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Trmu (NM_028063) Mouse Untagged Clone
CAT＃: MC211275
Trmu (untagged) - Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu), nuclear gene encoding mitochondrial protein, (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110005N20Rik; 1600025P05Rik; AI314320; Trmt1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC211275 representing NM_028063
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGCTGCGGCACGTCGTGTGCGCCCTGTCTGGCGGTGTGGACAGCGCGGTGGCGGCGCTGCTGT TGCGGCGGAGAGGTTACCAGGTGACAGGGGTATTTATGAAGAACTGGGACTCGCTGGATGAGCAGGGCGT TTGTGCTGCTGACAAGGACTGTGAAGATGCGTATAAAGTTTGTCAGATCTTAGACATCCCTTTCCACCAG GTGTCCTATGTGAAGGAGTATTGGAATGATGTGTTCAGTGACTTTTTGAACGAGTATGAGAAAGGAAGGA CTCCAAACCCCGACATCAACTGCAATAAGCATATCAAGTTCAGTTGCTTTTATCACTATGCTGTGGACAA TCTTGGAGCAGATGCCGTTGCCACTGGCCACTACGCCAGGACCTCACTGGAGGATGAGGAAGTCTTTGAG CAAAAGCACACTAAGAAGCCTGACGGGCTGTTCAGAAATCGATTTGAAGTCAGAAACCCCGTAAAACTTC TCCAAGCAGCTGACAGCTTTAAAGACCAGACCTTCTTTCTCAGCCAGGTTTCCCAGGATGCCCTGAGAAG GACCATCTTTCCCCTGGGGGAACTAACAAAAGATTTTGTAAAGAAAATAGCCGCGGAGAATAGCCTCCAT CACGTGCTTCAGAAGAGAGAGAGCATGGGCATCTGCTTCATTGGTAAGAGAAACTTGGAGCATTTCCTTC TTCAGTATTTGCAGCCTCGGCCTGGAAAGTTTGTTTCTATCGAGGATAATACAGTTCTGGGAACACACAA AGGTTGGTTCCTGTACACTTTGGGCCAGAGAGCTAAGATCAGCGGCTTGAGAGAGCCCTGGTACGTGGTG GAGAAGGATGGCACCAAGGGTGACGTGCTTGTGGCTCCCCGGGTGGACCACCCAGCTCTGTACAGGGACC TGCTCAGGACCAACCGAGTACACTGGATTGCGGAGGAGCCACCTGCTGCCTTGGTCAGAGACAAGATGAT GGAGTGCCATTTCCGATTCCGCCACCAGATGGCACTAGTGCCCTGCGTGCTGACTCTCAATCAGGATGGC ACTGTGTGGGTAACGGCTGTGAAGGCTGTGAGGGGCCTTGCCCTGGGACAGTTTGCAGTGTTCTACAAGG GTGAGGAGTGCCTCGGCAGTGGGAAAATCCTGCGGCTAGGGCCGTCTGCCTACACACTCCAGAAGGGTAA GAACAGAACTCGAGTGGCTCCCGAGGCCTCCAGTGACAGCCCAGGCCTGCACCCCACATCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_028063 |
| Insert Size | 1254 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_028063.2, NP_082339.1 |
| RefSeq Size | 1520 bp |
| RefSeq ORF | 1254 bp |
| Locus ID | 72026 |
| UniProt ID | Q9DAT5 |
| Gene Summary | Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206594 | Trmu (tGFP-tagged) - Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu) |
CNY 3,710.00 |
|
| MR206594 | Trmu (Myc-DDK-tagged) - Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
| MR206594L3 | Lenti ORF clone of Trmu (Myc-DDK-tagged) - Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
|
| MR206594L4 | Lenti ORF clone of Trmu (mGFP-tagged) - Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
