Sult2b1 (BC009813) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI326997; BB173635; ST2B1; SULT2B
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC009813 CCACGCGTCCGCTACCTCCTGCCCTGCCCGCCATGGACGGGCCGCAGCCCCGCGCCCTGTGGAGCTCGTC TGAGAAAAATGTTTCCGAAATGAGCTGGAATTTTGGAGGTGAATACTTCAGATACAAAGGTATCCCTTTT CCTGTCGGCATGTACTCACCGGAGAGCCTCAGTCTGGCTGAGAACACTAGCAACGTGCGGGACGACGACA TCTTCATTGTCACCTACCCCAAATCAGGTACCAACTGGATGATTGAGATCGTCTGCTTAATCCTGAAAGA TGGGGATCCCTCGTGGATCCGATCGGAGCCCATCTGGCAACGTGCGCCCTGGTGCGAGACCATCATAAGC GCCTTCAATGTCTTAGACCGGCCCAGCCCCCGCATTATGAGCTCTCACCTCCCTATTGAACTCTTCACGA AGGCATTCTTCAGCTCCAAGGCTAAGGTGATTTACGTGGGCCGGAACCCCCGCGATGTCGTGGTCTCCCT CTATTATTATTCTAAGATTGCTGGGCAATTAAAGGACCCTGGTACACCCGACCAGTTCCTTCAAAATTTC CTCAAAGGAGAAGTGCAGTTTGGCTCCTGGTTTGACCACATCAAGGGCTGGATCCGGATGCAGAACCAAG AGAACTTCCTGTTTATCACCTATGAGGAGCTGCAGCAGGACCTGCGAGGCTCCGTGCAACGCATCTGTGA GTTCCTGGGCCGGCCACTGGGTGAAGAGGCCCTGAGCTCTGTGGTGGCCCACTCAGCCTTTGCTGCCATG AAGGCCAATACCATGTCCAACTACTCGCTGCTGCCGGCCAGCCTGCTGGACCACCGCCAGGGGGAGTTCC TGCGCAAAGGGATCAGTGGCGACTGGAAGAACCACTTCACTGTGGCCCAGAGTGAGGCTTTTGACAGTGT TTACCGAGAGCAAATGCACGGGGTGCAGAGGTTCCCCTGGGACACGTCTGAAGAGGATAGCAGCCCTGAT GGCCAGCCTGACCCTGAGCCCAGCCCCAGCCCAGCTTCTGATGACCCCAACCCAGGATCCTCACAATAAA CTTATCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC009813
Insert Size	1017 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009813, AAH09813
RefSeq Size	1094 bp
RefSeq ORF	1017 bp
Locus ID	54200
Gene Summary	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids such as dehydroepiandrosterone. Isoform 1 is required for production of cholesterol sulfate essential for normal skin development whereas isoform 2 produces pregnenolone sulfate, an essential neurosteroid during development of the central nervous system. Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation.[UniProtKB/Swiss-Prot Function]

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