MEK6 (MAP2K6) pSer207 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry on Paraffin Sections: 1/50-1/100. Immunofluorescence: 1/100-1/200.
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from Human MKK6 around the phosphorylation site of Serine 207 (V-D-Sp-V-A).
Specificity	This antibody detects endogenous levels of MKK6 only when phosphorylated at serine207.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	mitogen-activated protein kinase kinase 6
Database Link	Entrez Gene 5608 Human UniProt ID P52564
Background	Mitogen activated protein kinase kinase 6 (MEK6 or MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MEK6, closely related to MEK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38, thus activating it, in response to inflammatory cytokines and environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this protein is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
Synonyms	MAPKK 6, MAPK/ERK kinase 6, SAPKK3, MEK6, MKK6, MAP kinase kinase 6
Reference Data

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