MHV NSP9 (Strain A59) Mouse Monoclonal Antibody [Clone ID: 2C6.H1]

Specifications

Product Data
Clone Name	2C6.H1
Applications	IF, WB
Recommend Dilution	Western blot: 1:1,000 (Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 in the appropriate cell lysate or extract). Immunofluorescence: 1:1,000 (Vero-E6 cells were grown on glass slides followed by infection with MHVA59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min).
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	E. coli derived fulllength MHV-A59 nsp9 protein.
Specificity	This antibody is directed against the MHV-A59 nsp9 protein.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C or below for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Database Link	UniProt ID P0C6X9
Background	The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Synonyms	Nonstructural Protein 9, Mouse Hepatitis Virus Strain A59, Murine coronavirus
Reference Data

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