Macrophage Inflammatory Protein 3 alpha (CCL20) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human MIP-3 alpha (100 ng/ml), a concentration of 15.0 - 18.0 µg/ml of this antibody is required. ELISA: To detect MIP-3 alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antibody allows the detection of 0.2 - 0.4 ng/well of recombinant MIP-3 alpha. Western Blot: This antibody can be used at a concentration of 0.1 - 0.2 µg/ml The detection limit for recombinant MIP-3 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on frozen (use 1 µg/ml) and paraffin embedded tissues.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant human Macrophage Inflammatory Protein 3 alpha (hMIP-3 alpha).
Specificity	Human MIP-3 alpha (EXODUS-1, LARC).
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 20
Database Link	Entrez Gene 6364 Human UniProt ID P78556
Background	Macrophage Inflammatory Protein 3 alpha is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. It inhibits proliferation of myeloid progenitors in colony formation assays. It may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. It binds to CCR6.
Synonyms	CCL20, LARC, MIP3A, MIP-3 alpha, C-C motif chemokine 20, Small-inducible cytokine A20, SCYA20, Exodus-1, Dendritic Cell Marker, DC marker
Note	Centrifuge vial prior to opening!
Reference Data

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