Lamb1 (NM_008482) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C77966; C80098; C81607; D130003D08Rik; Lamb-1; Lamb1-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224961 representing NM_008482 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGGCCCCTCTCCTCTCTCCCAACATTTGCCTTTTCTCCCCGCTACCTCTCCAGAAAGGAAGACC CGAAGAAAAGACAGGCAGCTTGCCTGCTGCGTCCTCCTTCCCGTGCCGCGTCCCCTCGTCTGCGAGGACT GGACATGGGGCTGCTCCAGGTGTTCGCCTTTGGTGTCCTAGCCCTATGGGGCACCCGAGTGTGCGCTCAG GAACCGGAGTTCAGCTATGGCTGCGCAGAAGGCAGCTGCTACCCTGCCACTGGCGACCTTCTCATCGGCC GAGCGCAAAAGCTCTCCGTGACTTCGACATGTGGACTGCACAAACCAGAGCCCTACTGTATTGTTAGCCA CCTGCAGGAGGACAAGAAATGCTTCATATGTGACTCCCGAGACCCTTATCACGAGACCCTCAACCCCGAC AGCCATCTCATTGAGAACGTGGTCACCACATTTGCTCCAAACCGCCTTAAGATCTGGTGGCAATCGGAAA ATGGTGTGGAGAACGTGACCATCCAACTGGACCTGGAAGCAGAATTCCATTTCACTCATCTCATCATGAC CTTCAAGACATTCCGCCCAGCCGCCATGCTGATCGAGCGGTCTTCTGACTTTGGGAAGGCTTGGGGCGTG TACAGATACTTCGCCTACGACTGTGAGAGCTCGTTCCCAGGCATTTCAACTGGACCCATGAAGAAAGTGG ATGACATCATCTGTGACTCTCGATATTCTGACATTGAGCCCTCGACAGAAGGAGAGGTAATATTTCGTGC TTTAGATCCTGCTTTCAAAATTGAAGACCCTTATAGTCCAAGGATACAGAATCTATTAAAAATCACCAAC TTGAGAATCAAGTTTGTGAAACTGCACACCTTGGGGGATAACCTTTTGGACTCCAGAATGGAAATCCGAG AGAAGTACTATTACGCTGTTTATGATATGGTGGTTCGAGGGAACTGCTTCTGCTATGGCCACGCCAGTGA ATGCGCCCCTGTGGATGGAGTCAATGAAGAAGTGGAAGGAATGGTTCACGGGCACTGCATGTGCAGACAC AACACCAAAGGCCTGAACTGTGAGCTGTGCATGGATTTCTACCACGATTTGCCGTGGAGACCTGCTGAAG GCCGGAACAGCAACGCCTGCAAAAAATGTAACTGCAATGAACATTCCAGCTCGTGTCACTTTGACATGGC AGTCTTCCTGGCTACTGGCAACGTCAGCGGGGGAGTGTGTGATAACTGTCAGCACAACACCATGGGGCGC AACTGTGAACAGTGCAAACCGTTCTACTTCCAGCACCCTGAGAGGGACATCCGGGACCCCAATCTCTGTG AACCATGTACCTGTGACCCAGCTGGTTCTGAGAATGGCGGGATCTGTGATGGGTACACTGATTTTTCTGT GGGTCTCATTGCTGGTCAGTGTCGGTGCAAATTGCACGTGGAGGGAGAGCGCTGTGATGTTTGTAAAGAA GGCTTCTACGACTTAAGTGCTGAAGACCCGTATGGTTGTAAATCATGTGCTTGCAATCCTCTGGGAACAA TTCCTGGTGGGAATCCTTGTGATTCTGAGACTGGCTACTGCTACTGTAAGCGCCTGGTGACAGGACAGCG CTGTGACCAGTGCCTGCCGCAGCACTGGGGTTTAAGCAATGATTTGGATGGGTGTCGACCTTGTGACTGT GACCTTGGAGGGGCGCTGAACAATAGCTGCTCCGAGGACTCCGGCCAGTGCTCCTGCCTGCCCCACATGA TTGGGCGGCAGTGTAACGAGGTGGAGTCCGGTTACTACTTCACCACCCTGGACCACTACATCTACGAAGC CGAGGAAGCCAATCTGGGGCCTGGAGTCATTGTGGTGGAAAGGCAGTACATTCAGGACCGCATTCCTTCC TGGACAGGACCTGGGTTCGTCCGGGTGCCTGAAGGGGCTTATTTGGAGTTTTTCATTGACAACATACCGT ATTCCATGGAGTATGAAATCCTGATTCGCTATGAGCCACAGCTGCCGGACCACTGGGAGAAAGCTGTCAT CACTGTACAGCGGCCGGGGAAGATTCCAGCCAGCAGCCGATGTGGTAACACCGTTCCCGATGATGACAAC CAGGTGGTGTCCTTGTCACCGGGCTCAAGATACGTTGTCCTCCCTCGCCCCGTGTGCTTTGAGAAGGGAA TGAACTACACGGTGAGGTTGGAGCTGCCCCAGTATACGGCATCGGGCAGTGACGTGGAGAGCCCTTACAC GTTCATCGACTCGCTTGTTCTCATGCCCTACTGTAAATCGCTGGACATCTTCACTGTTGGCGGCTCAGGC GATGGGGAGGTCACCAATAGTGCCTGGGAAACCTTCCAGCGCTACAGGTGTCTGGAGAACAGCAGGAGTG TGGTAAAAACACCCATGACAGATGTCTGCAGAAACATTATCTTCAGCATTTCTGCCTTGATTCACCAGAC GGGCCTTGCTTGTGAATGTGACCCCCAGGGATCTCTGAGTTCTGTGTGTGACCCCAATGGTGGCCAGTGC CAGTGCCGTCCTAATGTGGTTGGAAGAACCTGCAACAGGTGTGCCCCGGGCACCTTTGGCTTTGGCCCCA ACGGATGCAAACCTTGTGACTGCCATCTGCAAGGGTCTGCCAGTGCCTTCTGCGATGCGATCACTGGCCA GTGCCACTGTTTCCAGGGCATCTATGCTCGGCAGTGTGACCGATGTCTCCCTGGGTATTGGGGCTTTCCC AGCTGCCAGCCCTGCCAGTGTAATGGTCATGCTCTAGACTGTGACACAGTGACAGGGGAGTGTCTGAGCT GTCAGGACTACACCACGGGCCACAACTGCGAAAGGTGCCTGGCTGGCTACTACGGTGATCCCATCATTGG GTCAGGAGACCACTGTCGCCCTTGCCCTTGTCCTGATGGTCCTGACAGTGGACGACAGTTTGCCAGGAGC TGTTATCAAGACCCCGTCACTCTCCAGCTTGCGTGTGTTTGTGATCCTGGGTACATTGGCTCCAGATGTG ATGACTGTGCCTCTGGATTTTTTGGCAATCCCTCAGACTTTGGGGGTTCATGTCAACCATGTCAGTGCCA CCACAACATTGACACTACCGATCCAGAAGCCTGTGACAAGGAGACGGGACGATGCCTCAAGTGCCTGTAC CACACAGAAGGGGACCATTGCCAGCTCTGCCAGTATGGGTACTACGGCGATGCTCTTCGGCAAGACTGTA GAAAGTGTGTCTGCAATTACCTGGGCACGGTGAAGGAACATTGTAATGGCTCTGACTGCCACTGTGACAA AGCCACTGGTCAGTGCTCGTGCCTTCCCAATGTGATCGGGCAGAACTGTGACCGGTGTGCGCCCAACACC TGGCAGCTGGCTAGCGGGACTGGCTGCGGGCCCTGCAATTGCAATGCTGCGCATTCCTTTGGGCCATCCT GCAACGAGTTCACAGGGCAGTGCCAGTGCATGCCGGGCTTTGGAGGCCGAACCTGCAGCGAGTGCCAGGA GCTCTTCTGGGGAGACCCTGATGTGGAATGCCGAGCCTGTGACTGTGATCCCAGGGGCATTGAGACACCT CAGTGTGACCAGTCCACGGGCCAGTGTGTCTGTGTGGAGGGTGTAGAGGGTCCTCGCTGCGACAAGTGCA CCAGAGGTTACTCGGGGGTCTTTCCTGACTGCACACCCTGCCACCAGTGCTTTGCTCTCTGGGATGCTAT CATTGGTGAGCTGACCAACAGGACCCACAAATTCCTGGAGAAAGCCAAGGCTCTGAAAATCAGTGGTGTG ATTGGTCCCTACCGAGAGACCGTGGACTCTGTAGAGAAGAAAGTCAATGAGATAAAAGACATCCTGGCCC AGAGCCCAGCAGCGGAACCACTGAAAAACATTGGCATTCTCTTCGAGGAGGCAGAGAAACTAACCAAAGA TGTCACAGAAAAGATGGCGCAGGTAGAAGTGAAATTAACTGATACAGCTTCACAGAGTAACAGCACAGCT GGAGAGCTCGGCGCACTGCAGGCAGAAGCAGAGAGCCTTGACAAGACCGTGAAGGAGCTGGCAGAACAGC TGGAGTTTATCAAAAACTCCGATATTCAGGGCGCCTTGGATAGCATCACCAAGTATTTCCAGATGTCTCT TGAGGCAGAGAAGCGGGTGAATGCCTCCACCACAGACCCCAACAGCACTGTGGAGCAGTCTGCCCTCACG CGAGACAGAGTAGAAGATCTGATGTTGGAGCGAGAGTCTCCGTTCAAGGAGCAGCAGGAGGAACAGGCAC GCCTCCTGGACGAACTGGCCGGCAAACTGCAAAGTCTCGACCTGTCGGCTGTTGCACAGATGACCTGTGG AACACCTCCAGGGGCTGACTGTTCTGAAAGTGAATGTGGTGGCCCCAACTGCAGAACTGACGAAGGAGAG AAGAAGTGTGGGGGGCCTGGCTGTGGTGGTCTGGTCACTGTGGCCCACAGTGCTTGGCAGAAAGCCATGG ATTTTGACCGTGATGTCCTGAGTGCCCTGGCTGAAGTCGAACAGCTCTCCAAGATGGTCTCTGAAGCAAA AGTGAGAGCAGATGAGGCGAAGCAGAATGCGCAGGATGTCCTGTTAAAAACAAATGCTACCAAAGAAAAA GTGGACAAGAGCAACGAGGACCTGCGGAACCTCATCAAGCAGATCAGAAACTTCCTGACTGAGGATAGTG CTGATCTAGACAGTATTGAAGCAGTTGCTAATGAAGTACTGAAAATGGAAATGCCTAGCACGCCACAGCA GTTACAGAACCTAACAGAAGACATTCGGGAGCGAGTTGAAACCCTCTCTCAAGTAGAGGTTATTTTGCAG CAGAGTGCAGCTGACATTGCCAGAGCTGAGCTGTTGCTTGAGGAAGCTAAGAGAGCAAGCAAAAGTGCAA CAGATGTTAAAGTCACTGCAGACATGGTGAAGGAAGCATTAGAAGAAGCAGAAAAGGCCCAGGTTGCAGC AGAGAAGGCGATTAAACAAGCTGATGAGGATATCCAAGGAACCCAAAACCTGCTAACATCGATTGAATCT GAAACGGCAGCTTCTGAGGAAACCCTGACCAACGCCTCCCAGCGCATCAGCAAGCTTGAGAGGAACGTGG AAGAGCTTAAGCGTAAAGCTGCCCAGAACTCTGGGGAGGCAGAATATATCGAAAAAGTAGTATATTCTGT AAAACAGAATGCAGACGATGTTAAGAAGACTCTAGATGGCGAACTTGATGAAAAGTATAAGAAGGTAGAA AGTTTAATTGCCCAAAAAACTGAAGAGTCAGCAGATGCCAGGAGGAAAGCTGAGCTGCTACAAAATGAAG CAAAAACACTCTTGGCTCAAGCTAACAGCAAGCTCCAGCTGTTGGAAGACTTAGAAAGAAAATATGAGGA CAATCAAAAATACTTAGAAGATAAAGCTCAAGAATTGGTGCGACTGGAAGGAGAGGTTCGCTCCCTCCTT AAGGACATAAGTGAGAAAGTTGCGGTTTACAGCACCTGCTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_008482
Insert Size	5505 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_008482.2, NP_032508.2
RefSeq Size	5778 bp
RefSeq ORF	5505 bp
Locus ID	16777
Gene Summary	Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of the cerebral cortex (By similarity). It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons (By similarity). Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface (By similarity).[UniProtKB/Swiss-Prot Function]

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