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OriGene proteins in recent publications

Fully functional proteins purified from transient TrueORF cDNA clone transfections.  Researchers purified GEN1 protein using anti-FLAG M2 affinity column and determined it resolved Holliday junction X0 efficiently in vitro, thus confirming GEN1 is human Holliday junction resolvase.

Identification of Holliday junction resolvases from humans and yeast, Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel, Stephen C. West, Nature 456, 357 - 361 (20 Nov 2008), doi: 10.1038/nature07470,


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FGF10 (NM_004465) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP750011 Recombinant protein of human Fibroblast Growth Factor-10 (FGF10) produced in E. coli., 100 ug (Larger pack size?) ¥3791.00 现货
TA50011-100 4C5, Anti-DDK monoclonal antibody (100 μL) ¥1000 现货
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Also for FGF10 (NM_004465)

OriGene Data
Species:Human Expression Host:E. coli
Expression cDNA Clone or AA Sequence
A DNA sequence from TrueORF clone, RC210120, encoding the region (Leu40-Ser208) of human FGF10
Tag:Tag-free Predicted MW:19.3 kDa
Purity:>95% as determined by SDS-PAGE and Coomassie blue staining
Concentration:Resuspend the protein to the desired concentration in proper buffer.
Buffer:Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.4. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Endotoxin: <0.1 EU per 1 ?g of the protein by the LAL
BioActivity: Determined by the dose-dependant stimulation of 4MBr-5 cells. ED50 for this effect is typically 14 - 21 ng/ml.
Protein Families: Secreted Protein
ES Cell Differentiation/IPS
Transmembrane
Adult stem cells
Embryonic stem cells
Transcription Factors
Druggable Genome
Protein Pathways: MAPK signaling pathway
Regulation of actin cytoskeleton
Pathways in cancer
Melanoma
Protein Data
  Protein Image
 

Reference Data
RefSeq: NP_004456 RefSeq Size: 627 RefSeq ORF: 627
LocusID: 2255 Cytogenetic: 5p12  
Synonyms : keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria; fibroblast growth factor 10
Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq, Jul 2008]
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag? is a registered trademark of Sigma-Aldrich

 

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