Glycogen Synthase Kinase-3 (GSK3) (phospho specific) Mouse Monoclonal Antibody [Clone ID: 7G-1F2]

Specifications

Product Data
Clone Name	7G-1F2
Applications	IHC
Recommend Dilution	Immunohistochemistry on paraffin sections: 1 µg/ml (1:200). Microwave pretreatment in citrate buffer is recommended for antigen retrieval. Suggested positive control: Human brain.
Reactivity	Human, Porcine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugate corresponding to Drosophila
Specificity	This antibody recognizes GSK-3 in most species. The antibody was generated against the phosphorylated catalytic domain (between sub-domain VII and VIII) of the Drosophila GSK-3 / shaggy enzyme, corntaining the phospho-Tyrosine 279 of h uman GSK-3α and phospho-Tyrosine 216 of human GSK-3β, respectively. This antibody does not react with GSK-3 or shaggy expressed in E. coli, but will react with the corresponding natural enzyme or the one expressed in Sf9 cells. The observed preference for the phosphorylated form is about 50:1. The antigen has been described in the testis, thymus, prostate and ovary, weak in the brain, lung and kidney.
Formulation	Phosphate buffered saline pH 7.2 (PBS) State: Purified State: Lyophilized affinity purified Ig fraction Stabilizer: 5 mg/ml bovine serum albumin (BSA) Preservative: 0.01% Kathon
Reconstitution Method	Reconstitute by adding 0.5 ml distilled water. This stock solution contains 0.2 mg/ml IgG.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	GSK-3 has been highly conserved during evolution, and homolog genes have been identified in virtually every eukaryotic genome investigated. In mam mals, GSK-3 is encoded by two genes known as gsk-3 α and gsk-3β encoding GSK-3α (483 aa in humans) and GSK-3β (433 aa) proteins with apparent molecular masses of 51 and 47kDa, respectively. Both isoforms are almost identical (98%) within their ATP binding p ocket but differ in their N- and C- terminal sequences.
Reference Data

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