Chk1 (CHEK1) Mouse Monoclonal Antibody [Clone ID: 2G1D5]

Specifications

Product Data
Clone Name	2G1D5
Applications	WB
Recommend Dilution	ELISA: 1/10,000. Western blotting: 1/1,000-1/4,000.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	This monoclonal antibody is generated from mice immunized with Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15.
Specificity	This antibody is specific to ChK1.
Formulation	PBS containing 0.09% (W/V) Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	checkpoint kinase 1
Database Link	Entrez Gene 1111 Human UniProt ID O14757
Background	Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The protein exhibits altered mobility in immunoblotting when isolated from cells treated with ionizing radiation (IR), indicating that CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. Evidence indicates that in response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. Proteolysis of activated CHK1 may promote checkpoint termination under normal conditions and may play a role in the cytotoxic effects of camptothecin and related anticancer drugs.
Synonyms	CHEK1, CHEK-1
Reference Data

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