Tpm1 (NM_001164250) Mouse Untagged Clone
CAT#: MC209577
Tpm1 (untagged) - Mouse tropomyosin 1, alpha (Tpm1), transcript variant 4, (10ug)
CNY 3,990.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AA986836; AI854628; alpha-TM; TM2; Tm3; Tmpa; Tpm-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC209577 representing NM_001164250
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCCATCAAGAAGAAGATGCAGATGCTGAAGCTCGACAAAGAGAACGCCTTGGATCGAGCTGAGC AAGCGGAGGCTGATAAGAAGGCGGCGGAAGACCGGAGCAAGCAGCTGGAAGATGAGCTGGTGTCACTGCA AAAGAAACTCAAGGGCACTGAAGATGAACTGGACAAATACTCCGAGGCTCTCAAAGATGCCCAGGAGAAA CTGGAGCTGGCGGAGAAAAAGGCCACAGATGCTGAAGCTGACGTAGCTTCTCTGAACAGACGCATCCAGC TGGTTGAGGAGGAGTTGGATCGTGCTCAGGAGCGTCTGGCCACAGCTCTGCAGAAGCTGGAGGAGGCCGA GAAGGCTGCAGATGAGAGTGAGAGAGGCATGAAAGTCATTGAAAGCCGAGCCCAAAAAGATGAAGAAAAG ATGGAGATTCAGGAGATCCAGCTGAAAGAGGCCAAGCACATTGCTGAAGATGCTGACCGGAAGTATGAAG AGGTGGCCCGTAAGCTGGTCATCATCGAGAGCGACCTGGAACGTGCAGAGGAGCGGGCTGAGCTCTCAGA AGGCCAAGTTCGACAGCTGGAAGAACAGTTAAGAATAATGGATCAGACCTTGAAAGCATTAATGGCTGCA GAGGATAAGTACTCTCAGAAGGAAGACAAATATGAAGAGGAGATCAAGGTTCTCTCTGACAAGCTGAAGG AGGCTGAAACTCGGGCTGAGTTTGCAGAGAGATCAGTAACCAAATTGGAGAAAAGCATTGATGACTTAGA AGAGAAAGTGGCCCATGCCAAAGAAGAAAACCTTAGTATGCACCAGATGCTGGATCAGACTTTACTGGAG CTAAACAACATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001164250 |
Insert Size | 855 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001164250.1, NP_001157722.1 |
RefSeq Size | 1702 bp |
RefSeq ORF | 855 bp |
Locus ID | 22003 |
Gene Summary | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (Tpm1.7, also known as variant 4) lacks an exon and contains another alternate exon compared to variant Tpm1.6. The encoded isoform (Tpm1.7cy) is the same length but has short regions of sequence differences and an alternate C-terminus, compared to isoform Tpm1.6cy. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG219100 | Tpm1 (tGFP-tagged) - Mouse tropomyosin 1 alpha (Tpm1) transcript variant 4, (10ug) |
CNY 3,140.00 |
|
MR219100 | Tpm1 (Myc-DDK-tagged) - Mouse tropomyosin 1, alpha (Tpm1), transcript variant 4 |
CNY 2,850.00 |
|
MR219100L3 | Lenti ORF clone of Tpm1 (Myc-DDK-tagged) - Mouse tropomyosin 1, alpha (Tpm1), transcript variant 4 |
CNY 4,750.00 |
|
MR219100L4 | Lenti ORF clone of Tpm1 (mGFP-tagged) - Mouse tropomyosin 1, alpha (Tpm1), transcript variant 4 |
CNY 4,750.00 |