CCN2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human CTGF (using 100 μl/well antibody solution) a concentration of Approx. 1 μg/ml of this antibody is required. This Biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2–0.4 ng/well of recombinant Human CTGF. Sandwich ELISA: To detect Human CTGF (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human CTGF (AP01150PU-N or AP01150PU-S) as a Capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human CTGF. Western blot: To detect Human CTGF this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) recombinant Human CTGF
Specificity	This antibody detects Connective Tissue Growth Factor.
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. Store the reconstituted antibody at 2-8°C for one month or at -20°C (in aliquots) for longer. Avoid repeated freezing and thawing.
Gene Name	connective tissue growth factor
Database Link	Entrez Gene 1490 Human UniProt ID P29279
Background	CTGF (connective tissue growth factor) is a 38kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.
Synonyms	CCN2, HCS24, IGFBP8, Connective tissue growth factor, IGF-binding protein 8,IBP8
Reference Data

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