Col4a4 (NM_007735) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	E130010M05Rik; [a]4(IV)
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224813 representing NM_007735 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTGCTTTTTCAGATGGACCAAGTCCTTTGTCACAGCCCCCTGGTCACTAATATTTATCCTCTTTA CTATACAATATGAATATGGGAGTGGCAAGAAGTATGGCGGCCCCTGCGGAGGAAGAAACTGCTCTGTCTG CCAGTGTTTTCCTGAGAAGGGATCTCGGGGTCACCCAGGACCACTGGGACCACAGGGACCAATAGGGCCC TTGGGACCCCTGGGACCTATTGGGATTCCAGGAGAAAAAGGAGAGAGAGGAGACAGTGGCTCACCTGGAC CACCAGGAGAGAAAGGAGACAAGGGTCCAACTGGTGTCCCTGGATTTCCAGGTGTGGATGGTGTACCTGG TCACCCAGGGCCTCCTGGACCCAGAGGCAAACCTGGTGTGGACGGCTACAATGGCTCACGTGGTGATCCG GGCTATCCAGGAGAAAGAGGAGCTCCAGGCCCAGGCGGCCCCCCTGGTCAGCCTGGGGAAAATGGAGAAA AAGGAAGATCTGTGTACATTACTGGTGGCGTTAAAGGTATTCAGGGAGACCGTGGGGACCCAGGACCACC CGGCTTACCAGGATCTAGGGGTGCACAAGGGTCACCAGGGCCCATGGGGCATGCAGGCGCACCAGGGCTA GCAGGACCTATAGGTCATCCTGGAAGCCCTGGGTTGAAGGGAAATCCTGCCACTGGACTGAAGGGACAAA GGGGAGAGCCGGGTGAAGTTGGCCAGCGTGGTCCTCCTGGGCCCACGCTGTTGGTACAGCCGCCTGATTT GAGTATCTATAAAGGAGAAAAGGGTGTAAAAGGAATGCCTGGAATGATTGGTCCCCCAGGACCTCCAGGT CGCAAGGGAGCGCCTGGTGTCGGGATCAAAGGAGAGAAAGGTATCCCTGGGTTCCCAGGACCCCGGGGTG AGCCTGGTTCGCATGGGCCTCCAGGTTTTCCAGGATTCAAGGGGATACAAGGAGCAGCTGGAGAGCCTGG GCTGTTCGGATTTCTTGGTCCAAAGGGGGATCTGGGAGATCGCGGGTACCCGGGACCACCAGGCATTTTG TTGACTCCAGCTCCACCACTCAAAGGTGTTCCAGGGGACCCAGGACCCCCTGGCTACTATGGGGAGATTG GAGATGTTGGATTACCAGGTCCTCCAGGGCCCCCAGGCAGACCAGGGGAAACTTGTCCAGGCATGATGGG ACCTCCTGGACCACCAGGGGTTCCTGGTCCTCCAGGATTTCCAGGAGAAGCTGGGGTTCCTGGAAGACTC GACTGTGCCCCAGGAAAACCTGGGAAGCCAGGGCTACCCGGCCTGCCTGGCGCACCAGGACCAGAGGGGC CCCCTGGATCAGATGTGATATACTGTCGCCCTGGGTGCCCTGGGCCAATGGGTGAAAAGGGAAAAGTGGG TCCTCCAGGAAGAAGAGGAGCCAAAGGAGCAAAAGGAAACAAGGGACTCTGTACCTGTCCACCTGGTCCC ATGGGACCCCCTGGTCCCCCAGGACCTCCTGGAAGACAAGGAAGTAAAGGAGACTTAGGGCTCCCTGGTT GGCATGGAGAAAAGGGCGATCCAGGTCAGCCTGGTGCTGAAGGACCGCCAGGGCCACCAGGAAGACCTGG TGCCATGGGGCCCCCTGGTCACAAAGGGGAAAAGGGTGACATGGTCATATCAAGAGTGAAAGGGCAAAAA GGAGAAAGAGGACTGGATGGGCCACCAGGATTTCCGGGGCCACATGGACAAGATGGTGGGGATGGACGTC CTGGAGAAAGAGGGGATCCTGGGCCACGAGGGGACCATAAGGATGCAGCCCCGGGTGAGAGAGGGCTTCC CGGACTGCCAGGCCCTCCAGGAAGAACAGGACCTGAGGGGCCTCCAGGCCTGGGATTTCCAGGCCCACCA GGACAGCGAGGCCTACCCGGAGAGCCCGGGCGTCCGGGCACCAGAGGCTTTGATGGCACGAAGGGGCAGA AAGGTGACTCCATTCTGTGTAATGTAAGCTACCCGGGAAAACCAGGTCTGCCAGGTCTTGATGGACCTCC AGGTCTGAAGGGGTTTCCAGGCCCCCCAGGGGCTCCTGGTATGAGATGTCCAGATGGGCAGAAAGGCCAG CGGGGCAAACCGGGAATGTCTGGGATTCCAGGTCCACCTGGTTTTCGTGGTGACATGGGAGATCCAGGCA TCAAAGGTGAAAAGGGGACCTCCCCTATTGGACCCCCAGGCCCACCTGGGTCTCCTGGAAAGGATGGCCA GAAAGGAATCCCAGGAGACCCTGCATTTGGTGACCCAGGACCCCCTGGAGAGAGGGGTCTTCCAGGAGCA CCAGGCATGAAAGGACAGAAAGGTCACCCAGGGTGCCCAGGGGCTGGAGGCCCTCCTGGCATCCCTGGAT CTCCAGGTCTCAAAGGTCCCAAAGGTAGAGAGGGAAGTCGTGGCTTTCCAGGGATCCCAGGATCCCCTGG CCATTCCTGTGAAAGAGGTGCTCCCGGGATCCCAGGACAACCGGGACTTCCTGGAACTCCAGGTGATCCA GGGGCCCCAGGTTGGAAAGGCCAGCCAGGAGACATGGGTCCCTCTGGACCAGCTGGGATGAAGGGCCTCC CTGGTCTCCCAGGCCTACCAGGAGCTGATGGTCTCCGAGGGCCTCCTGGGATTCCAGGCCCCAATGGGGA AGATGGTCTACCTGGTCTTCCAGGCCTGAAGGGACTCCCAGGGCTGCCTGGCTTCCCTGGTTTTCCAGGA GAGAGAGGAAAGCCAGGGCCAGATGGAGAACCTGGAAGAAAGGGAGAGGTTGGAGAGAAAGGCTGGCCTG GCTTGAAGGGAGACCTGGGAGAGCGAGGCGCCAAAGGAGACAGAGGACTTCCTGGTGATGCAGGAGAAGC AGTCACTTCCAGAAAGGGTGAACCTGGGGATGCTGGGCCTCCAGGAGATGGTGGGTTCTCAGGAGAGAGA GGTGATAAAGGAAGCTCAGGGATGCGAGGGGGAAGAGGGGATCCCGGAAGAGATGGACTACCGGGACTCC ACAGAGGGCAGCCTGGGATAGATGGGCCTCCTGGGCCTCCTGGGCCCCCTGGCCCTCCAGGATCACCTGG ACTGAGAGGAGTCATTGGTTTCCCAGGATTTCCAGGTGACCAGGGTGATCCAGGTTCTCCAGGACCCCCT GGATTCCCAGGAGATGATGGAGCAAGAGGACCTAAAGGATACAAAGGTGACCCTGCCAGTCAGTGTGGTC CACCTGGTCCAAAGGGTGAGCCAGGTAGTCCTGGATACCAAGGACGTACTGGAGTCCCCGGAGAGAAAGG CTTTCCTGGAGATGAAGGACCCCGAGGACCACCAGGCAGACCTGGACAGCCTGGATCTTTTGGACCACCA GGGTGTCCAGGTGACCCAGGGATGCCCGGACTCAAGGGACATCCAGGAGAAGTGGGAGACCCTGGGCCAA GAGGTGATGCGGGAGATTTCGGGAGGCCAGGTCCTGCTGGAGTGAAAGGGCCTCTCGGGTCTCCTGGTCT GAATGGCTTACATGGTCTGAAGGGTGAGAAAGGAACAAAAGGCGCCTCAGGTTTGCTCGAAATGGGTCCA CCTGGGCCAATGGGGATGCCTGGGCAAAAAGGAGAGAAAGGAGACCCTGGGAGTCCAGGGATTTCTCCTC CAGGTCTTCCTGGAGAAAAGGGCTTCCCAGGACCCCCAGGGAGACCAGGACCACCTGGTCCCGCAGGTGC CCCAGGAAGAGCTGCTAAAGGTGACATTCCTGATCCAGGTCCACCTGGAGACCGGGGACCTCCTGGCCCC GATGGCCCAAGAGGAGTACCTGGGCCTCCGGGTTCCCCTGGGAATGTTGACCTTCTGAAAGGGGACCCAG GTGACTGTGGTTTGCCAGGACCACCTGGCTCCCGAGGCCCACCAGGCCCTCCAGGGTGTCAGGGTCCCCC AGGATGTGATGGCAAAGATGGCCAGAAAGGACCAATGGGACTTCCAGGGTTGCCAGGGCCACCTGGTCTT CCTGGGGCACCTGGCGAGAAGGGATTACCTGGCCCTCCAGGCAGAAAAGGGCCAGTAGGTCCTCCAGGCT GCAGAGGTGAACCTGGGCCTCCTGCAGACGTGGATTCCTGTCCTCGAATCCCAGGACTTCCTGGAGTGCC AGGTCCCAGAGGACCAGAAGGAGCTATGGGGGAACCTGGACGGAGAGGCCTGCCTGGACCAGGGTGCAAA GGAGAGCCTGGACCGGATGGCAGACGGGGCCAGGATGGCATCCCTGGATCTCCCGGGCCTCCTGGACGCA AAGGTGACACTGGAGAGGCAGGGTGTCCTGGAGCACCAGGCCCTCCTGGTCCAACTGGTGATCCTGGGCC CAAGGGGTTTGGCCCTGGATCGCTCAGTGGCTTCCTCCTGGTTCTCCACAGTCAGACTGACCAGGAACCG GCCTGCCCCGTGGGCATGCCTCGGCTTTGGACAGGGTACAGCTTGTTGTACATGGAAGGACAGGAGAAGG CGCACAATCAAGATCTTGGTTTGGCAGGCTCTTGTCTTCCTGTGTTTAGCACTCTGCCCTTTGCCTACTG CAACATCCACCAAGTGTGCCACTACGCCCAGAGAAATGACAGGTCCTACTGGCTGTCCAGTGCGGCTCCT CTTCCTATGATGCCACTCTCAGAGGAAGAGATCCGCTCTTACATCAGCCGCTGTGCGGTGTGTGAGGCCC CAGCACAGGCCGTGGCGGTCCACAGTCAGGACCAATCCATCCCACCGTGTCCACGGACATGGAGGAGTCT CTGGATTGGGTACTCATTTCTGATGCACACTGGAGCTGGGGACCAAGGAGGTGGGCAGGCACTGATGTCA CCTGGCAGCTGCCTAGAGGACTTCAGAGCTGCACCGTTCGTTGAATGCCAGGGCCGACAGGGAACCTGTC ACTTTTTTGCAAACGAGTACAGCTTCTGGCTGACCACCGTGAACCCAGACCTGCAGTTTGCCTCTGGCCC GTCACCAGACACCTTGAAAGAAGTTCAGGCCCAGCGCCGGAAAATCAGCAGGTGCCAGGTTTGCATGAAG CACAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007735
Insert Size	5049 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007735.2, NP_031761.1
RefSeq Size	7808 bp
RefSeq ORF	5049 bp
Locus ID	12829
UniProt ID	Q9QZR9
Gene Summary	Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.[UniProtKB/Swiss-Prot Function]

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