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Spout1 (NM_172660) Mouse Untagged Clone
CAT＃: MC212809
D2Wsu81e (untagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (D2Wsu81e), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9930028P05; AL033358; CENP-32; D2Wsu81e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC212809 representing NM_172660
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCGCCCGAGGAAGCGTCCGTGTGGTCCGGGTGAACATGGCCAAAGGGTTGAATGGCGAAAAT GGAAACAGCAGAAAAAAGAGGAGAAAAAGAAGTGGAAGGACCTCAAGATAATGAAGAAACTGGAGCGGCA GCGTGCCCAAGAGGAAGAGGCTAAACGCCAGGAAGAGGAGGAAGAGGCGGCGGCCCAGAGGAGCAACCAA GGACGGCCTTACACTCTGAGTGTGGCCCTGCCAGGCTCCATCCTGGACAATGCCCAGTCACCTGAGCTCC GCACCTACCTGGCTGGCCAGATCGCCAGAGCCTGCACCATCTTCTGTGTGGATGAGATTGTGGTGTTCGA TGAGGAGGGCCAAGATACCAAGAGTGTGGAAGGAGAATTCAGGGGAGTTGGCAAGAAAGGGCAGGCATGT GTCCAGCTGGCCCGCATCCTACAGTACCTGGAGTGTCCACAGTACCTGAGAAAGGCGTTCTTCCCCAAGC ACCAAGATCTGCAGTTTGCAGGAATCCTCAACCCCTTGGACAGCCCTCACCACATGCGTCAGGACGAAGA GTCTGAGTTTCGAGAAGGCGTCGTGGTGGACCGGCCCACCAAGGCAGGCCATGGCTCTTTGGTCAACTGT GGAATGAAGAAGGAGGTGAAGATTGACAAGAAACTGGACCCTGGACTTCGGGTGACCGTGCGACTGAACC AGCAGCAGCTCCCAGAATGCAAGACATACAAAGGAACAGTTGTGTCATCACAGGACCCCCGCACCAAAGC TGGTCTCTACTGGGGCTACACTGTCCGGCTGGCCTCTTGCCTCAGCGCTGTGTTTGCTGAGGCTCCCTTC CAGGATGGGTACGACCTGACCATTGGGACATCAGAACGTGGCTCCGACGTGGCCTCTGCCCAGCTGCCCA GCTTCAGGCATGCCCTTGTGGTGTTTGGAGGCCTCCAAGGGCTAGAAGCTGCAGTGGATGCTGACCCGAA CCTTGAAGTGGCTGACCCCAGTGTCCTCTTCGACTTCTATGTCAACACATGCCTTAGCCAGGGCAGCCGT ACCATCCGCACCGAGGAGGCCATACTCATCTCGCTGGCTGCTCTGCAGCCTGGTCTCACCCAGGTGGGAT CGAGGCCCGCCTCCCCGCTTTCTGGACCCAGGATGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_172660 |
| Insert Size | 1158 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172660.4, NP_766248.3 |
| RefSeq Size | 1838 bp |
| RefSeq ORF | 1158 bp |
| Locus ID | 227695 |
| UniProt ID | Q3UHX9 |
| Gene Summary | Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis. Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225943 | D2Wsu81e (tGFP-tagged) - Mouse DNA segment Chr 2 Wayne State University 81 expressed (D2Wsu81e), (10ug) |
CNY 4,180.00 |
|
| MR225943 | D2Wsu81e (Myc-DDK-tagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (D2Wsu81e) |
CNY 3,800.00 |
|
| MR225943L3 | Lenti ORF clone of D2Wsu81e (Myc-DDK-tagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (D2Wsu81e) |
CNY 5,700.00 |
|
| MR225943L4 | Lenti ORF clone of D2Wsu81e (mGFP-tagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (D2Wsu81e) |
CNY 5,700.00 |
