Rras2 (BC003871) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610016H24Rik; C86394; TC21
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003871 ATCCGCGAAGAAGAGCGCGCTCCCTGGCCGCTGCGTCCTCTGCCCTGGAGCCTTCCCGGCTCTCCAGCGT CTCGGGCAACTCCCAGCCGGTCCGAGGGATAGCGCTCCGCGGCCCTCCAGGCCACTCACGGACCCCGCGC GCTCTACCGCCTGTAACCCAGCCGGCCGCACTCGTCCCCGGAGCCGGGCCTAGGGCGCGCGGCGGCCGGG CTGGGCTCGGGGCGTCCCGATGGCCGCGGCCGGCTGGCGCGACGGCTCCGGCCAGGAGAAGTACCGGCTC GTGGTGGTCGGCGGTGGCGGCGTGGGCAAGTCGGCGCTCACCATCCAGTTCATCCAGTCCTATTTTGTGA CGGACTATGATCCAACCATTGAAGACTCATACACAAAGCAATGTGTGATCGATGACCGAGCTGCCCGGCT GGACATTTTGGATACAGCCGGACAAGAGGAGTTTGGAGCCATGAGAGAACAATACATGAGGACAGGCGAG GGCTTCCTGTTGGTCTTCTCAGTCACGGATAGAGGCAGTTTTGAAGAAATCTATAAGTTTCAAAGACAGA TTCTCAGAGTAAAGGACCGTGATGAGTTTCCCATGATTTTAATTGGCAATAAAGCTGACCTGGACCATCA GAGACAGGTAACACAGGAAGAAGGGCAGCAGTTAGCAAGGCAGCTCAAGGTCACATATATGGAGGCATCG GCAAAGATCAGGATGAATGTAGACCAAGCTTTCCACGAACTTGTCCGGGTTATAAGGAAATTTCAAGAGC AAGAATGCCCTCCTTCACCAGAACCAACCCGGAAAGAAAAAGACAAGAAAGGCTGCCATTGTGTCATTTT CTAAGAGTCCCTTGAGGTTTAGCTAACAACTGCCAGGGAAGCCCTCATCTCTGCTACCGTTTACATCATC CGGTACCTTTGTAGCCTTAGACCGATGATCACCGTGTTAGCCTTACACCAAGAAGCTGGCAAATCCTTTA TGTGAAGCTAATGTAACGGTCTTACCCAGACTTGTGTAAAGGAAACACTAAGGCTGCTTCAGACGTTATC TGATTCCTTTAAAACACATGTCTACACACACAGATGCAGTCTTCAAGGGCTTTTAATAGAGGTAAACCAA GTTTGGAGCCTGAGCTGTTTGCTAAGCTGAAGTCATAGGTTGGAAAGTAATTTTTAACTTCTGGAATCAT GTTGCCTACTGTTACTCTAAATAGAAATATAGGGAAGGTTTTTTTTTTGTTGTTGTTGTTTTTTTAAATG TGAATTTTTGCCTATCTTTAAAACTTTGGTTGTCAACTTTCATTAACCTTAAATACACTGAATGGAATCA ACACAAGTGAGACATCTCAGACCTTTCCTGATGCTACGGCTTTTGTTTTTCCAGTGGCCAAAATACCAAA ATGCCTGTTGTATTTATGGATTAAAAACTGCTGATTAAGCCTTTGTTATCATGCTTCCTTCAAGATGACA ATATTCTCTGTGGCCAAATGTTTGGACTGGTTCTGCACTTGGGTGTTTTGGTGAACTAGCTATCTTTTCA CAGCCGTGGTAAGGGTAAGAGTGGATGGTTCCTGTCTGTCTTCCTTCTCAAGTATTCCTGGTTAAGGGAT TAAAAAAGAAAACCCACACAAGCTAAAGCTGTTTTTGTTTGATGTAATATAAAATATGGAATTGATCTTT CCAAAGTCAGAGATGATTAATGTTTTTGCTATATACTTTTATACGTTATTTTCTTATCAAACTAGTTAAC AAGTATTTTTATATGTTTGTAAGCGGATATGCTTCCACCTTGTGTATATGTAAAGATGAGTATTTAAGTC TCACTGTTCACTGTTAACTGACAAAGAGACCATGGCTTTTGCTTGCTGGCTTGGTCCTTTTTACATCACT GGTGGCCAATAATCAGAAAACCTTCCCAACAGAACGCAACAGGACGAGAGTATGAAGTCACGTTCAGTGT CCCCGCTAGATATTGACTGTTATATCAAGTATTACGTGTAAAACCTCTACTTGACAATTAGTGTAACTGT GGATGGCTTCTGACTTTGTTTTTAACAATGTGGATTGTGTTTAAGCAATTCGAAGTGCGCTCCAGATTTT GAGACTCAGTGGTATTGCACAGTGGTCACTTTATTGAATGTGTACAACAGCCCCATGAAGTTTATAAAGT GGACCCTTGTATAGCTTCAGGTGCTAGAATTAAAGTTGATCTGTTGTCACCAGAAAAAAAAAAAAAAAAA AAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC003871
Insert Size	615 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003871, AAH03871
RefSeq Size	2251 bp
RefSeq ORF	615 bp
Locus ID	66922
Gene Summary	It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion.[UniProtKB/Swiss-Prot Function]

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