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OriGene proteins in recent publications

Fully functional proteins purified from transient TrueORF cDNA clone transfections.  Researchers purified GEN1 protein using anti-FLAG M2 affinity column and determined it resolved Holliday junction X0 efficiently in vitro, thus confirming GEN1 is human Holliday junction resolvase.

Identification of Holliday junction resolvases from humans and yeast, Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel, Stephen C. West, Nature 456, 357 - 361 (20 Nov 2008), doi: 10.1038/nature07470,


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FGF7 (NM_002009) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP750010 Recombinant protein of human Fibroblast Growth Factor-7 (KGF) produced in E. coli., 20 ug (Larger pack size?) ¥3791.00 现货
TA50011-100 4C5, Anti-DDK monoclonal antibody (100 μL) ¥1000 现货
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Also for FGF7 (NM_002009)

OriGene Data
Species:Human Expression Host:E. coli
Expression cDNA Clone or AA Sequence
A DNA sequence from TrueORF clone, RC224418, encoding the region (Cys32-Thr194) of human FGF7
Tag:Tag-free Predicted MW:19 kDa
Purity:>95% as determined by SDS-PAGE and Coomassie blue staining
Concentration:Resuspend the protein to the desired concentration in proper buffer.
Buffer:Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.4. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Endotoxin: <0.1 EU per 1 ?g of the protein by the LAL
BioActivity: Determined by the dose-dependant stimulation of 4MBr-5 cells. ED50 for this effect is typically 0.92-1.38 ng/ml.
Protein Families: Secreted Protein
ES Cell Differentiation/IPS
Adult stem cells
Embryonic stem cells
Druggable Genome
Protein Pathways: MAPK signaling pathway
Regulation of actin cytoskeleton
Pathways in cancer
Melanoma
Protein Data
  Protein Image
 

Reference Data
RefSeq: NP_002000 RefSeq Size: 3853 RefSeq ORF: 585
LocusID: 2252 Cytogenetic: 15q21.2  
Synonyms : HBGF-7; KGF
Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag? is a registered trademark of Sigma-Aldrich

 

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