Chrna5 (NM_176844) Mouse Untagged Clone

CAT#: MC215858

Chrna5 (untagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 5 (Chrna5), (10ug)



  "NM_176844" in other vectors (4)

CNY 3,656.00

CNY 5,700.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
CHRNA5 Antibody - middle region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Acra-5; Acra5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC215858 representing NM_176844
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGATCTCGAATGCAGGGTTGCCTGAGCTATCCTCTGCTGCAAAACATGAAGACAGTTTGTTTAGGG
ATTTATTTGAAGACTATGAAAAGTGGGTTCGTCCTGTGGAACACCTGAGTGACAAGATAAAAATCAAGTT
TGGCCTTGCAATATCTCAGTTAGTGGACGTGGATGAGAAAAACCAGCTAATGACCACCAACGTCTGGTTG
AAGCAGGAATGGATAGATGTAAAGTTAAGATGGAATCCTGATGACTATGGTGGAATAAAAATTATACGTG
TTCCTTCAGACTCTCTGTGGATCCCAGACATCGTTTTGTTTGATAATGCAGACGGACGTTTCGAAGGGGC
CAGTACGAAAACAGTTGTCAGGTACAATGGCACTGTCACTTGGACGCAGCCAGCAAACTACAAAAGTTCT
TGCACTATAGATGTGACCTTTTTCCCATTTGATCTCCAAAATTGCTCCATGAAATTCGGCTCATGGACGT
ATGATGGATCCCAGGTCGATATAATCCTAGAGGACCAAGATGTCGACAGAACAGACTTTTTTGACAATGG
AGAATGGGAAATCATGAGCGCAATGGGGAGCAAGGGGAACCGGACGGACAGCTGCTGCTGGTACCCCTGC
ATCACCTACTCCTTCGTGATCAAACGGCTGCCTCTCTTCTACACCCTGTTTCTTATCATACCCTGCATCG
GGCTCTCGTTTCTGACTGTGGTTGTCTTCTATCTCCCTTCAAACGAAGGTGAAAAGATTAGCCTGTGCAC
TTCCGTGCTGGTCTCTCTGACTGTCTTCCTGCTGGTGATTGAGGAAATTATACCGTCATCTTCCAAAGTC
ATACCTCTGATCGGGGAGTACTTGGTGTTCACCATGATCTTCGTGACCCTATCCATTATGGTGACTGTCT
TTGCCATCAACATCCACCACCGCTCTTCTTCCACACACAATGCTATGGCGCCGTGGGTTCGTAAGATATT
TCTCCACAAGCTTCCCAAACTGCTCTGCATGAGAAGTCATGCCGATAGGTACTTCACTCAGAGAGAAGAA
GCCGAGAAAGACGGTGGACCTAAATCTCGGAATACTTTGGAGGCCGCGCTCGATTGCATTCGCTACATCA
CGAGACACGTAGTGAAAGAGAACGACGTCCGCGAGGTTGTTGAAGATTGGAAATTCATAGCCCAAGTTCT
CGATCGGATGTTTCTATGGACGTTTCTTCTGGTTTCAATCATTGGGACGCTAGGGCTTTTTGTTCCTGTT
ATTTATAAATGGGCCAATATAATAGTCCCAGTTCACATTGGAAACACAATTAAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_176844
Insert Size 1317 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_176844.4, NP_789814.2
RefSeq Size 2738 bp
RefSeq ORF 1317 bp
Locus ID 110835
UniProt ID Q2MKA5
Gene Summary After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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