Wipi1 (NM_145940) Mouse Untagged Clone
CAT#: MC215904
Wipi1 (untagged) - Mouse WD repeat domain, phosphoinositide interacting 1 (Wipi1), (10ug)
CNY 5,800.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 4930533H01Rik; AW411817; D11Ertd498e |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC215904 representing NM_145940
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCGAAGCCGCAGATGCCCCTCCGGGCCGAGTGGAGGCGGCGCTCAGCTGCTTCTCTTTCAACC AAGACTGCACATCCCTAGCGATTGGAACCAAGGCCGGTTACAAGCTGTTTTCTCTGAGTTCTGTGGAGCA GCTTGACCAAGTCCATGGAAGCAATGAAATCCCTGACGTGTATATCGTGGAGCGCCTCTTCTCCAGCAGC CTGGTTGTAGTGGTCAGTCACACAAAACCTCGGCAGATGAACGTCTACCATTTCAAGAAAGGCACTGAGA TCTGTAATTACAGCTACTCCAGCAACATTTTGTCTATTCGGCTCAACCGACAGAGGCTGCTGGTCTGCCT GGAAGAATCCATCTATATCCACAACATTAAGGATATGAAGTTATTGAAGACCGTCCTGGATATTCCCTCA AACCCAACAGGTCTCTGTGCCCTGTCTATCAACCATTCCAACTCTTACCTGGCCTATCCTGGAAGCCAGA GTACAGGCGAGATTGTACTCTATGATGGAAACTCCCTGAAAACGGTGTGCACCATTGCTGCCCACGAGGG GACGCTGGCCGCTATCACCTTCAACTCCTCGGGCTCCAAGCTAGCAAGCGCGTCTGAAAAAGGCACTGTC ATCCGAGTGTTCTCTGTTCCCGAGGGCCAGAAACTCTATGAGTTTCGTCGAGGAATGAAAAGGTATGTGA CAATCAGCTCCCTGGTGTTCAGTATGGACTCCCAGTTCCTGTGTGCCTCCAGCAACACGGAGACCGTGCA CATCTTCAAGATGGAACACCTGACAGACAGCCGCCCAGAAGAGCCTTCCACCTGGAGCGGCTACATGGGA AAGATGTTCATGGCAGCTACCAACTACCTCCCCGCCCAGGTGTCGGACATGATGAACCAGGACAGGGCTT TCGCCACAGGACGCCTGAACTTCTCTGGGCAGAAGAACATTTGCACCCTGTCCACGATCCAGAAACTGCC GCGGTTGCTGGTGGCCTCCTCCGACGGACACCTTTACATCTACAACTTGGACCCACAGGATGGAGGAGAA TGTGTCCTAATCAAAACCCACAGCTTGCTTAGCTCAGGAACAACAGAAGAGAACAAAGAAAATGACCTCA GACCTTCCTTACCTCCATCTTATGCTGCAACTGTAGCAAGGCCCAGCACGTCTGCAGCCTCCACGGTGCC AGGATACTCTGAGGACGGCGGGGCGCTCCGAGGGGAAGTTATTCCGGAACACGAGTTTGCGACGGGACCA GTGTGTCTAGACGACGAGAATGAGTTTCCCCCTATAATCTTGTGCCGTGGAAGTCAGAAGGGCAAAACGA AGCAGTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_145940 |
Insert Size | 1341 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_145940.2, NP_666052.1 |
RefSeq Size | 1817 bp |
RefSeq ORF | 1341 bp |
Locus ID | 52639 |
UniProt ID | Q8R3E3 |
Gene Summary | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy (By similarity) (PubMed:22275429). Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG207122 | Wipi1 (tGFP-tagged) - Mouse WD repeat domain, phosphoinositide interacting 1 (Wipi1) |
CNY 4,370.00 |
|
MR207122 | Wipi1 (Myc-DDK-tagged) - Mouse WD repeat domain, phosphoinositide interacting 1 (Wipi1) |
CNY 3,656.00 |
|
MR207122L3 | Lenti ORF clone of Wipi1 (Myc-DDK-tagged) - Mouse WD repeat domain, phosphoinositide interacting 1 (Wipi1) |
CNY 5,890.00 |
|
MR207122L4 | Lenti ORF clone of Wipi1 (mGFP-tagged) - Mouse WD repeat domain, phosphoinositide interacting 1 (Wipi1) |
CNY 5,890.00 |