Exd1 (NM_172857) Mouse Untagged Clone
CAT#: MC219232
Exd1 (untagged) - Mouse exonuclease 3'-5' domain containing 1 (Exd1), (10ug)
CNY 7,320.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 4932702D22Rik; Exdl1; mExd1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC219232 representing NM_172857
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCCAGCAGTGACTACCATTTCCTCAACCAGATTTTGTGGAGAAGGGTGAAGCTCACACTGGTGT CTGGCATCTTTGAGGGCGTGCTCCAGCACGTGGATCCTAACAAGATTGTTGTCCTGAAAAACGTGAGGAA TGCAGAGTCGGGCCGCAGCGTCCCAGGGGTGAAAGTGTTCTTCGGGCACGAGATCTTGAATGTGGAACTA ATGGATGAAGCAGAAGGTGCATCGGGGGAGAAGGCATCCGCTGTTAGCATAAATACAGAACGAGCTGGAA TGGAGAAAGTGAAAAATGAAGACGTCAATGTCTGTGAGCCGGCTTCACCTGCACCAGAGGTGCCAACCAG CTCTCTCCTCAGTGACCTCAAGTACTGCCCGTCAGAGGAAGAGGAGGTGACATACACAGTCATTGATCAG TTCCAGCAGAAGTTTGGTGCTGCAATGCTCCACATCAAGAAGCAGAGTGTCCTGAGTGTTGCTGCCGAAG GCGCCAATGTGTGTCGCCATGGGAAACTCTGTTGGCTTCAGGTGGCCACAAACAGCCGAGTTTACTTGTT TGATATTTTCCTTCTGGGAAGTCGTGCTTTCAATAATGGACTTCAGATGATCTTAGAAGACAAGAGAATC TTAAAGGTTATCCACGATTGTCGCTGGCTCTCTGACTGCCTGTCTCATCAGTATGGAATCATGCTGAACA ATGTCTTTGATACACAGGTAGCCGACGTCCTTCAATTTTCTATGGAAACGGGTGGCTTTCTTCCAAACTG TATCAGTACTTTGCAGGAGAGTTTAATCAGACACCTTAAAGTTGCTCCCAGATACCTTTTCTTTTTAGAA GAGAGACAGAAACGTATTCAGGAAAATCCAGAAATATGGTTGACAAGACCTCTTCCACCCTCTTTATTGA AAATCTTGGCCCTGGAAACAACCTACCTGCTTCCACTTCGATTGGTGCTCCTGGATGAGGTGATGTCAGA CTTAACCACACTGGTGGATGGGTACCTGAACACCTACCGGGAGGGCTCTGCAGACCGGCTTGCAGGCACA GAGCCTGCGTGTATGGAGCTGCCAGCAGAGCTTCTTCAACTCCAGGACTTCCAGAAACAGCGCAGAGAGC GAGCTGTGAAGGAGTACAGAGTGAACGCACGGGGCCTCCTCATAAGGACTCCACTGCACCCAAAGGAGCC GACAGCTTGCACAGCAGGGAAAGAGGAGCGAGTCCAGGGTTTCTTATTTTATAAGACGGATGGTGGAGAC CAAGTTCCACGCTTTTTATGTCCCAAATCTCATGAGGATGAGAAGTTTTTGGATAAAGAATCTAAACAAA CCACAGCAAAGTCTCAGATTGTGCCTCCCAGGAAGGAAGGCGAAGCCCATAAGGACTCTAAGAACAAACC GGGGTGTTGGGAGTCAGCGGGGCCAGAAGACCCGAGAGCCCAGAAAGCCCACGCCCTGCCTCCCACGTGG GCATCTCAGTCCCAGTTTTCTTTGAAAGAGGAGATAGAGCAGTTGACAGTGGTGGGAAATAAGGGAGCTT TAACAAGCCCAAAAGAAGGCGCTTTGGTGTCGCCTTCTCTCCTTCAGGAAACCTGGGAGGCACCAACTGA CACCTTCCATCTTCCTGAAAAAGCTGAGGTCTCTACACTTCCTCCCTGTCCAGCCCTGGAGAAGACAGAC TCATGGATAAGTCCATCTTTAAATCTGTTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_172857 |
Insert Size | 1713 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_172857.2, NP_766445.1 |
RefSeq Size | 3143 bp |
RefSeq ORF | 1713 bp |
Locus ID | 241624 |
UniProt ID | Q8CDF7 |
Gene Summary | RNA-binding component of the PET complex, a multiprotein complex required for the processing of piRNAs during spermatogenesis. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposable elements, preventing their mobilization, which is essential for the germline integrity (PubMed:26669262). The PET complex is required during the secondary piRNAs metabolic process for the PIWIL2 slicing-triggered loading of PIWIL4 piRNAs. In the PET complex, EXD1 probably acts as an RNA adapter. EXD1 is an inactive exonuclease (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG219928 | Exd1 (tGFP-tagged) - Mouse exonuclease 3'-5' domain containing 1 (Exd1), (10ug) |
CNY 5,040.00 |
|
MR219928 | Exd1 (Myc-DDK-tagged) - Mouse exonuclease 3'-5' domain containing 1 (Exd1) |
CNY 4,248.00 |
|
MR219928L3 | Lenti ORF clone of Exd1 (Myc-DDK-tagged) - Mouse exonuclease 3'-5' domain containing 1 (Exd1) |
CNY 6,460.00 |
|
MR219928L4 | Lenti ORF clone of Exd1 (mGFP-tagged) - Mouse exonuclease 3'-5' domain containing 1 (Exd1) |
CNY 6,460.00 |