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Btk (NM_013482) Mouse Untagged Clone
CAT＃: MC202055
Btk (untagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk), (10ug)
CNY 4,912.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI528679; xid |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC053392 sequence for NM_013482
AAGAGGTAATCCAGTCCTGGCATCTCACGACGTCTGGGGAGCTACCTGCATTAAGTCAGAACTGAGTACA CAAACAAGTTCCAGAGAGAGGAAGCCATGGCTGCAGTGATACTGGAGAGCATCTTTCTGAAGCGCTCCCA GCAGAAAAAGAAAACATCACCTTTAAACTTCAAGAAGCGCCTGTTTCTCTTGACTGTACACAAACTTTCA TACTATGAATATGACTTTGAACGTGGGAGAAGAGGCAGTAAGAAAGGTTCAATAGATGTTGAGAAGATCA CCTGTGTTGAAACAGTAATTCCTGAAAAAAATCCCCCACCAGAAAGACAGATTCCGAGGAGAGGTGAGGA GTCTAGTGAAATGGAACAGATTTCAATCATTGAAAGGTTCCCGTACCCATTCCAGGTTGTATATGATGAA GGACCTCTCTATGTTTTCTCCCCAACTGAAGAGCTGAGAAAGCGCTGGATTCACCAGCTCAAAAATGTAA TCCGGTGCAATAGTGACCTGGTACAGAAATACCATCCTTGCTTCTGGATTGATGGACAGTATCTCTGCTG CTCTCAGACAGCCAAGAATGCTATGGGCTGCCAAATTTTGGAGAACAGGAATGGAAGCTTAAAACCTGGG AGTTCTCATCGAAAAACGAAAAAGCCTCTTCCCCCTACCCCAGAGGAAGATCAGATCTTGAAAAAACCGC TTCCCCCGGAGCCAACAGCAGCACCAATCTCCACAACCGAGCTGAAAAAGGTCGTGGCCCTTTATGATTA CATGCCAATGAACGCAAATGACTTACAATTGCGAAAGGGCGAGGAGTATTTTATCCTGGAGGAGAGCAAC CTACCGTGGTGGCGAGCACGAGATAAAAATGGGCAGGAAGGCTACATCCCAAGTAACTATATCACTGAAG CTGAGGACTCCATAGAGATGTATGAGTGGTATTCCAAGCACATGACTCGAAGTCAAGCTGAGCAACTGCT AAAGCAAGAGGGGAAAGAAGGAGGTTTCATTGTCGGAGACTCCAGCAAAGCTGGAAAATACACCGTGTCT GTGTTTGCTAAATCTACTGGGGAGCCTCAAGGGGTGATCCGCCATTACGTTGTGTGTTCCACGCCACAGA GCCAGTATTACCTGGCTGAGAAACACCTCTTCAGCACCATCCCTGAGCTCATTAACTACCATCAACACAA CTCTGCAGGCCTCATATCCAGGCTGAAATATCCTGTGTCTAAACAAAACAAAAACGCGCCTTCTACTGCA GGCCTGGGCTATGGATCATGGGAAATTGATCCAAAGGACCTCACCTTCTTGAAGGAGCTTGGGACTGGAC AATTCGGTGTCGTGAAATATGGGAAGTGGAGGGGCCAATATGATGTGGCCATCAAGATGATCAGAGAAGG TTCCATGTCGGAGGATGAATTCATTGAAGAAGCCAAAGTCATGATGAATCTTTCCCATGAGAAGCTGGTG CAGTTGTATGGCGTCTGCACCAAACAACGCCCCATCTTCATCATCACCGAGTACATGGCTAATGGCTGCC TCTTGAACTACCTGAGGGAGATGCGGCACCGCTTCCAGACACAGCAGCTGCTTGAGATGTGCAAAGATGT CTGTGAAGCAATGGAATACTTGGAGTCGAAGCAGTTCCTTCACAGAGACCTGGCAGCTCGAAACTGTTTG GTAAACGATCAAGGAGTTGTGAAAGTATCTGACTTTGGCCTGTCTAGGTATGTCCTTGATGATGAGTACA CCAGCTCTGTAGGCTCCAAGTTTCCAGTCCGGTGGTCTCCACCAGAAGTGCTTATGTATAGCAAGTTCAG CAGCAAATCTGACATCTGGGCTTTTGGGGTTTTAATGTGGGAGATCTACTCCCTGGGGAAGATGCCGTAT GAGAGATTTACTAACAGTGAGGCAGCAGAACACATTGCTCAAGGCTTACGTCTCTACAGGCCTCATCTGG CATCAGAGAGGGTATATACCATCATGTACAGCTGCTGGCACGAGAAAGCAGATGAACGTCCTAGTTTCAA AATTCTCTTGAGTAACATTCTAGATGTGATGGATGAAGAATCCTGAGCTGGCTGCTAAGCTCCGTGGATC TCCTCCTCTCTCCTACAAAACCTAATTCCATGTTTCCTGAGGAGTTCCCTGGCTGCAGGGCTCTAGCCTT CCATGCGCCTACTGAATGCATGAAGAGCCCTGGACATCTAGGAATGCCTTTCTCCTCTCGTTCCCTGGCA TACTGCTCTAAGCAAAGGTCAAGGGATTTCTGTGCATAGTATTACCCATAACTTCAAGACTCCTAACAGA CTGAATTGGGGATGGGAACACTTTGGGGGAGGGAAAACTGTAAATAGCTCCACTAGTTGTCCAACAGCTT GTTGGTTAAGTGTTAAGAGTGGTGGTGGTGGTGGGGGGGTAGGAATGTTGCCATTAAAATGGGTTGTTTT GTTACAATAAAATTATTGGAAAGAGTAAGAAAAAAAAAAAAAG |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_013482 |
| Insert Size | 1980 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC053392, AAH53392 |
| RefSeq Size | 2493 bp |
| RefSeq ORF | 1980 bp |
| Locus ID | 12229 |
| UniProt ID | P35991 |
| Gene Summary | Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209829 | Btk (tGFP-tagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk) |
CNY 5,040.00 |
|
| MR209829 | Btk (Myc-DDK-tagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk) |
CNY 4,912.00 |
|
| MR209829L3 | Lenti ORF clone of Btk (Myc-DDK-tagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk) |
CNY 6,460.00 |
|
| MR209829L4 | Lenti ORF clone of Btk (mGFP-tagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk) |
CNY 6,460.00 |
