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Dgkh (NM_001081336) Mouse Untagged Clone
CAT＃: MC223782
Dgkh (untagged) - Mouse diacylglycerol kinase, eta (Dgkh), (10ug)
CNY 14,920.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5930402B05Rik; D130015C16; DGK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223782 representing NM_001081336
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGGCCGGCAGCCAGCACCACCCTCAGGGCGTCGCGGGAGGAGCGGTCGCTGGGGCCAGCGCGG TTTCCCCCACTGCTGCAGGACCGGGAGAGGATTCGTCCGACAGTGAAGCGGAGCAGGAGGGGCCCCAGAA GCTGATCCGTAAAGTGTCCACCTCTGGACAGATGCGGACCAAGACTAGTATTAAGGAAGGACAACTGCTG AAGCAAACCAGCTCTTTCCAAAGGTGGAAGAAGAGATACTTCAAGCTTCGCGGCCGCACACTTTATTATG CGAAAGACTCCAAGTCCTTGATATTTGATGAAGTTGACCTCTCAGATGCCAGTGTTGCGGAAGCAAGCAC AAAAAATGCCAACAACAGCTTTACGATCATCACTCCGTTCAGAAGACTGATGCTATGTGCTGAGAACAGG AAAGAGATGGAGGTCTGGATCAGCTCTCTGAAGTCAGTGCAGAGCAGAGAACCCTATGAGGTGGCCCAGT TCAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCACGCCCGGCCCACCTTCTGTAA CGTGTGCAGAGAGAGTCTCTCTGGAGTGACCTCCCACGGCCTGTCCTGTGAAGTGTGTAAGTTCAAGGCT CATAAAAGATGTGCAGTGAGGGCAACAAACAACTGCAAATGGACCACGCTGGCCTCCATCGGGAAGGACA TCATAGAGGACGAGGATGGTGTTGCCATGCCTCACCAGTGGCTCGAAGGCAACTTGCCTGTGAGTGCCAA GTGTGCAGTCTGTGACAAAACCTGTGGCAGTGTGCTTCGCCTGCAAGATTGGAAATGCCTTTGGTGTAAG ACAATGGTGCACACTGCCTGCAAAGATGTGTACCATCCCGTCTGTCCCCTTGGCCAGTGTAAAGTCTCCA TCATACCTCCGATTGCACTAAATAGCACAGACTCTGATGGTTTCTGTCGAGCAACCTTTTCGTTCTGTGT CAGCCCCCTCTTGGTGTTCGTCAACTCTAAGAGTGGAGATAATCAGGGAGTGAAGTTCCTTCGTCGCTTT AAACAGTTGCTCAACCCAGCTCAGGTGTTTGATTTAATGAACGGAGGGCCTTATTTAGGCTTGAGATTGT TTCAAAAGTTTGACAACTTTCGGATCCTTGTCTGTGGAGGAGATGGAAGTGTAGGCTGGGTTTTGTCAGA GATTGATAAACTGAACTTGCATAAACAGTGCCAGCTTGGGGTGCTACCCTTGGGTACCGGCAATGACCTT GCTCGAGTTCTTGGCTGGGGAGGTTCATATGATGATGACACCCAACTCCCTCAGATACTAGAGAAGCTGG AACGAGCCAGTACCAAAATGCTGGACAGGTGGAGTATAATGACCTATGAGCTCAAATTGCCAGCAAAGTC GTCTCTACTTCCTGAACCTGTGGCAGCAACTGAAGAATTTTACATGACAATTTATGAAGACTCCGTTGCA AACCATCTTACGAAAATCGTCAACTCTGATGAGCATGCGGTGGTCATATCGTCTGCCAAGATACTCTGTG AAACCGTGAAGGACTTCGTTGCCAAAGTGGAGAAGGCACAGGACAGAACACTGGAAAATACAGTAGTAGC CGAAGCCGTGGCCAGTAAATGCTCAGTCCTAAACGAGAAGCTTGAGCAGTTGCTACAAGCCTTGCACGCG GACTCTCAGGCCAGTCGAGTCCCCCCAGGAATCGGCCCTGCCATCCCCGAAGAGGACACCGTGGAGTCCG CCAGTGACGAGTCCTTGGGTGAAAGCAAGGACCAGCTGGTGAATGACATTGGGAAACCCTCCTCCCAGAA AGCCGTGAAGCCCCGGGAGATAATGCTGCGGGCCAACAGTCTAAAGAAAGCGGTGAGGCAAGTCATTGAA GAAGCTGAAAAAGTTATGGATGAGCCAGCAGTGCAGCCCTCTGAGCCAGTCAGTCCGTCTTGTGACTATG ATACAGAAACAGATGAAGCTAAAGAGGACGACGCGAAAGAGTCGCTATCAGCCAAAACCACATCTCAGTC TCCTGATGCCCAGGCAAGCTGTGGCCATCCCCAAACTGACTCTGTCGCTGGTCCAGCCATGGCCACCACC AAAGAAAACCTCCCTGTGCTCAACACTAGAATCATCTGCCCAGGTTTGAGAGCAGGACTTGCTGCCTCCA TTGCTGGGAGTTCTATCATCAACAAAATGTTGCTGGCGAACATCGATCCTTTCGGGGCAACACCGTTTAT TGACCCAGATCTAGACTCACTAGATGGATACTCTGAAAAATGTGTCATGAACAACTACTTTGGGATTGGA TTAGATGCAAAAATTTCATTAGAATTTAATAATAAGAGAGAGGAGCACCCTGAAAAATGCAGAAGTCGAA CTAAAAACTTGATGTGGTACGGAGTCCTTGGGACCCGGGAGCTACTACAGAGATCGTACAAGAATCTGGA GCAGAGGGTTCAACTTGAGTGTGATGGGCAGTATATACCTCTTCCTAGTCTACAAGGCATAGCGGTGTTG AACATCCCTAGCTACGCCGGAGGCACCAACTTCTGGGGCGGAACCAAAGAGGATGATATATTTGCTGCGC CATCCTTTGATGACAAGATTCTAGAAGTTGTCGCAGTGTTTGACAGTGTGCAGATGGCAGTATCAAGGGT CATTAAATTGCAGCATCACCGGATAGCACAGTGCCGTACGGTAAAAATCACGATATTTGGCGACGAGGGA GTCCCAGTGCAGGTGGATGGTGAAGCATGGGTCCAGCCTCCAGGGATCATCAAGATTGTGCACAAAAACA GAGCTCAGATGCTAACCAGAGACAGAGCCTTTGAAAGCACCCTGAAGTCTTGGGAAGACAAGCAGAAGTG TGACTCTGGGAAGCCAGTTCTTCGAACCAACCTGTACATCCATCCCGCCCCTGACCTGGCAACAGAGGAA GTGTCACAGATGCGGCTCTGCTCCCAGGCTGCGGAGGAACTCATCACTAGGATTTGTGACGCAGCCACGA TTCACTGTCTCCTGGAGCAGGAGCTGGCACATGCAGTGAATGCCTGCTCACACGCCCTGAACAAAGCCAA CCCACGCTTCCCGGAGAGCCTTACGAGAGACACTGCCACCGAGATAGCCATCAACGTCAAGGCGCTCTAT AATGAGACAGAGGCTTTGCTAGTTGGCAGGGTCCCTTTGCACTTGGAATCTCCTCACGAAGAGCGGGTGT CCAGTGCCTTACACTCTGTGGAGATGGAGCTTCAGAAGTTAACGGAGATTCCCTGGCTTTATTACATCCT CCGCCCCAGTGAGGACGAGGAGCCTCCTCTGGATTGTACCAAAAGAAACAACAAAAGCACAGTCTTTCGC ATAGTGCCAAAGTTTAAAAAGGAAAAGGCTCAGAAACAGAAAACAAGTTCGCAGCCTGGACCTGGGGATT CCGAAAGTGGGTCATATGAAGCGAATTCTCCAGGGAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001081336 |
| Insert Size | 3471 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001081336.1, NP_001074805.1 |
| RefSeq Size | 3967 bp |
| RefSeq ORF | 3543 bp |
| Locus ID | 380921 |
| UniProt ID | D3YXJ0 |
| Gene Summary | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:27643686). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcripts and orthologous alignments from human (NM_001204504.1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222176 | Dgkh (tGFP-tagged) - Mouse diacylglycerol kinase eta (Dgkh), (10ug) |
CNY 10,550.00 |
|
| MR222176 | Dgkh (Myc-DDK-tagged) - Mouse diacylglycerol kinase, eta (Dgkh) |
CNY 8,048.00 |
|
| MR222176L3 | Lenti ORF clone of Dgkh (Myc-DDK-tagged) - Mouse diacylglycerol kinase, eta (Dgkh) |
CNY 11,500.00 |
|
| MR222176L4 | Lenti ORF clone of Dgkh (mGFP-tagged) - Mouse diacylglycerol kinase, eta (Dgkh) |
CNY 11,500.00 |
