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OriGene proteins in recent publications

Fully functional proteins purified from transient TrueORF cDNA clone transfections.  Researchers purified GEN1 protein using anti-FLAG M2 affinity column and determined it resolved Holliday junction X0 efficiently in vitro, thus confirming GEN1 is human Holliday junction resolvase.

Identification of Holliday junction resolvases from humans and yeast, Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel, Stephen C. West, Nature 456, 357 - 361 (20 Nov 2008), doi: 10.1038/nature07470,


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BMP2 (NM_001200) Purified Human Protein

Specifications Citations (1) Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP750012 Recombinant protein of human Bone Morphogenetic Protein-2 (BMP2) produced in E. coli., 20 ug (Larger pack size?) ¥3791.00 现货
TA50011-100 4C5, Anti-DDK monoclonal antibody (100 μL) ¥1000 现货
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Also for BMP2 (NM_001200)

OriGene Data
Species:Human Expression Host:E. coli
Expression cDNA Clone or AA Sequence
A DNA sequence from TrueORF clone, RC214586, encoding the region (Gln283-Arg396) of human BMP2
Tag:Tag-free Predicted MW:13 kDa
Purity:>95% as determined by SDS-PAGE and Coomassie blue staining
Concentration:Resuspend the protein to the desired concentration in proper buffer.
Buffer:Lyophilized from a sterile solution containing 10mM sodium citrate, pH 3.5. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Endotoxin: <0.1 EU per 1 ?g of the protein by the LAL
BioActivity: Measured?by?its?ability?to?induce?alkaline?phosphatase?production in?ATDC5?cells. ED50 for this effect is typically 36 - 55 ng/ml.
Protein Families: Secreted Protein
ES Cell Differentiation/IPS
Transmembrane
Adult stem cells
Cancer stem cells
Embryonic stem cells
Induced pluripotent stem cells
Stem cell relevant signaling - TGFb/BMP signaling pathway
Druggable Genome
Protein Pathways: Cytokine-cytokine receptor interaction
Hedgehog signaling pathway
TGF-beta signaling pathway
Pathways in cancer
Basal cell carcinoma
Protein Data
  Protein Image
 

Reference Data
RefSeq: NP_001191 RefSeq Size: 1547 RefSeq ORF: 1191
LocusID: 650 Cytogenetic: 20p12.3  
Synonyms : BDA2; BMP2A
Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag? is a registered trademark of Sigma-Aldrich

 

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