Laminin beta 1 (LAMB1) Mouse Monoclonal Antibody [Clone ID: DG10]

Specifications

Product Data
Clone Name	DG10
Applications	ELISA, IHC, WB
Recommend Dilution	Western blotting:  Clone DG10 against Laminin ß1-chain is apparently one of the few laminin antibodies working perfectly in Immunoblotting, thus making it especially valuable for biochemical studies.  Immunohistochemical on Frozen Sections Other immunoassays for cellular and tumor biology. Unlike many other Monoclonal antibodies to Ln β1 chain this Mab clone DG10 reacts strongly both under reducing and non-reducing conditions. Clone DG10 has been successfully used to purify Ln-8 (ref.2) and Ln-10 (ref.6) by Affinity Chromatography.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Partially pepsin-digested laminins from Human placenta (ref.8). Hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
Specificity	The antibody recognizes the Mr 220000 β1-chain of laminin. Clone DG10 appears to react with the NH2-terminus of the Ln β1 chain (1). The antibody reacts with Human tissues.
Formulation	PBS State: Ig Fraction State: Liquid Ig fraction Stabilizer: 1.0% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	laminin subunit beta 1
Database Link	Entrez Gene 3912 Human UniProt ID P07942
Background	Laminin, a major structural glycoprotein of the basement membranes, is an attachment and survival factor for epithelial cells and can also promote the growth of epithelial cells. Laminin plays an important role in both the structural organization of basement membranes and in the anchorage of cells. The monoclonal antibody, DG10 and BC17 against laminin chain ß1 and γ1, respectively can be widely applied in immunohistochemical studies as all basement membranes contain laminin trimers containing these chains. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning, for instance, the integrity of the basement membranes in carcinomas. Clone DG10 against Laminin ß1-chain is apparently one of the few laminin antibodies working perfectly in immunoblotting, thus making it especially valuable for biochemical studies.
Synonyms	Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta
Reference Data

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