GAPDHS Mouse Monoclonal Antibody [Clone ID: Hs-8]
CAT#: SM3115P
GAPDHS mouse monoclonal antibody, clone Hs-8, Purified
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CNY 3,540.00
货期*
5周
规格
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Specifications
Product Data | |
Clone Name | Hs-8 |
Applications | FC, IF, WB |
Recommend Dilution | Immunocytochemistry: 10 µg/ml, membrane permeabilization (acetone) essential. Flow cytometry. Western blot. |
Reactivity | Human, Porcine |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
Specificity | The antibody reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, previously identified under the general name "intra-acrosomal protein". |
Formulation | Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 State: Purified State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Precipitation methods and size-exclusion chromatography; purity: > 95% (by SDS-PAGE) |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C. DO NOT FREEZE! |
Gene Name | glyceraldehyde-3-phosphate dehydrogenase, spermatogenic |
Database Link | |
Background | GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction. |
Synonyms | GAPDHS, GAPD2, GAPDS, HSD35, HSD-35 |
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