Rbbp6 (NM_011247) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933422O15Rik; AI316869; BB233631; C030034J04Rik; C77662; P2P-R; PACT
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224931 representing NM_011247 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTGTGTGCACTATAAATTTTCCTCTAAACTCAACTACGACACCGTCACTTTTGATGGGCTCCATA TCTCCCTCTGCGATTTAAAGAAGCAGATTATGGGGAGAGAAAAGCTGAAAGCTGCCGATAGCGATCTGCA GATCACCAACGCACAGACGAAAGAAGAATACACTGATGACAATGCGCTCATTCCTAAGAACTCATCTGTG ATTGTCAGGAGGATTCCTATTGGAGGTGTCAAGTCTACAAGCAAGACATATGTTATAAGTCGAACTGAAC CAGTGATGGGAACTACAAAAGCAATTGATGACGCTTCTGCATCTATTTCTCTGGCCCAGCTTACAAAGAC TGCCAATCTGGCTGAAGCCAATGCTTCTGAAGAAGACAAAATTAAAGCAATGATGTCACAGTCTGGCCAT GAATACGACCCAATCAATTACATGAAGAAAACTCTAGTAGGTCCACCACCTCCATCTTACACCTGCTTTC GTTGTGGTAAACCTGGTCATTATATTAAGAATTGCCCAACAAATGGGGATAAGAACTTTGAATCTGGTCC TAGGATCAAAAAGAGCACTGGAATTCCTAGAAGTTTTATGATGGAAGTGAAAGATCCTAACATGAAAGGT GCAATGCTTACAAACACTGGAAAATATGCAATACCAACTATAGATGCAGAGGCCTATGCAATCGGGAAGA AAGAGAAACCACCCTTCTTACCAGAGGAGCCATCATCATCTTCAGAAGAAGATGATCCTATCCCAGACGA GCTCTTGTGCCTCATCTGCAAAGACATCATGACTGATGCTGTGGTCATTCCCTGCTGTGGAAACAGTTAC TGTGATGAATGTATAAGAACAGCACTCTTGGAGTCAGATGAACATACATGTCCAACATGTCACCAAAATG ATGTTTCTCCTGATGCTTTAATTGCCAACAAGTTTTTACGACAGGCTGTTAATAACTTTAAAAATGAAAC TGGCTATACAAAACGACTACGAAAACAGTTACCTCCACCCCCACCCCCAGTACCACCACCAAGACCACTC ATGCAGCGGAACCTGCAGCCTCTGATGAGATCTCCCATATCAAGACAGCAGGATCCTCTGATGATTCCAG TGACATCGTCCTCCGCTCACTCAGCTCCCTCTATATCTTCATTAACTTCAAATCCATCTGCCCTGGCTCC TTCTGTGTCTGGAAATCCATCTTCTGCCCCAGCTCCAGTACCTGATATAACTGCAACCGTGTCTATATCA GTCCACTCAGAAAAATCGGATGGACCTTTTCGGGATTCTGATAATAAATTATTGCCAGCTGCCGCCCTTA CATCAGAACATTCAAAGGGAGCCTCTTCAATTGCTATTACTGCTCTTATGGAAGAAAAGGGGTACCAGGT ACCAGTCCTTGGAACTCCATCTTTGTTGGGACAGTCATTATTACATGGACAGTTGATTCCCACAACTGGC CCAGTAAGAATCAATGCTGCTCGTCCAGGTGGTGGCCGGCCAGGCTGGGAGCATTCCAACAAGCTTGGGT ACCTAGTTTCTCCACCACAGCAAATTAGAAGAGGAGAAAGAAGCTGTTACAGAAGTATAAACCGCGGGCG ACACCACAGCGAACGATCACAGAGGACTCAAGGCCCATCACTTCCAGCAACTCCAGTCTTTGTGCCCGTT CCACCACCTCCTTTGTATCCGCCTCCTCCCCATACACTTCCTCTTCCTCCAGGTGTACCTCCCCCACAGT TTTCTCCTCAGTTTCCTCCTGGCCAGCCTCCACCAGCAGGATATAGTGTCCCTCCTCCAGGGTTTCCACC AGCTCCTGCCAATATATCAACACCTTGGGTATCATCAGGAGTGCAGACTGCCCATTCAAATACCATCCCT ACAACACAAGCACCTCCTTTGTCCAGGGAAGAATTCTATAGAGAGCAACGACGGCTAAAGGAAGAGGAAA AGAAAAAGTCCAAGCTAGATGAGTTTACAAATGATTTTGCTAAGGAATTGATGGAATACAAAAAGATTCA AAAGGAGCGTAGGCGCTCATTTTCCAGGTCTAAATCTCCCTATAGTGGGTCATCGTATTCAAGAAGTTCA TACACTTATTCAAAGTCAAGGTCTGGCTCAACACGTTCACGCTCTTACTCTCGGTCCTTCAGCCGCTCAC ATTCTCGCTCCTATTCACGATCACCCCCATACCCCAGGAGAGGCAGAGGCAAGAGCCGCAATTACCGTTC ACGGTCCAGATCTCACGGATACCACCGATCTAGGTCAAGGTCACCTCCCTATAGACGATACCACTCACGG TCCAGATCTCCTCAAGCATTTAGGGGACAGTCTCCCACTAAACGCAATGTACCTCAAGGAGAAACAGAGC GTGAGTATTTTAATAGATACAGAGAAGTTCCACCCCCTTATGACATCAAAGCCTATTATGGGCGGAGTGT CGACTTTAGAGACCCATTTGAGAAAGAACGCTACCGGGAATGGGAAAGGAAATACCGAGAGTGGTATGAG AAGTACTACAAAGGGTACGCGGTGGGAGCTCAACCTAGACCCTCAGCCAATAGAGAGGACTTTTCTCCAG AGAGACTCTTACCTCTTAATATCAGAAATTCACCCTTTACAAGAGGGCGCAGAGAAGACTATGCTGCTGG ACAAAGTCATAGAAATAGAAATCTAGGTGGCAACTATCCAGAAAAGCTTTCAACAAGGGACAGTCACAAT GCAAAAGATAATCCAAAATCGAAGGAGAAGGAGAGTGAGAATGTTCCAGGAGACGGCAAAGGGAACAAGC ATAAGAAACACAGGAAACGAAGAAAGGGGGAAGAGAGTGAGAGCTTCCTGAACCCAGAGCTACTGGAGAC GTCTAGGAAATGCAGGGAATCGTCAGGGATTGATGAAACGAAGACAGATACACTGTTTGTTCTCCCAAGC AGAGACGATGCTACACCTGTTAGGGATGAGCCAATGGACGCAGAATCGATCACTTTCAAGTCAGTATCTG ACAAAGACAAGAGGGAAAAGGATAAGCCAAAAGTAAAAAGTGACAAGACCAAACGGAAAAGTGACGGGTC TGCTACAGCCAAGAAAGACAATGTTTTAAAACCTTCTAAAGGACCTCAAGAAAAGGTAGATGGAGACCGT GAAAAGTCTCCTCGGTCTGAGCCGCCACTCAAAAAAGCCAAAGAGGAGGCTACAAAGATTGACTCTGTAA AACCTTCCTCGTCTTCTCAGAAGGATGAGAAGGTCACTGGAACCCCTAGAAAAGCCCATTCTAAATCTGC AAAAGAACACCAGGAGGCAAAGCCAGCCAAGGACGAGAAGGTCAAAAAGGACTGTTCCAAAGACATCAAG TCAGAAAAGCCAGCCAGTAAGGACGAGAAGGCCAAGAAGCCTGAGAAAAATAAACTACTTGATAGCAAGG GAGAAAAACGAAAGAGAAAAACGGAAGAAAAGAGTGTAGATAAAGATTTTGAGTCGTCTTCAATGAAAAT CTCTAAAGTAGAAGGAACAGAAATAGTGAAACCATCACCAAAACGGAAAATGGAAGGTGATGTTGAAAAG CTGGAAAGGACCCCAGAAAAGGACAAGATTGCATCATCAACTACTCCAGCCAAAAAAATCAAACTCAACA GAGAAACTGGAAAAAAAATTGGAAATGCAGAAAATGCATCTACTACAAAAGAACCCTCTGAAAAATTGGA GTCAACATCTAGCAAAATCAAACAGGAAAAAGTCAAGGGAAAGGCCAAACGGAAAGTAGCTGGGTCGGAA GGCTCCAGCTCCACGCTTGTGGATTACACCAGTACAAGTTCAACTGGAGGCAGTCCTGTGAGGAAATCTG AAGAAAAGACAGATACAAAGCGAACAGTCATTAAAACTATGGAGGAATATAATAATGATAACACAGCTCC TGCTGAAGATGTTATAATTATGATCCAGGTTCCTCAGTCCAAATGGGATAAAGATGACTTTGAGTCTGAA GAAGAAGATGTTAAAACCACACAACCTATACAGAGTGTAGGGAAACCATCGAGTATTATAAAAAATGTCA CTACTAAGCCATCGGCTACGGCTAAGTACACCGAGAAGGAAAGCGAGCAGCCCGAGAAACTGCAGAAGCT TCCCAAGGAGGCGAGCCACGAGCTGATGCAGCACGAGCTCAGGAGCTCAAAGGGCAGTGCGTCCAGTGAG AAGGGCAGAGCCAAGGACCGGGAGCACTCAGGGTCGGAGAAGGACAACCCTGACAAGAGGAAGAGCGGTG CCCAGCCAGACAAGGAGAGCACTGTGGACCGCCTGAGTGAGCAGGGACATTTTAAGACTCTCTCTCAGTC TTCCAAAGAGACCAGGACTTCAGAGAAGCACGAGTCTGTTCGTGGTTCCTCAAATAAAGACTTCACTCCT GGTAGAGACAAGAAAGTGGACTACGACAGCAGGGATTATTCCAGTTCCAAGCGAAGAGACGAGAGAGGTG AATTAGCAAGGAGAAAAGACTCTCCTCCCCGGGGCAAAGAGTCTCTGTCTGGGCAGAAAAGCAAGCTGAG GGAGGAGAGAGATTTACCTAAAAAGGGGGCCGAGTCAAAAAAAAGTAATTCTAGCCCCCCAAGAGACAAA AAGCCTCATGATCATAAAGCCCCCTACGAAACTAAACGCCCATGTGAAGAGACAAAGCCTGTAGATAAAA ACTCTGGGAAGGAGCGGGAGAAGCATGCTGCTGAAGCTCGCAATGGGAAAGAGTCCAGTGGTGGCAAACT GCCATGTATACCTAACCCGCCAGACCCTCCCATGGAGAAGGAGCTGGCTGCTGGGCAGGTGGAGAAGAGC GCCGTCAAGCCGAAACCCCAGCTGAGCCATTCCTCGAGGCTTTCCTCTGACCTGACCCGGGAGACGGACG AGGCTGCCTTTGAACCAGATTATAATGAGAGCGACAGTGAGAGTAATGTGTCTGTGAAGGAAGAAGAAGC TGTTGCCAGTATCTCCAAGGACTTGAAAGAGAAAACAACAGAGAAAGCGAAAGAGAGCTTGACTGTAGCA ACGGCCAGCCAGCCAGGTGCAGACAGGAGCCAGAGCCAAAGTAGCCCCAGTGTTAGTCCAAGTAGAAGTC ATAGCCCTTCCGGGAGCCAGACCCGAAGCCACAGCAGCAGTGCCAGCTCAGCCGGAAGCCAGGACAGCAA AAAGAAGAAGAAGAAGAAGGAGAAGAAAAAGCACAAGAAGCATAAAAAGCACAAGAAGCACAAGAAGCAC GCAGGCGCCGACGGCGACGTGGAGAAGAGCCAGAAACACAAACACAAGAAGAAGAAGGCCAAGAAGAACA AAGACAAGGAGAAGGAGAAAGATGACCAAAAAGTGAGATCTGTCACTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_011247
Insert Size	5373 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011247.2, NP_035377.2
RefSeq Size	6199 bp
RefSeq ORF	5373 bp
Locus ID	19647
UniProt ID	P97868
Gene Summary	E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome (By similarity). May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth retardation (PubMed:17470788). Regulates DNA-replication and common fragile sites (CFS) stability in a ZBTB38- and MCM10-dependent manner. Controls ZBTB38 protein stability and abundance via ubiquitination and proteasomal degradation, and ZBTB38 in turn negatively regulates the expression of MCM10 which plays an important role in DNA-replication (PubMed:24726359).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the full-length transcript and encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.