTGAAAGAGGAGAGTGCTCGCAGCAGCCCTGAACTCA CCTCAGAGTCCTTAACTCAGAGGAGACAGCAGCCGGCACCAGCACATTTCCTGCCTATCCAGTCAGAAAG TTCCACCTTTGATAGGGTCACCAGCAAAGCAGTGAGCTCATTGCAGCCAAGCCAGAGTGGTGTTCTGCCA ACTGACCCTGTTCATGCCATCAAGCTGGTGACCATGGATACCCCAGAAAGCACATCTGAATTCAGCTGGG TGGGGTCAGCCACCCCAAAAGTTATAAAATCTACCACCTTGAAGATTCTAGAAGGTGGTTCAAGGGATGC TCCAGTACTCCATATTTGTGAGTCAAAAGCAGAAGATTGGCTCTCCCCAGAACCTCTGGAGAGGAGCCCC AAGTCACTTTTGACCTCAGAAGATGACAGGCTCGTGAGAGGCCACAAAGATCCCTCTGGTAATAAAGACT TGGACAAGGCCATCATTTGCTCCATCGATGTAGAATCTGAGAGGGAGCGGCAAGTTCAACACCTGCCCAC TCAGAGAACTGGCCGGAGTGAAATGCTCCTGTATGTTCAGAGTGGTCCTGTGTCCCAAGATGCCACATTG ACCAGTCACACAAAGGAGGCATCTCCAAAGAAGCGCAAGGTTCTGGCCCGCTCTCTGTCAGATTACACAG GTCCCCCTCAGCTCCAGGTCCCCAGACACAAGGATGAAGCCCCAAGCCAGGAGCTGGAGCTGCAGAGTTC CAGGGCAGAAGGGCCTGGTGCAGAAGCCAGCGTGCTGGACACCAGAGTCTCCGTCGCCCAGCTCCGAAAC ATATTTATGGAGTCAACCCGAGCCAGCAAGAAACCTGAACTCCAATCCCGGGTTGAGAGGTCAGCTGAAG GAATTGGCTTGCCCATGGAACGAGAGAGAGGGTCTCGGAAACCAAGACGCTATCTTTCTCCTGGTGAAAG TAGAAAAACTTCTGAGCGATTTAGAACTCAACCTATAACCTCAGCAGAGCGAAAGGAGTCGGATAGGTAC CCTTCTGGTTCAGAAATACCAGTGGTCGAGGATGAAGAAAAGGTGGATGAGCGGGCCAAGCTGAGCGTGG CTGCCAAGAGGCTGCTTTTCAGGGAAATGGAGAAATCCTTTGATGAACACACTGTCCCAAAGAGACATTC GAGAAATGCAGCTGTGGAGCAGAGGCTGCGTCGCCTGCAGGACCGTTCACACACACAGCCCATCACCACG GAGGAAGTGGTCATTGCAGCTACTGAACCTATCCCTGCTTCGTGTTCTGGGGTCACCCACCCTGTAACGG CGAGACTTCCTAGCCCCACTGTAGCTAGAAGCAGTGTGCAGCCTGCCAGGTTACAAGCATCTGCTCACCA AAAGGCTTTAGCCAGGGACCAGGCAAATGAGGGCAGAGAGTCTGCCGAACCAGGCGAACCTGATTCCTCC ACTCTGAGCTTAGCAGAGAAATTGGCCTTGTTTAACAAATTGTCCCAGCCAGTCTCCAAAGCCATTTCCA CCCGAAACAGAATAGATGTGCGGCAGAGGAGGATGAACGCAAGGTACCAGACGCAGCCGGTCACTCTTGG AGAAGTGGAGCAGGTGCAGAGTGGGAAACTCATCTCCTTCTCCCCCACTGTGAACACCTCTGTTTCTATT ATGGCCTCTGCGGTTGCTCCCACATATGCAGGGGACCTTCGGAAGCTATCTGTTGACAACAACACAAGTG CCACTGACTATAAGTCTCCTCCTGCGGAAAACTCAGACTCTCCAGTCAGAAGCATTCTGAAACCACAAGC TTGGCGGCCTTTGGTAGAGCACAGTGGGAGCAAAGGAATGCCGGGGGAGTCTGGAAAGACAGAAAGCAAG AATGCTCTGACTGTGGCTGCAGAAGACAGTGGCGTTCAAACACGAGGAGCCTTCGAGGAGGAAGAGGAGC CCTCCTACCCCATCCTTGGCAGAGTCAGGGAGGGTGATGGCCAGAAGGAGCCTAAGCATGTCGTCCTAAG GAGGGGAAGCTTAGAGCTAGGCAATCCTTCTGCTGCACACCTTGGTGATGAGCTGAAGGAAGTTTCCACT GCTAAAAGCAGTTTGCAAGAGAATCTAGACCTGAAGGATAAGCAGGCCTCAGAGGAGAACACAGATGTGG AAACTGTCATGAGGAAGTTCTCACTGAAAGAGTTTGGAGAAACGACTTCTGAGCAGACAGAGGTGGCTGC CAGGAAGGCCTCGGTTCAGATGGCGACCCCAGGGGCCTGGAAGCAGCAGGAGTCCTCTGAGCAACTAGCA GAGAAGCTCTTCAAGAACCCTTGTGCCATGTTTGCTTCTGGAGAGGTCAAGGTGCCAGTAGGCGACAGTT TCCTGGATTCACCCAGCAAAACCATGTCAATCAAAGAAAGGCTGGCACTGTTAAAGAAGAGTGGCGAGGA AGACTGGAAGAACAGGCTGATTCGGAAGCAGGAATATGGCAAAGCCACTGGCGGCCTGCACACGCAGGAG GTGGAGCAGTCCCTGAAGAAAAAGAGAGTCACAGAAAGTCGAGAGAGCCAGATGACGATTGAAGAGAGGA AGCACCTCATCACTGTGCGCGAGGAAGCGTGGAAGACGAAGGGCAGAGGCGCTGCCAATGACTCCACACA GTTCACCGTGGCAGGAAGAATGGTGAAGAAAGGTCTGGCATCACCCACATCCATAACTCCCATCTCATCC CCTCTCTGCAGTAAATCAAGGGGCACTACCCCGGTTTCCAAACCCTTGGAAGATATTGAAGCCAGACCAG ATATGCAGCTGGAATCAGACCTGAAGTTGGACAGACTGGAAACCTTCCTAAGGAGGCTAAATAACAAGGT TGCTGGGATACAGGAAACGGTTCTCACGGTCACTGGCAAATCTGTCAAGGAGGTGATGAAGTTGGATGAT GATGAAACATTTGCCAAATTCTACCGCAGCGTGGATCACAGTATACCCAGAAGTCCTGTGGAGCTGGAGG AGGACTTCGATGTCATTTTCGACCCTTATGCTCCCAAGTTGACATCTTCTGTGGCAGAGCATAAGCGCCA GGTCAGGCCCAAGCGCCGGGTCCAGGCTTCCAAGAACCCTCTCAAGCTGCTGGCAGCAAGAGACGACCTC CTTCAGGAATACACAGAGCAAAGGCTAAATGTGGCCTTCATGGAGTCCAAGCGGATGAAAGTGGAAAAGA TGTCATCCAACTCCAACTTCTCAGAAGTGACTCTGGCAGGCCTGGCCAGTAGGGAGAACTTCAGCAACAT CAACCTGAGGAGTGTCAATCTGATGGAGCAAAACTCCAACAACAGCGCGATGCCCTACAAGAAGCTCATG CTGTTGCAGATTAAAGGAAGAAGACATGTGCAGACAAGGCTGGTGGAGCCCCGAGCCTCATCACTCAACA GTGGGGACTGCTTCCTTCTGCTGTCTCCTCAGTACTGCTTCCTGTGGGTGGGAGAGTTCTCCAATGTCAT CGAGAAAGCAAAGGCATCTGAACTTGCAACGTTAATTCAGACAAAGAGGGAGCTTGGTTGTAGAGCTACA TACATCCAGACCATCGAAGAAGGAATTAACACACATACTCACGCAGCCAAAGACTTCTGGAAGCTCTTGG GTGGCCAGACCAGTTACCAGTCTGCCGGAGACCCAAAGGAAGATGAGCTGTACGAGACAGCCATCATAGA GACGAACTGTGTCTACCGCCTGACAGATGACAAACTCGTTCCTGATGATGACTACTGGGGGAAGATTCCC AAGTGTTCCCTTCTGCAGTCCAAAGAGGTCCTGGTGTTTGACTTTGGTAGTGAAGTTTACGTGTGGCATG GGAAGGAAGTCACGTTAGCACAACGGAAAATAGCATTCCAGCTGGCTAAGCACTTATGGAACGGAACCTT TGACTATGAGAACTGTGACATCAACCCCCTGGACCCCGGAGAGTGCAACCCGCTCATTCCCAGGAAAGGA CAAGGACGGCCTGACTGGGCAATATTTGGAAGAGTTACGGAGCACAATGAGACTATTCTGTTCAAAGAGA AATTCCTTGACTGGACAGAGCTGAAGAGACCCACAGAGAAGAATTCTGGAGAAGTTGTCCAGCAGAAGGA TGACCCGAGGGCCGACGTCAAGCCTTATGATGTGACACGGATGGTGGCGACGCCCCAGATTACAGCTGGC ACCATCTTAGATGGGGTGAACGTTGGCCGTGGCTATGGCCTGGTGGAAGGAGATGACAGAAGACAGTTTG AGATTGCCACTGTCTCTGTGGATGTGTGGCACATTCTGGAATTTGACTACAGCAGGCTCCCCAGGCAGAG CATCGGGCAGTTCCACGAGGGGGACGCCTATGTGGTCAAGTGGAAGTACATGGCAAGTACAGCAGTGGGA AGCCGACAAAAGGGAGAGCACCTGGTGAGAGTGGCTGGCAAAGAGAAGTGTGTCTATTTCTTCTGGCAAG GCCGGCACTCGACCGTGAGTGAGAAGGGAACGTCAGCTCTCATGACAGTAGAACTGGATGAAGAGAGGGG GGCCCAGGTCCAGGTCCTGCAGGGAAAGGAGCCACCCTGCTTCCTTCAGTGTTTCCAAGGGGGTATGGTG GTGCACTCCGGACGGAGGGAAGAGGAAGAAGAAAATGTGCAAAGTGAATGGAGACTGTATTGTGTGCGAG GAGAGGTGCCCATGGAAGGGAACTTGCTAGAAGTGGCCTGTCATTGCAGCAGTCTGAGGTCCAGGACTTC CATGGTCGTTCTGAATATAAACAAGGCCCTCATCTACTTGTGGCATGGATGCAAAGCTCAAGGTCATACG AAGGAGGTTGGAAGGACTGCAGCAAATAAGATCAAGGAAGAATGTCCCCTGGAAGCAGGTTTGCACAGCA GCAGCAACGTGACAATACATGAGTGTGACGAAGGATCCGAGCCCCTGGGATTCTGGGACGCTCTGGGGAG GAGGGACAGAAAGGCCTATGACTGCATGCTTCAAGATCCTGGAAGTTTTAACTTCGCGCCCCGCCTGTTC ATCCTCAGCAGCTCCTCCGGAGACTTCTCTGCGACAGAGTTTGTGTACCCCGCACAAGCGCCCTCTGCCG TCAGCTCCATGCCTTTCCTGCAGGAGGACCTGTACAGCGCGCCGCAGCCAGCTCTCTTCCTTGTTGACAA CCATCACGAGGTGTACCTCTGGCAAGGCTGGTGGCCCACTGAAAACAAGATAACCGGCTCTGCCCGCATT CGCTGGGCCTCAGACCGGAAGAGTGCCATGGAGACGGTCCTGCAGTACTGCCGAGGAAAGAATCTCAAAA GGCCACCCCCCAAGTCTTACCTTATTCATGCTGGGCTAGAGCCCCTGACATTCACCAACATGTTTCCCAG CTGGGAACACAGAGAAGACATTGCTGAAATCACAGAAATGGATACTGAAGTTTCCAACCAGATCACCTTG GTGGAAGATGTATTAGCCAAGCTCTGTAAAACCATCTATCCACTGGCAGATCTGCTTGCCAGGCCACTCC CAGAGGGGGTCGACCCTCTAAAGCTTGAGATTTATCTCACAGATGAAGACTTTGAGTTTGCACTCGACAT GTCCAGAGATGAATTCAATGCACTGCCCACCTGGAAGCAAGTGAACCTGAAGAAGTCAAAGGGCCTATTC TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_153153
Insert Size	6513 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_153153.3, NP_694793.1
RefSeq Size	7424 bp
RefSeq ORF	6513 bp
Locus ID	225115
UniProt ID	Q8K4L3
Gene Summary	Isoform 1: Forms a high-affinity link between the actin cytoskeleton and the membrane. Is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (a).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.