Human AKR1C3 ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human AKR1C3 in cell culture supernates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human AKR1C3
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156 pg/ml-10.000pg/ml
Specificity	This kit is used for quantitative detection of Human AKR1C3
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human AKR1C3 antibody 1 Lyophilized recombinant human AKR1C3 standard 10ng/tubex2 Biotinylated anti- human AKR1C3 antibody 100ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 100ul( dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml Plate Sealers 4 x Wash Buffer (25x) 20 ml
Background	Aldo-keto reductase family 1 member C3 (AKR1C3), also known as 17β-hydroxysteroid dehydrogenase type 5 (17β-HSD5, HSD17B5) is a key steroidogenic enzyme that in humans is encoded by the AKR1C3 gene. It is mapped to 10p15.1. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene.
Gene Symbol	AKR1C3

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.