Human Angiogenin (ANG) ELISA KIT (96 T)

Specifications

Product Data
Description	Human Angiogenin Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	7.8-500 pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human Angiogenin.
Sensitivity	4 pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,1000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Human Angiogenin (ANG) is a single chain, 14 kDa, non-glycosylated polypeptide that is classified as a member of the RISBASE family of ribonucleases. RISBASE family molecules exhibit both ribonuclease activity and special biological actions. ANG is 123 amino acid (aa) residues in length and contains three intra-chain disulfide bonds. When optimally aligned with pancreatic ribonuclease, ANG shows 35% sequence identity. This is reflected in its modest RNA catalytic activity. It has been suggested that angiogenin binding may result in significant RNase activity. Relative to human ANG, mouse ANG is 75% identical at the aa level and human ANG is reported to be active in the murine system. Cells known to express ANG include vascular endothelial cells, smooth muscle cells, fibroblasts, colonic columnar epithelium, lymphocytes, and primary adenocarcinoma cells as well as select tumor cell lines. The receptor for angiogenin is unknown, however, it would appear that actin, either as a receptor or as a binding molecule, is essential for the expression of angiogenin’s effects.
Gene Symbol	ANG

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