Shroom3 (NM_015756) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AL022960; D5Ertd287e; Shrm; Shrm3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225099 representing NM_015756 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACTCCGGAGAACTTGGAGGAGCCTAGCGCCACGCCAAACCCCAGTAGGACCCCCACGGAGAGAT TCGTTTATCTGGAAGCGCTCCTAGAGGGAGGGGCTCCCTGGGGCTTCACCCTGAAAGGTGGCCTGGAGCG TGGAGAACCGTTAATCATTTCCAAGATTGAAGAAGGGGGCAAAGCCGACTCAGTGAGCTCCGGACTGCAA GCTGGGGACGAAGTCATACACATCAATGAGGTGGCGCTGAGCAGTTCCAGGAGAGAGGCTGTCTCCCTAG TGAAAGGGTCCTACAAGACCCTGCGGCTGGTGGTGCGCAGAGACGTGTGTGCGGCCCCGGGCCATGCTGA TCCTGGTACCTCTAAGTCCCTCAGCTCAGAGCTGCTCACCTGCAGTCCCCAGCACCGGAAAGCAACATGG TCAGGAGGGGTGAAGCTTCGGCTAAAGCAGAGGTGCAGTGAGCCTGCCACTCGCCCTCACTCGTGGCATA CCACCAAATTTGGGGAGACACAGCCTGATGTCAGCATGATGCAGATATCTCAGGGCACAATGGGCCCTCC TTGGCACCAGAGCTACCATTCCAGCTCTTCGACCAGTGACCTCTCCAACTACGACCATGCTTATCTGAGG CGGAGCCCTGACCAGTGCAGCTCCCAGGGGAGCATGGAAAGCCTGGAGCCCAGTGGCGGATACCCACCCT GCCATCTCCTTTCTCCTGCCAAGTCCACGAGCAGCATCGACCAGCTAGGCCACCTTCATAACAAAAGGGA CTCCGCATATAGCTCCTTCTCCACCAGTTCGAGCATCTTCGAGTACCCACCCCCTGGTGGCTCTGCCCGA GAACGTTCGGGTTCCATGGACGTGATTTCTGCCCGGGGTGGCCTCCTAGAAGGGATGAGGCAGGCAGACA TCCGCTACGTCAAGACGGTCTATGATACCCGGAGAGGAGTATCTTCAGAGTATGAGGTGAACCCTTCAGC CTTGCTCCTTCAAGGTAGGGATGCCCATGCCTCAGCCGACAGTCAGGGCTGTGCTAAATGGCACAGCATT CCTCGGGGCAAGGGAACGCCTTCCCCGTCTTGGAGCCAGCAGTGTTCTGGTTCCCTGGAGACTGCTACTG ACAACCTGCCCCAGAAGGCAGGCGCACCCCTGCCCCCCACCCGCAGTGATAGCTATGCAGCCTTCCGCCA TCGCGAGCGTCCCAGCTCCTGGTCCAGCCTTGATCAGAAAAGGTTCTGCCGGCCTCAGACAAACTCTTCA GGCTCCCAGAAAACCCCATTCGCTGAGGACCAGCTGCATACAGTCCCAGAGAGGAGTCCAGAGAACAGCC CCCCAGTGAAATCTAAGCATAATTATACCCAGAAGGCGCAGCCTGGCCAACCTCTGCTGCCCACTGGCAT CTATCCGGTACCTTCTCCGGAGCCTCACTTTGCCCAGGTGCCCCAGCCCTCTGTGAGTAGTAATGGCACC GTCTACCCTGCGCTAGTCAAAGAGAGTGGATACACAGCTGCTCAGGGAACCTGTAACAAGATGGCTACCT TAGATGAGAACGGGAATCAAAATGAAGCTAGCAGGCCTGGGTTTGCCTTCTGTCAGCCCCTAGAACACAA CTCGGTGACTCCGGTGGAGAAAAGGCCAGAACCCACGGCCAAATACATCTACAAAGTCCATTTTTCTTCA GTGCCTGAAAATGAAGACTCCTCCCTAAAGAGGCACATCACACCTCCCCACGGCCATAGTCCATATCCCA GCGAGAGAAAGAACATCCACGGTGGCAGCAGAGCGTGTTCCAATCACCACAGTCTCTCGTCCCCCCAGGC CCAGGCCCTGCACGTGGGTGATGACAGGAGGCCTTCCAGGCTCTCTCAGCCCTGGGAGGGTGACTTTCAG GAAGATCACAATGCCAACCTCAGGCAGAAAGTGGAGAGGGAGGGCCAAGGCCAGGGCCTGTCGGGCAACT CTGGCAGGACCAGATCGGCCTTCTCATCTCTGCAGAATATTCCAGAGAGTCTGAGAAGACAAAGCAACGT GGAGCTTGGAGAAGCCCAGGAGGTTCACCCCGGGGGCAGGTCCAAGGTGGAGGACCCTGGAAGGAAAGCA GGGGCTTCAGACATCAGGGGCTACCTGGACAGATCTGTTTCCTATCCGAGGCCCGAGGGGAAAATGAACG CCGTAGACTCCGTCCACAGTGCAGACTCCAGGTATGAGGAGTCCCCGGCCCCAGCGCTGCCACAGACATC CGGTGCGAGCCAGCGGCGGCTCAGCTCCAGCAGCTCCGCCGCCCCGCAGTACAGGAAGCCCCATTGCTCC GTGCTGGAGAAGGTTTCTAGAATAGAGGAACGAGAGCAGGGGCGCCACAGACCCCTGAGCGTGGGCAGCT CTGCATACGGCCCCGGCTACAGGCCTGGGAGGACCGGCCCGACCCCCAGTACTTCCAGCAGTGACCTCGA CGATCCAAAAGCCGGCAGCGTCCATTTCTCGGAGTCCACTGAACACCTGCGCAATGGGGAGCAGAACCCC CCAAACGGGGAGGCTAAGCAGGAAGAGGCTTCCCGGCCCCAGTGCAGCCATTTGATCCGGAGGGCCCCTG CGGACGGCCGTGGACCTCCTGCCCGAGGCGGCGAGCCCTCAAGGCCGGAGGCCCGCCTGCTCCGCAGCCA GAGCACCTTTCAGCTCTACAGTGAGGCGGAGAGGGAAGCCTCGTGGTCAGAGGACAGGCCCGGTACCCCT GAGTCGCCCCTGCTCGATGCGCCTTTCAGCCGCGCCTACAGAAACAGCATCAAGGATGCCCAGTCCCGCG TCCTGGGGGCCACGTCGTTTCGTCGCCGAGACCTGGAACCGGGCACCCCCGCCACCTCGAGGCCCTGGCG CCCGCGACCCGCCTCCGCCCACGTGGGGATGCGGAGCCCCGAGGCCGCGGTGCCCTCGTCCTCCCCGCAC ACCCCTCGGGAGCGACACAGCGTGACCCCGGCCGCGCCCCAGGCTGCCCGCCGAGGCCCACGCCGGCGCC TCACTGTCGAGCAGAAGAAGCGCTCCTACTCGGAGCCCGAGAAGATGAACGAGGTAGGGGTCTCGGAGGA GGCCGAGCCCACGCCCTGCGGCCCGCCGAGGCCCGCGCAGCCGCGCTTCTCGGAGAGCACCGTGGCCGAC CGGCGCCGCATCTTCGAGCGCGACGGCAAGGCCTGCTCCACGCTCAGCCTGTCGGGCCCCGAGCTCAAGC AGTTCCAGCAGAGCGCCCTGGCCGACTACATCCAGCGTAAGACGGGCAAGCGGCCCACAGGAGCCGCCTG CACACCGGAGGCCGGGCTGCGCGAGCGCGCACAGAGCGCCTACCTCCAGGCCGGCCCCGCGGCGCCCGAT GGCCCCGGCCTGGCCTCTGCCTGTAGCCTGAGCTCCCTGCGAGAGCCCGAGGCGCTGCCCCGCAAGGAGC ACACTCATCCGTCGGCCGCGGACGGCCCGCAGGCGCCCCGTGATCGCAGCAGCTCCTTCGCCAGCGGACG CCTAGTCGGGGAGCGACGTCGCTGGGACCCTCAGGTCCCCAGGCAGCTGCTCAGCGGAGCGAACTGCGAA CCGAGGGGCGTCCAGAGGATGGACGGGGCCCCGGGCGGGCCGCCTTCCTGGGGCATGGTGGCCGGGAAGG CCGGGAAGTCCAAGTCAGCCGAGGATCTGCTGGAGCGCTCCGACACACTGGCGGTCCCTGTGCATGTGAG GTCCAGGTCGTCTCCCACCTCCGACAAGAAAGGTCAGGATGTGCTCCTGAGGGAAGGCAGCAACTTTGGT TTTGTAAAGGATCCATGCTGTTTGGCTGGCCCTGGACCTAGGTCTCTCAGCTGCTCAGACAAAGGCCAGA ATGAGCTGGCATTGCCCCTGCACCACCCTACGCCCTGCTGGAACGGCTCAGGCTGCAAAGCCACTGTGGC TTCCTCAGCTCCTCCCGAATCTTCTGGAGCCGCGGATCATCTGAAGCAACGTAGAGCGCCTGGTCCTAGG CCACTCTCAGCAGGAATGCACGGCCACTTCCCAGACGCCAGAGCTGCCTCACTCAGCTCCCCTCTGCCAT CACCTGTCCCCTCCAGTTACCGGTCACAGCTTGCCATGGATCAGCAGACTGGGCAACAGCCTCCCTCATC CCCTGCCTCTGCAGTGACCCAGCCTACCAGTCCAAGAAGCCCTGAGCTCAGCAGCCCAGCATATGGGCTA GGGGAGGGGATGTGGAAGAGGACATCCCTGCCTCAGCGACCCCCTCCCCCTTGGGTGAAATGGGCCCACG CGGTCAGAGAGGACGGCCTTGCTGAGGACACCTTGGCGCCTGAGTTTGCGAACCTGAAGCACTATAGAAA CCAGCCGAGTCGTCCGAGTTCCTGCAGCACTTCTGACCCAGACACTCCAGGAAGGATCTCGCTCCGAATA TCGGAGTCAGCCCTTCAACCTTCCCCACCGCCCCGGGGGGACTATGATGACGAAGTGTTCATGAAGGACT TGCACCCCAAGGTTACTTCAAGCCCCACATTTGAAGCTCTTCCCCCACCCCCGCCACCTTCGCCACCGAG CGAGGAACCTCTAGTGAATGGCACAGATGACTTTCCTCCGCCTCCTCCTCCCCAAGCCCTGTGTGAGGTA CTGCTGGATGGTGAGGCATCCACGGAAGCTGGCAGCGGCCCCTGCAGAATTCCCAGGGTGATGGTCACAA GGGAAGGGCACGTGCCTGGGGCAGCCCATTCCGAAGGTAGTCAGATAATGACTGCCACACCACCCCAAAC CTCGGCCAAAGGTTCAGAAGCCGAGTCCAACACGCCTTCCAGCGCGAGCGCCCAGCCTCAGCTCAACGGT TCTCCTGGCAAGCAGCTCTGTCCCAGCCAGACCCGAAACCTCACTTATGAACCTGTCGAGAGAACTCAGG ATCTAGGAAAGAAAACTCACGCCGAGCCTCAGAAGACCTCAGAAGATATCCGGACAGAGGCTCTAGCCAA GGAAATTGTCCACCAAGACAAGTCTCTGGCAGACATTTTAGATCCTGACTCCAGGATGAAGACAACGATG GACCTGATGGAGGGTTTATTCCCTGGAGACGCCAGTGTGCTGATGGATAGTGGCGCAAAGAGGAAAGCCT TAGACATCACCGCCAGGCGTGCGGGATGTGAAGCCAAGGCGAGCGACCACAAGGAGGCAGTGAGTGTGCT GGTCAACTGTCCTGCCTACTACAGTGTCTCTGCTGCCAAGGCGGAGCTGCTCAACAAAATCAAAGACATG CCGGAGGAGCTGCAGGAGGAGGAGGGGCAGGAGGATGTCAATGAGAAAAAGGCTGAGCTTATCGGAAGCC TTACCCACAAGCTGGAGAGCCTCCAGGAAGCCAAGGGGAGCCTGCTCACGGACATCAAGCTGAACAATGC CCTGGGAGAGGAGGTGGAGGCTCTGATCAGTGAGCTCTGCAAACCCAACGAGTTTGACAAGTACAAGATG TTCATAGGGGACCTGGACAAGGTGGTCAACCTGCTGCTGTCCCTGTCTGGACGCCTGGCCCGCGTGGAGA ACGTCCTCAGAGGCCTTGGTGAAGACGCCAGCAAAGAGGAAAGGAGTTCTCTGAACGAGAAAAGGAAGGT TCTGGCTGGCCAGCATGAGGATGCACGGGAGCTCAAGGAGAACCTGGACCGGAGGGAGCGCGTGGTGCTG GATATCCTGGCCAACTACCTGTCCGCCGAGCAGCTGCAGGACTACCAGCACTTCGTGAAGATGAAGTCCA CACTCCTCATTGAGCAGCGGAAGCTGGATGACAAGATAAAGCTGGGCCAGGAGCAGGTCAGGTGCCTGCT GGAGTCGCTTCCCTCGGACTTCAGGCCCAAGGCAGGGGCCATCTCCCTCCCTCCAGCCCTCACCGGCCAT GGGACTCCTGGGGGGACATCTGTATTCGGTGGTGTTTTCCCAACGTTGACCTCTCCTCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_015756
Insert Size	5943 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015756.2, NP_056571.2
RefSeq Size	7210 bp
RefSeq ORF	5943 bp
Locus ID	27428
UniProt ID	Q9QXN0
Gene Summary	Controls cell shape changes in the neuroepithelium during neural tube closure. Induces apical constriction in epithelial cells by promoting the apical accumulation of F-actin and myosin II, and probably by bundling stress fibers. Induces apicobasal cell elongation by redistributing gamma-tubulin and directing the assembly of robust apicobasal microtubule arrays.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).

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