Epm2a (NM_010146) Mouse Untagged Clone
CAT#: MC208444
Epm2a (untagged) - Mouse epilepsy, progressive myoclonic epilepsy, type 2 gene alpha (Epm2a), (10ug)
CNY 3,990.00
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | TcrbK)TG-BFlv; Tg(TcraK; TG-B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC208444 representing NM_010146
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTTCCGCTTCGGCGTGGTGGTGCCGCCCGCGGTGGCCGGCGCTCGGCAGGAGCTGCTGCTCGCCG GCTCGCGGCCCGAGTTAGGGCGCTGGGAGCCGCACGGCGCCGTGCGTCTCAGGCCCGCGGGTACCGCGGC GGGCGCCGCTGCGCTGGCGCTGCAGGAGCCCGGCCTGTGGCTCGCCGAGGTGGAGCTGGAGGCGTACGAG GAGGCCGGCGGGGCGGAGCCGGGCCGCGTTGACACGTTCTGGTACAAGTTCCTGCAGCGCGAGCCTGGAG GCGAGCTGCACTGGGAAGGAAATGGACCTCACCATGACCGTTGCTGCACATATAATGAGGACAACTTGGT GGATGGTGTTTATTGTCTCCCAGTAGGACACTGGATTGAGGCCACTGGGCACACCAATGAAATGAAGCAC ACAACAGACTTCTATTTTAATATTGCTGGCCACCAAGCCATGCACTATTCAAGAATTCTACCAAATATCT GGCTGGGTAGCTGCCCTCGCCAACTGGAACATGTGACCATCAAACTGAAGCATGAACTGGGAGTTACAGC TGTCATGAATTTCCAGACTGAATGGGATATCATCCAGAATTCTTCAGGCTGCAACCGCTACCCTGAACCC ATGACTCCAGACACCATGATGAAGCTGTATAAGGAAGAAGGCTTGTCCTACATCTGGATGCCCACTCCAG ACATGAGCACTGAGGGCCGAGTGCAGATGCTGCCACAGGCTGTGTGTCTCCTGCACGCGCTTCTGGAGAA TGGACACACGGTGTATGTCCACTGCAACGCTGGCGTGGGTCGCTCCACAGCTGCAGTGTGCGGCTGGCTC CACTATGTGATTGGCTGGAATCTGCGCAAGGTGCAGTACTTCATCATGGCCAAGAGGCCTGCGGTCTACA TTGACGAGGACGCTTTGGCTCAAGCACAACAAGACTTTTCTCAGAAGTTCGGGAAGGTTCACTCTTCCAT ATGCGCTTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_010146 |
Insert Size | 993 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_010146.2, NP_034276.2 |
RefSeq Size | 1079 bp |
RefSeq ORF | 993 bp |
Locus ID | 13853 |
UniProt ID | Q9WUA5 |
Gene Summary | Plays an important role in preventing glycogen hyperphosphorylation and the formation of insoluble aggregates, via its activity as glycogen phosphatase, and by promoting the ubiquitination of proteins involved in glycogen metabolism via its interaction with the E3 ubiquitin ligase NHLRC1/malin (PubMed:18040046, PubMed:18852261, PubMed:19036738, PubMed:23663739, PubMed:24430976, PubMed:24068615). Dephosphorylates phosphotyrosine and synthetic substrates, such as para-nitrophenylphosphate (pNPP), and has low activity with phosphoserine and phosphothreonine substrates (in vitro) (PubMed:16971387, PubMed:24430976). Has also been shown to dephosphorylate MAPT (PubMed:19542233). Shows strong phosphatase activity towards complex carbohydrates in vitro, avoiding glycogen hyperphosphorylation which is associated with reduced branching and formation of insoluble aggregates (PubMed:18040046, PubMed:18852261, PubMed:23663739). Forms a complex with NHLRC1/malin and HSP70, which suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS) (PubMed:19036738, PubMed:24068615). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Also promotes proteasome-independent protein degradation through the macroautophagy pathway (PubMed:20453062).[UniProtKB/Swiss-Prot Function] |
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Other Versions
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MG222730 | Epm2a (tGFP-tagged) - Mouse epilepsy progressive myoclonic epilepsy type 2 gene alpha (Epm2a), (10ug) |
CNY 2,850.00 |
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MR222730 | Epm2a (Myc-DDK-tagged) - Mouse epilepsy, progressive myoclonic epilepsy, type 2 gene alpha (Epm2a) |
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MR222730L3 | Lenti ORF clone of Epm2a (Myc-DDK-tagged) - Mouse epilepsy, progressive myoclonic epilepsy, type 2 gene alpha (Epm2a) |
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MR222730L4 | Lenti ORF clone of Epm2a (mGFP-tagged) - Mouse epilepsy, progressive myoclonic epilepsy, type 2 gene alpha (Epm2a) |
CNY 4,750.00 |